LOCUS       sequence-270813-        3774 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mouse CCNB2 cDNA in Gateway pENTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3774)
  TITLE     de Carcer unpublished plasmids
REFERENCE   2  (bases 1 to 3774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pENTR_Hu_CCNB2(WT)
FEATURES             Location/Qualifiers
     source          1..3774
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1331..1430)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1447..1466)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1448..1466)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1471..1487)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1471..1487)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1589..1608)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1600..2409
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1667..1686)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2556..3144
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3045..3064
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3298..3315
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3474..3501)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3593..3679)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3728..3750
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3742..3759
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3743..3759
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atggcgctgc tccgacgccc gacggtgtcc agtgatttga agaatattga cacagaagtt
      181 attcctaaag ccaagagcca tgtgactatc cggcgggcag ttttagaaga aattggaaat
      241 aaagttagaa acagaaccac tcaggtggcg aagaaacctc agaacaccaa agtaccagct
      301 ctgcccacca aagtgacaaa tgtcaacaag cagccgaaac ccacagcctc tgtgaaacca
      361 gtgcagatgg aggcgctagc tcccaaggat cgtcctcccg cccctgagga tgtctccatg
      421 aaggaagaga gcctctgcca agctttctct gatgctctgc tctgcaagat cgaggacata
      481 gataacgagg acagggagaa ccctcagctc tgcagtgact acgtgaagga catctaccag
      541 tacctcaggc agcttgaggt tttacagtcc attaatccac acttcttaga tggaagagat
      601 ataaatggac gtatgcgtgc catcctggtg gactggctgg tccaagtcca ttccaagttt
      661 cggcttctgc aggaaactct gtacatgtgc attgccatca tggaccggtt cctgcaggct
      721 cagctggtct gccggaagaa actgcagctg gtcgggatca cagctctgct cctggcttcc
      781 aaatatgagg agatgttttc tccaaatatc gaagactttg tttatatcac agataatgct
      841 tacaccagtt cccaaatccg agaaatggag actctgattt tgaaagagtt gaaatttgag
      901 ttgggccgac ctttgccact tcacttctta aggcgagcct caaaagccgg agaggtggat
      961 gttgaacagc acactttagc caagtacctg atggagctga cgctcgtcga ctatgacatg
     1021 gtgcactacc atccttctca ggtggcagcc gctgcctctt gcctgtctca gaaggtgctg
     1081 ggccaaggaa aatggaattt gaagcagcag tattacacag gctacatgga gagtgaagtc
     1141 ctggaagtca tgcagcacat ggccaagaac gttgtgaaag tcaatgacaa ccgtaccaag
     1201 ttcatcgctg tcaagaacaa gtatgccagc agcagactcc tgaagatcag cacgatccct
     1261 cagctgaact ccaaaatcat caaagacctg gcctcccctc tgctgggcag ccccaagggt
     1321 gggcgcgccg acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca
     1381 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct
     1441 gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc
     1501 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata
     1561 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa
     1621 acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
     1681 cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg
     1741 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     1801 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
     1861 actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta
     1921 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     1981 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc
     2041 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
     2101 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca
     2161 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     2221 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     2281 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     2341 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     2401 tttttctaat cagaattggt taattggttg taacactggc agagcattac gctgacttga
     2461 cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc gttccactga
     2521 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     2581 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     2641 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     2701 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     2761 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     2821 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     2881 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     2941 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     3001 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     3061 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     3121 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     3181 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     3241 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     3301 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     3361 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     3421 cgcaacgcaa ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc
     3481 catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg
     3541 cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc
     3601 tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact
     3661 gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg
     3721 atgttttccc agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gccc
//
