LOCUS       sequence-270818-        6162 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LAMP1 (Lysosome Membrane) labeling with near-infrared fluorescent 
            protein emiRFP670 (enhanced miRFP670).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6162)
  AUTHORS   Matlashov ME, Shcherbakova DM, Alvelid J, Baloban M, Pennacchietti 
            F, Shemetov AA, Testa I, Verkhusha VV
  TITLE     A set of monomeric near-infrared fluorescent proteins for multicolor
            imaging across scales.
  JOURNAL   Nat Commun. 2020 Jan 13;11(1):239. doi: 10.1038/s41467-019-13897-6.
  PUBMED    31932632
REFERENCE   2  (bases 1 to 6162)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-01-13"; volume: "11(1):239. doi"; pages: " 
            10.1038/s41467-019-13897-6"
COMMENT     Sequence Label: pLAMP1-emiRFP670
FEATURES             Location/Qualifiers
     source          1..6162
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        133..436
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        437..640
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     591..611
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      692..701
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      696..705
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(698..760,761..1918)
                     /codon_start=1
                     /product="rat lysosome-associated membrane glycoprotein 1"
                     /label=LAMP1
                     /translation="MAAPGARRPLLLLLLAGLAHSAPALFEVKDNNGTACIMASFSASF
                     LTTYEAGHVSKVSNMTLPASAEVLKNSSSCGEKNASEPTLAITFGEGYLLKLTFTKNTT
                     RYSVQHMYFTYNLSDTQFFPNASSKGPDTVDSTTDIKADINKTYRCVSDIRVYMKNVTI
                     VLWDATIQAYLPSSNFSKEETRCPQDQPSPTTGPPSPSPPLVPTNPSVSKYNVTGDNGT
                     CLLASMALQLNITYMKKDNTTVTRAFNINPSDKYSGTCGAQLVTLKVGNKSRVLELQFG
                     MNATSSLFFLQGVQLNMTLPDAIEPTFSTSNYSLKALQASVGNSYKCNSEEHIFVSKAL
                     ALNVFSVQVQAFRVESDRFGSVEECVQDGNNMLIPIAVGGALAGLVLIVLIAYLIGRKR
                     SHAGYQTI"
     regulatory      1973..1982
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3022..3143
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3059..3078)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3113..3132
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3150..3605)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3287..3308)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3499..3518
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3632..3736
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3738..4095
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3738..3929
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3761..3781)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3946..4081
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4130..4924
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4130..4924
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4184..4203)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4794..4813
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5112..5131)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5156..5203
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5532..6120
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6021..6040
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
       61 accgccatgc attagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      121 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      181 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      241 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      301 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      361 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      421 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      481 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      541 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      601 cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat
      661 ccgctagcgc taccggactc agatctcgag cgccaccatg gcggccccgg gcgcccggcg
      721 tccgctgctc ctgttgctgc tggcaggcct tgcacacagc gccccagcac tgttcgaggt
      781 gaaagacaac aacggcacag cgtgtataat ggccagcttc tctgcctcct ttctgaccac
      841 ctatgaggct ggacatgttt ctaaggtctc gaatatgacc ctgccagcct ctgcagaagt
      901 cctgaagaat agcagctctt gtggtgaaaa gaatgcttct gagcccaccc tcgcaatcac
      961 ctttggagaa ggatatttac tgaaactcac cttcacaaaa aacacaacac gttacagtgt
     1021 ccagcacatg tatttcacat ataacctgtc agacacacaa ttctttccca atgccagctc
     1081 caaagggccc gacactgtgg attccacaac tgacatcaag gcagacatca acaaaacata
     1141 ccgatgtgtc agcgacatca gggtctacat gaagaatgtg accattgtgc tctgggacgc
     1201 tactatccag gcctacctgc cgagtagcaa cttcagcaag gaagagacac gctgcccaca
     1261 ggatcaacct tccccaacta ctgggccacc cagcccctca ccaccacttg tgcccacaaa
     1321 ccccagtgtg tccaagtaca atgtgactgg tgacaatgga acctgcctgc tggcctctat
     1381 ggcactgcaa ctcaacatca cctacatgaa gaaggacaac acgactgtga ccagagcatt
     1441 caacatcaac ccaagtgaca aatatagtgg gacttgcggt gcccagttgg tgaccctgaa
     1501 ggtggggaac aagagcagag tcctggagct gcagtttggg atgaatgcca cttctagcct
