LOCUS       sequence-270824-        3185 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AtzA expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Silverman AD, Akova U, Alam KK, Jewett MC, Lucks JB
  TITLE     Design and Optimization of a Cell-Free Atrazine Biosensor.
  JOURNAL   ACS Synth Biol. 2020 Mar 20;9(3):671-677. doi: 
            10.1021/acssynbio.9b00388. Epub 2020 Mar 3.
  PUBMED    32078765
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00388"; journalName: "ACS Synth Biol"; date: 
            "2020-03-20- 20"; volume: "9"; issue: "3"; pages: "671-677"
COMMENT     Sequence Label: pJBL7034
FEATURES             Location/Qualifiers
     source          1..3185
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1554..1572)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1568..1615
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(1633..2448)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2356..2375
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2440..2459
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      2570..3158
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3059..3078
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgttacta
       61 gagtcaagag gaaaatgcaa acgctttcca ttcagcatgg taccttggtc acaatggacc
      121 agtatcgccg tgtcttaggc gattcgtggg tgcacgtgca agatggccgt atcgttgctt
      181 tgggggtcca tgcagaatcg gttcctccac ccgccgatcg cgtgatcgac gcgcgcggga
      241 aagtcgtgct gccaggattt attaatgcgc atacccatgt caaccagatt cttcttcgcg
      301 gcggaccgag tcacgggcgc caattttacg actggttatt taatgtagtc tacccagggc
      361 agaaggccat gcgtcccgag gacgtagctg tggcagtgcg cttgtactgt gcagaagctg
      421 ttcgttctgg catcactact atcaatgaaa atgctgatag cgctatttat ccaggcaata
      481 tcgaggctgc aatggctgtg tatggagaag ttggggttcg tgtcgtctat gcgcgtatgt
      541 tttttgaccg catggatggt cgcattcaag gttacgttga tgcgttgaag gcgcgtagtc
      601 cacaagtgga gctgtgttca attatggaag aaacagctgt tgcaaaggac cgcatcacgg
      661 cactttccga tcaatatcat gggactgcgg ggggccgtat ttcggtctgg cctgccccgg
      721 ctaccactac agccgttacc gtggagggta tgcgctgggc gcaggcattc gctcgtgacc
      781 gcgccgtcat gtggaccctg cacatggcgg agtcagacca cgatgagcgc attcacggta
      841 tgtcgcctgc cgaatacatg gagtgctacg gccttcttga cgagcgctta caggtggccc
      901 attgcgtgta ctttgatcgc aaggacgttc gcttactgca tcgccataac gttaaagtgg
      961 cctctcaggt cgtgagcaat gcgtatctgg gctccggcgt tgcgccggtt ccagagatgg
     1021 tggaacgtgg gatggcagtc ggaattggta ccgacaacgg caacagtaat gattcagtta
     1081 atatgattgg agatatgaaa ttcatggccc acatccatcg tgctgttcac cgtgatgccg
     1141 atgtgcttac acccgaaaag atcttagaga tggctacgat cgacggcgca cgcagtcttg
     1201 gtatggacca tgaaatcgga tcaattgaga ctggcaaacg tgctgactta atccttcttg
     1261 acttacgcca tccgcagacg actccccacc accacctggc agcgactatc gtcttccagg
     1321 catatggaaa tgaggtagac acggtactta tcgatggcaa tgtggtgatg gaaaaccgcc
     1381 gccttagttt ccttcccccg gagcgtgaat tagctttcct tgaagaagca caaagtcgcg
     1441 ccacagctat cctgcaacgc gcaaacatgg ttgccaaccc tgcatggcgt agcctttaat
     1501 aagtcgaccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
     1561 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
     1621 agccaattct gattagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc
     1681 aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc
     1741 gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac
     1801 atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc
     1861 atgagtgacg actgaatccg gtgagaatgg caaaagctta tgcatttctt tccagacttg
     1921 ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt
     1981 cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca
     2041 aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa tattttcacc
     2101 tgaatcagga tattcttcta atacctggaa tgctgttttc ccggggatcg cagtggtgag
     2161 taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag gcataaattc
     2221 cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc
     2281 atgtttcaga aacaactctg gcgcatcggg cttcccatac aatcgataga ttgtcgcacc
     2341 tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat ccatgttgga
     2401 atttaatcgc ggcttcgagc aagacgtttc ccgttgaata tggctcataa caccccttgt
     2461 attactgttt atgtaagcag acagttttat tgttcatgat gatatatttt tatcttgtgc
     2521 aatgtaacat cagagatttt gagacacaac gtgagatcaa aggatcttct tgagatcctt
     2581 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     2641 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     2701 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     2761 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     2821 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     2881 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     2941 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     3001 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3061 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     3121 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcgatcccgc
     3181 gaaat
//
