LOCUS       sequence-270811-        8916 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral inducible expression (TetOn) of human BIRC5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8916)
  TITLE     de Carcer unpublished plasmids
REFERENCE   2  (bases 1 to 8916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLENTI_TetOn_Hu_BIRC5(WT)
FEATURES             Location/Qualifiers
     source          1..8916
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    8..24
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13..35
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     32..48
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     32..48
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     67..87
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        69..87
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        115..341
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             342..522
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    569..694
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1187..1420
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1605..1649
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1916..2033
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2055..2369
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2063..2081
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2099..2117
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2134..2152
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2170..2188
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2206..2224
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2241..2259
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2277..2295
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2342..2366
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2447..2471
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2934..2958)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2989..3577
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3042..3062)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3460..3471)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        3633..4132
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3668..3691)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4065..4084
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4150..5175
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             4150..5175
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             5549..5782
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5854..5975
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5891..5910)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5945..5964
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6015..6150
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6170..6189)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6171..6189)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6199..6216)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6199..6215)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6208..6230)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6357..6812
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6444..6463)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6654..6675
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6838..6942
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6943..7011,7012..7803)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7161..7180)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7974..8562
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8463..8482
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8716..8733
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8850..8871
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8886..8891,8892..8909,8910..8916)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
       61 aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt
      121 cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac
      181 aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct
      241 tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt
      301 gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc
      361 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
      421 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
      481 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
      541 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
      601 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
      661 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
      721 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
      781 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
      841 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
      901 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
      961 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     1021 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     1081 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     1141 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     1201 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     1261 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     1321 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     1381 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     1441 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     1501 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     1561 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     1621 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     1681 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     1741 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     1801 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     1861 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgg ttaactttta
     1921 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     1981 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttatcgatc
     2041 acgagactag cctcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc
     2101 cctatcagtg atagagaacg atgtcgagtt tactccctat cagtgataga gaacgtatgt
     2161 cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata
     2221 gagaacgtat gtcgagttta tccctatcag tgatagagaa cgtatgtcga gtttactccc
     2281 tatcagtgat agagaacgta tgtcgaggta ggcgtgtacg gtgggaggcc tatataagca
     2341 gagctcgttt agtgaaccgt cagatcgcct ggagaattcg agctcggtac ccggggatcc
     2401 accggttcgt cgactagtcc agtgtggtgg aattctgcag atatcaacaa gtttgtacaa
     2461 aaaagcaggc tccgcggccg cccccttcac catgggtgcc ccgacgttgc cccctgcctg
     2521 gcagcccttt ctcaaggacc accgcatctc tacattcaag aactggccct tcttggaggg
     2581 ctgcgcctgc accccggagc ggatggccga ggctggcttc atccactgcc ccactgagaa
     2641 cgagccagac ttggcccagt gtttcttctg cttcaaggag ctggaaggct gggagccaga
     2701 tgacgacccc atagaggaac ataaaaagca ttcgtccggt tgcgctttcc tttctgtcaa
     2761 gaagcagttt gaagaattaa cccttggtga atttttgaaa ctggacagag aaagagccaa
     2821 gaacaaaatt gcaaaggaaa ccaacaataa gaagaaagaa tttgaggaaa ctgcgaagaa
     2881 agtgcgccgt gccatcgagc agctggctgc catggataag ggtgggcgcg ccgacccagc
     2941 tttcttgtac aaagtggttg atatccagca cagtggcggc cgctcgacaa tcaacctctg
     3001 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     3061 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     3121 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     3181 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     3241 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     3301 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     3361 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     3421 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     3481 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     3541 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattctgca gatatccgga
     3601 ctagtgatct ctcgaggtta acgaattcta ccgggtaggg gaggcgcttt tcccaaggca
     3661 gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg
     3721 gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc
     3781 cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc
     3841 gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag
     3901 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc
     3961 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg
     4021 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca
     4081 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcat
     4141 cccgccacca tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa
     4201 aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc
     4261 agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc
     4321 tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg
     4381 cttgacattg gggaattcag cgagagcctg acctattgca tctcccgccg tgcacagggt
     4441 gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag
     4501 gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga
     4561 ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc
     4621 catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct
     4681 ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg
     4741 gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg
     4801 agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg
     4861 tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca
     4921 ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc
     4981 ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc
     5041 cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg
     5101 accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg
     5161 agggcaaagg aatagagtag atgccgaccg aacaagagct gatttcgaga acgcctcagc
     5221 cagcaactcg cgcgagccta gcaaggcaaa tgcgagagaa cggccttacg cttggtggca
     5281 cagttctcgt ccacagttcg ctaagctcgc tcggctgggt cgcgggaggg ccggtcgcag
     5341 tgattcaggc ccttctggat tgtgttggtc cccagggcac gattgtcatg cccacgcact
     5401 cgggtgatct gactgatccc gcagattgga gatcgccgcc cgtgcctgcc gattgggtgc
     5461 agatccgtcg agggcccggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     5521 agccactttt taaaagaaaa ggggggactg gaagggctag ctcactccca acgaagacaa
     5581 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     5641 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     5701 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     5761 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     5821 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     5881 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     5941 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     6001 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     6061 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     6121 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
     6181 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     6241 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     6301 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     6361 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     6421 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     6481 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     6541 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     6601 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     6661 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     6721 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     6781 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     6841 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     6901 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     6961 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     7021 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     7081 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     7141 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     7201 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     7261 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     7321 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     7381 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     7441 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     7501 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     7561 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     7621 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     7681 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     7741 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     7801 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     7861 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     7921 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     7981 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     8041 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     8101 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     8161 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     8221 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     8281 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     8341 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     8401 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     8461 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     8521 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     8581 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     8641 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     8701 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     8761 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     8821 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     8881 caggctttac actttatgct tccggctcgt atgttg
//