     1561 gtttttcctg caaggagttc agttgaacat gactcttcct gatgccatag agcccacgtt
     1621 cagcacctcc aactattccc tgaaagctct tcaggccagt gtcggcaact catacaagtg
     1681 caacagtgag gagcacatct ttgtcagcaa ggcgctcgcc ctcaatgtct tcagcgtgca
     1741 agtccaggct ttcagggtag aaagtgacag gtttgggtct gtggaagagt gtgtacagga
     1801 cggtaacaac atgctgatcc ccattgctgt gggcggggcc ctggcagggc tggtcctcat
     1861 cgtcctcatc gcctacctca tcggcaggaa gaggagtcac gcgggctatc agaccatctc
     1921 ggaattcggc tccaccggct ccaccggctc caccggcgcg gatccaccgg tcgccaccat
     1981 ggcggaaggc tccgtcgcca ggcagcctga cctcttgacc tgcgaacatg aagagatcca
     2041 cctcgccggc tcgatccagc cgcatggcgc gcttctggtc gtcagcgaac atgatcatcg
     2101 cgtcatccag gccagcgcca acgccgcgga atttctgaat ctcggaagcg tactcggcgt
     2161 tccgctcgcc gagatcgacg gcgatctgtt gatcaagatc ctgccgcatc tcgatcccac
     2221 cgccgaaggc atgccggtcg cggtgcgctg ccggatcggc aatccctcta cggagtactg
     2281 cggtctgatg catcggcctc cggaaggcgg gctgatcatc gaactcgaac gtgccggccc
     2341 gtcgatcgat ctgtcaggca cgctggcgcc ggcgctggag cggatccgca cggcgggttc
     2401 actgcgcgcg ctgtgcgatg acaccgtgct gctgtttcag cagtgcaccg gctacgaccg
     2461 ggtgatggtg tatcgtttcg atgagcaagg ccacggcctg gtattctccg agtgccatgt
     2521 gcctgggctc gaatcctatt tcggcaaccg ctatccgtcg tcgactgtcc cgcagatggc
     2581 gcggcagctg tacgtgcggc agcgcgtccg cgtgctggtc gacgtcacct atcagccggt
     2641 gccgctggag ccgcggctgt cgccgctgac cgggcgcgat ctcgacatgt cgggctgctt
     2701 cctgcgctcg atgtcgccgt gccatctgca gttcctgaag gacatgggcg tgcgcgccac
     2761 cctggcggtg tcgctggtgg tcggcggcaa gctgtggggc ctggttgtct gtcaccatta
     2821 tctgccgcgc ttcatccgtt tcgagctgcg ggcgatctgc aaacggctcg ccgaaaggat
     2881 cgcgacgcgg atcaccgcgc ttgagagcta atctagatca taatcagcca taccacattt
     2941 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     3001 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     3061 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     3121 tccaaactca tcaatgtatc ttaaggcgta aattgtaagc gttaatattt tgttaaaatt
     3181 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     3241 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     3301 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     3361 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     3421 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     3481 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     3541 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     3601 cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     3661 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     3721 ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg
     3781 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     3841 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     3901 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     3961 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     4021 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     4081 ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     4141 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     4201 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     4261 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc
     4321 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     4381 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     4441 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     4501 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     4561 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     4621 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct
     4681 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     4741 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     4801 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     4861 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     4921 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
     4981 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     5041 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg
     5101 gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca
     5161 ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg
     5221 tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc
     5281 gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc
     5341 aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat
     5401 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     5461 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     5521 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     5581 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5641 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     5701 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     5761 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     5821 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     5881 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     5941 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6001 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6061 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6121 aacgccagca acgcggcctt tttacggttc ctggcctttt gc
//
