LOCUS       sequence-270810-       10416 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral inducible expression (TetOn) of human HEC1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10416)
  TITLE     de Carcer unpublished plasmids
REFERENCE   2  (bases 1 to 10416)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti_TetOn_Hu_HEC1(WT)
FEATURES             Location/Qualifiers
     source          1..10416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    36..54
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    71..89
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    107..125
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    143..161
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    178..196
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    214..232
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     279..303
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    384..408
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2371..2395)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2426..3014
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2479..2499)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2897..2908)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        3070..3569
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3105..3128)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3502..3521
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3587..4612
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             3587..4612
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             4986..5219
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5291..5412
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5328..5347)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5382..5401
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5452..5587
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5607..5626)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5608..5626)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5636..5653)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5636..5652)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5645..5667)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5794..6249
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5881..5900)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6091..6112
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6275..6379
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6380..6448,6449..7240)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6598..6617)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7411..7999
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7900..7919
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8153..8170
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8287..8308
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8323..8328,8329..8346,8347..8353)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8361..8377
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8366..8388
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8385..8401
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8385..8401
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8420..8440
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8422..8440
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8468..8694
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8695..8875
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8922..9047
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9540..9773
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9958..10002
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10269..10386
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(10408..10416,1..306)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    join(10416,1..18)
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgatg
       61 tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat
      121 agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttatcc
      181 ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt
      241 cgaggtaggc gtgtacggtg ggaggcctat ataagcagag ctcgtttagt gaaccgtcag
      301 atcgcctgga gaattcgagc tcggtacccg gggatccacc ggttcgtcga ctagtccagt
      361 gtggtggaat tctgcagata tcaacaagtt tgtacaaaaa agcaggctcc gcggccgccc
      421 ccttcaccat gaagcgcagt tcagtttcca gcggtggtgc tggccgcctc tccatgcagg
      481 agttaagatc ccaggatgta aataaacaag gcctctatac ccctcaaacc aaagagaaac
      541 caacctttgg aaagttgagt ataaacaaac cgacatctga aagaaaagtc tcgctatttg
      601 gcaaaagaac tagtggacat ggatcccgga atagtcaact tggtatattt tccagttctg
      661 agaaaatcaa ggacccgaga ccacttaatg acaaagcatt cattcagcag tgtattcgac
      721 aactctgtga gtttcttaca gaaaatggtt atgcacataa tgtgtccatg aaatctctac
      781 aagctccctc tgttaaagac ttcctgaaga tcttcacatt tctttatggc ttcctgtgcc
      841 cctcatacga acttcctgac acaaagtttg aagaagaggt tccaagaatc tttaaagacc
      901 ttgggtatcc ttttgcacta tccaaaagct ccatgtacac agtgggggct cctcatacat
      961 ggcctcacat tgtggcagcc ttagtttggc taatagactg catcaagata catactgcca
     1021 tgaaagaaag ctcaccttta tttgatgatg ggcagccttg gggagaagaa actgaagatg
     1081 gaattatgca taataagttg tttttggact acaccataaa atgctatgag agttttatga
     1141 gtggtgccga cagctttgat gagatgaatg cagagctgca gtcaaaactg aaggatttat
     1201 ttaatgtgga tgcttttaag ctggaatcat tagaagcaaa aaacagagca ttgaatgaac
     1261 agattgcaag attggaacaa gaaagagaaa aagaaccgaa tcgtctagag tcgttgagaa
     1321 aactgaaggc ttccttacaa ggagatgttc aaaagtatca ggcatacatg agcaatttgg
     1381 agtctcattc agccattctt gaccagaaat taaatggtct caatgaggaa attgctagag
     1441 tagaactaga atgtgaaaca ataaaacagg agaacactcg actacagaat atcattgaca
     1501 accagaagta ctcagttgca gacattgagc gaataaatca tgaaagaaat gaattgcagc
     1561 agactattaa taaattaacc aaggacctgg aagctgaaca acagaagttg tggaatgagg
     1621 agttaaaata tgccagaggc aaagaagcga ttgaaacaca attagcagag tatcacaaat
     1681 tggctagaaa attaaaactt attcctaaag gtgctgagaa ttccaaaggt tatgactttg
     1741 aaattaagtt taatcccgag gctggtgcca actgccttgt caaatacagg gctcaagttt
     1801 atgtacctct taaggaactc ctgaatgaaa ctgaagaaga aattaataaa gccctaaata
     1861 aaaaaatggg tttggaggat actttagaac aattgaatgc aatgataaca gaaagcaaga
     1921 gaagtgtgag aactctgaaa gaagaagttc aaaagctgga tgatctttac caacaaaaaa
     1981 ttaaggaagc agaggaagag gatgaaaaat gtgccagtga gcttgagtcc ttggagaaac
     2041 acaagcacct gctagaaagt actgttaacc aggggctcag tgaagctatg aatgaattag
     2101 atgctgttca gcgggaatac caactagttg tgcaaaccac gactgaagaa agacgaaaag
     2161 tgggaaataa cttgcaacgt ctgttagaga tggttgctac acatgttggg tctgtagaga
     2221 aacatcttga ggagcagatt gctaaagttg atagagaata tgaagaatgc atgtcagaag
     2281 atctctcgga aaatattaaa gagattagag ataagtatga gaagaaagct actctaatta
     2341 agtcttctga agaaaagggt gggcgcgccg acccagcttt cttgtacaaa gtggttgata
     2401 tccagcacag tggcggccgc tcgacaatca acctctggat tacaaaattt gtgaaagatt
     2461 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     2521 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     2581 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     2641 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     2701 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     2761 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     2821 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     2881 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     2941 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     3001 ggccgcctcc ccgcctggaa ttctgcagat atccggacta gtgatctctc gaggttaacg
     3061 aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag
     3121 ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca
     3181 ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc
     3241 ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg
     3301 aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg
     3361 taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc
     3421 tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc
     3481 gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt
     3541 tctcctcttc ctcatctccg ggcctttcga cctgcatccc gccaccatga aaaagcctga
     3601 actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct
     3661 gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg
     3721 atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg
     3781 gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga
     3841 gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga
     3901 aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc
     3961 cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac
     4021 tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt
     4081 gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc
     4141 cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct
     4201 gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc
     4261 ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca
     4321 gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta
     4381 tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga
     4441 tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg
     4501 gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact
     4561 cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat agagtagatg
     4621 ccgaccgaac aagagctgat ttcgagaacg cctcagccag caactcgcgc gagcctagca
     4681 aggcaaatgc gagagaacgg ccttacgctt ggtggcacag ttctcgtcca cagttcgcta
     4741 agctcgctcg gctgggtcgc gggagggccg gtcgcagtga ttcaggccct tctggattgt
     4801 gttggtcccc agggcacgat tgtcatgccc acgcactcgg gtgatctgac tgatcccgca
     4861 gattggagat cgccgcccgt gcctgccgat tgggtgcaga tccgtcgagg gcccggtacc
     4921 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     4981 gggactggaa gggctagctc actcccaacg aagacaagat ctgctttttg cttgtactgg
     5041 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     5101 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     5161 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     5221 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     5281 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     5341 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     5401 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
     5461 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     5521 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     5581 ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg
     5641 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     5701 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     5761 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     5821 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     5881 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     5941 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     6001 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     6061 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     6121 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     6181 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     6241 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     6301 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     6361 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     6421 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     6481 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     6541 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     6601 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     6661 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     6721 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     6781 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     6841 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     6901 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     6961 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     7021 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     7081 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     7141 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     7201 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     7261 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     7321 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     7381 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     7441 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     7501 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     7561 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     7621 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     7681 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     7741 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     7801 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     7861 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     7921 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     7981 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     8041 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     8101 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     8161 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     8221 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     8281 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     8341 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     8401 ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca
     8461 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
     8521 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
     8581 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
     8641 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
     8701 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     8761 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     8821 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     8881 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     8941 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     9001 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     9061 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     9121 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     9181 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     9241 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     9301 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     9361 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     9421 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     9481 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     9541 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     9601 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     9661 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     9721 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     9781 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     9841 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     9901 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     9961 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
    10021 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
    10081 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
    10141 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
    10201 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
    10261 cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
    10321 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
    10381 aattttatcg atcacgagac tagcctcgag tttact
//
