LOCUS       sequence-27085-e         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Raucher D, Stauffer T, Chen W, Shen K, Guo S, York JD, Sheetz MP, 
            Meyer T
  TITLE     Phosphatidylinositol 4,5-bisphosphate functions as a second 
            messenger that regulates cytoskeleton-plasma membrane adhesion.
  JOURNAL   Cell. 2000 Jan 21. 100(2):221-8.
  PUBMED    10660045
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2000 
            Jan 21. 100(2):221-8."
COMMENT     Sequence Label: GFP-C1-AKT-PH
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    26..91
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    654..775
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(691..710)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     745..764
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tctcggcatg gacgagctgt acaagtccgg actcagatct cgagctcaag cttcgaattc
       61 tgcagtcgac ggtaccgcgg gcccgggatc caacgacgta gccattgtga aggagggctg
      121 gctgcacaaa cgaggggaat atattaaaac ctggcggcca cgctacttcc tcctcaagaa
      181 cgatggcacc tttattggct acaaggaacg gcctcaggat gtggatcagc gagagtcccc
      241 actcaacaac ttctcagtgg cacaatgcca gctgatgaag acagagcggc caaggcccaa
      301 cacctttatc atccgctgcc tgcagtggac cacagtcatt gagcgcacct tccatgtgga
      361 aacgcctgag gagcgggaag aatgggccac cgccattcag actgtggccg atggactcaa
      421 gaggcaggaa gaagagacga tggacttccg atcaggctca cccagtgaca actcaggggc
      481 tgaagagatg gaggtgtccc tggccaagcc caagcaccgt gtgaccatgg aattcatcta
      541 gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
      601 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
      661 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
      721 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaacgcg
      781 taaattgtaa gc
//
