LOCUS       sequence-270831-        6320 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expression of the genetically encoded dopamine sensor 
            dLight1.3b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6320)
  AUTHORS   Patriarchi T, Cho JR, Merten K, Howe MW, Marley A, Xiong WH, Folk 
            RW, Broussard GJ, Liang R, Jang MJ, Zhong H, Dombeck D, von Zastrow 
            M, Nimmerjahn A, Gradinaru V, Williams JT, Tian L
  TITLE     Ultrafast neuronal imaging of dopamine dynamics with designed 
            genetically encoded sensors.
  JOURNAL   Science. 2018 Jun 29;360(6396). pii: science.aat4422. doi: 
            10.1126/science.aat4422. Epub 2018 May 31.
  PUBMED    29853555
REFERENCE   2  (bases 1 to 6320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2018-06-29"; volume: "360(6396). pii: science.aat4422. doi"; 
            pages: " 10.1126/science.aat4422. Epub 2018 May 31"
COMMENT     Sequence Label: pAAV-syn-dLight1.3b
FEATURES             Location/Qualifiers
     source          1..6320
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..448
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     primer_bind     1454..1475
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1581..1602)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1842..1861)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    2547..3135
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2600..2620)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3018..3029)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3138..3154)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3139..3155)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    3184..3305
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3195..3214)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3249..3268
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   3467..3596
                     /label=AAV2 ITR
     rep_origin      complement(3833..4421)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3913..3932)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4592..5383,5384..5452))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5215..5234
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5453..5557)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5583..6038)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5720..5741)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5932..5951
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   6110..6239
                     /label=AAV2 ITR
ORIGIN
        1 agtgcaagtg ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga
       61 ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga
      121 gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg
      181 gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc
      241 gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg
      301 cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg
      361 cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg
      421 aggagtcgtg tcgtgcctga gagcgcagtc gaattcaagc tgctagcaag gatccaccgg
      481 tgcgcgccat gaagacgatc atcgccctga gctacatctt ctgcctggtg ttcgccgact
      541 acaaggacga tgatgacgcc atgaggactc tgaacacctc tgccatggac gggactgggc
      601 tggtggtgga gagggacttc tctgttcgta tcctcactgc ctgtttcctg tcgctgctca
      661 tcctgtccac gctcctgggg aacacgctgg tctgtgctgc cgttatcagg ttccgacacc
      721 tgcggtccaa ggtgaccaac ttctttgtca tctccttggc tgtgtcagat ctcttggtgg
      781 ccgtcctggt catgccctgg aaggcagtgg ctgagattgc tggcttctgg ccctttgggt
      841 ccttctgtaa catctgggtg gcctttgaca tcatgtgctc cactgcatcc atcctcaacc
      901 tctgtgtgat cagcgtggac aggtattggg ctatctccag ccctgcccgg tatgagagaa
      961 agatgacccc caaggcagcc ttcatcctga tcagtgtggc atggaccttg tctgtactca
     1021 tctccttcat cccagtgcag ctcagctggc acaaggcaaa acccacaagc ccctctgatg
     1081 gaaatgccac ttccctggct gagaccatag acaactgtga ctccagcctc agcaggacat
     1141 atgccatctc atcctctgta atcagctttt acatccctgt ggccatcatg attgtcacct
     1201 acaccaggat ctacaggatt gctcagaaac agctgagctc actcattaac gtctatatca
     1261 aggccgacaa gcagaagaac ggcatcaagg cgaacttcaa gatccgccac aacatcgagg
     1321 acggcggcgt gcagctcgcc taccactacc agcagaacac ccccatcggc gacggccccg
     1381 tgctgctgcc cgacaaccac tacctgagcg tgcagtccaa actttcgaaa gaccccaacg
     1441 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     1501 tggacgagct gtacaagggc ggtaccggag ggagcatggt gagcaagggc gaggagctgt
     1561 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     1621 gcgtgtccgg cgagggtgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     1681 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
     1741 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     1801 tgcccgaagg ctacatccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     1861 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     1921 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac aatcatgacc
     1981 aactgaaaag agaaactaaa gtcctgaaga ctctgtcggt gatcatgggt gtgtttgtgt
     2041 gctgttggct acctttcttc atcttgaact gcattttgcc cttctgtggg tctggggaga
     2101 cgcagccctt ctgcattgat tccaacacct ttgacgtgtt tgtgtggttt gggtgggcta
     2161 attcatcctt gaaccccatc atttatgcct ttaatgctga ttttcggaag gcattttcaa
     2221 ccctcttagg atgctacaga ctttgccctg cgacgaataa tgccatagag acggtgagta
     2281 tcaataacaa tggggccgcg atgttttcca gccatcatga gccacgaggc tccatctcca
     2341 aggagtgcaa tctggtttac ctgatcccac atgctgtggg ctcctctgag gacctgaaaa
     2401 aggaggaggc agctggcatc gccagaccct tggagaagct gtccccagcc ctatcggtca
     2461 tattggacta tgacactgac gtctctctgg agaagatcca acccatcaca caaaacggtc
     2521 agcacccaac ctgaaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat
     2581 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     2641 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     2701 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     2761 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     2821 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     2881 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     2941 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     3001 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     3061 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     3121 gggccgcctc cccgcatcga taccgtcgac ctcgacccgg gcggccgctt cgagcagaca
     3181 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     3241 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     3301 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag atgtgggagg
     3361 ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat cttcctagag
     3421 catggctacg tagataagta gcatggcggg ttaatcatta actacaagga acccctagtg
     3481 atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag
     3541 gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc
     3601 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     3661 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     3721 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     3781 caaaaggcca gcaaaaagcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     3841 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     3901 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     3961 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     4021 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     4081 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     4141 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     4201 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     4261 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     4321 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     4381 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     4441 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     4501 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     4561 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     4621 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     4681 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     4741 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     4801 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     4861 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     4921 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     4981 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     5041 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     5101 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     5161 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     5221 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     5281 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     5341 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     5401 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     5461 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     5521 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     5581 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     5641 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     5701 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     5761 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     5821 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     5881 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     5941 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     6001 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
     6061 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tgcgcgctcg
     6121 ctcgctcact gaggccgccc gggcaaagcc cgggcgtcgg gcgacctttg gtcgcccggc
     6181 ctcagtgagc gagcgagcgc gcagagaggg agtggccaac tccatcacta ggggttcctt
     6241 gtagttaatg attaacccgc catgctactt atctacgtag ccatgctcta ggaagatctc
     6301 tgcagagggc cctgcgtatg
//
