LOCUS       sequence-270959-        5720 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcriptional mcherry reporter of acr-2 (cholinergic) expressing 
            neurons in C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5720)
  AUTHORS   Jospin M, Qi YB, Stawicki TM, Boulin T, Schuske KR, Horvitz HR, 
            Bessereau JL, Jorgensen EM, Jin Y
  TITLE     A neuronal acetylcholine receptor regulates the balance of muscle 
            excitation and inhibition in Caenorhabditis elegans.
  JOURNAL   PLoS Biol. 2009 Dec;7(12):e1000265. doi: 
            10.1371/journal.pbio.1000265. Epub 2009 Dec 22.
  PUBMED    20027209
REFERENCE   2  (bases 1 to 5720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2009-12"; volume: "7(12):e1000265. doi"; pages: " 
            10.1371/journal.pbio.1000265. Epub 2009 Dec 22"
COMMENT     Sequence Label: pCZGY847
FEATURES             Location/Qualifiers
     source          1..5720
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1259..1281)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1381..1400
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1482..1500)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1568..1672
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1673..1741,1742..2533)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1891..1910)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2704..3292
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3193..3212
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3446..3463
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3580..3601
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3616..3621,3622..3639,3640..3646)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3654..3670
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3659..3681
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3678..3694
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3678..3694
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3726..3742
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3727..3743
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3758..3782
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(join(5710..5720,1..14))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtacaaag tggtgatatc gaattcctgc agcccggggg atccactagt ctccatggtc
       61 tcaaagggtg aagaagataa catggcaatt attaaagagt ttatgcgttt caaggtgcat
      121 atggagggat ctgtcaatgg gcatgagttt gaaattgaag gtgaaggaga aggccgacca
      181 tatgagggaa cacaaaccgc aaaactaaag gtaagtttaa acatatatat actaactaac
      241 cctgattatt taaattttca ggtaactaaa ggcggaccat taccattcgc ctgggacatc
      301 ctctctccac agttcatgta tggaagtaaa gcttatgtta aacatccggc agatatacca
      361 gattatttga aactttcatt cccggagggt tttaagtggg aacgcgtaat gaattttgaa
      421 gacggaggag ttgttacagt gacgcaagac tcaaggtaag tttaaacagt tcggtactaa
      481 ctaaccatac atatttaaat tttcagcctc caagatggag aatttattta taaagtcaaa
      541 cttcgaggaa cgaatttccc ctcggatgga cctgttatgc agaagaagac tatgggatgg
      601 gaagcttcaa gtgaaagaat gtaccctgaa gacggtgctc ttaagggaga gattaaacaa
      661 cgtcttaaat tgaaagatgg aggacattac gatgctgagg taagtttaaa catgatttta
      721 ctaactaact aatctgattt aaattttcag gtgaagacaa cttacaaagc caaaaaacca
      781 gttcagctgc caggagcgta caatgttaat attaaactgg atatcacctc ccacaacgag
      841 gattacacta tcgttgagca atatgaaaga gctgaagggc ggcactcgac aggtggcatg
      901 gatgaattgt ataaggctag cggtggagca taatgaccgc ggataacaaa tttcatatgt
      961 ttttgtttgt tttttgtctt aattaccaca ggtcatttct ctctttctat cgtcattttc
     1021 ttgtcatttt ttctttcttg agcattggca acaaatttat tcgttatgca ttatgggtac
     1081 ttgtttctcg ctttttaatg tataatctca attcttgaac taaaactctc agtgcttctg
     1141 aaaattatgg ttacaatgaa ttagtttatt aaatttatta tttgaattaa taccgaaaca
     1201 tttaaatatt aacgccaggg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
     1261 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     1321 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
     1381 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     1441 gcgtaaggag aaaataccgc atcaggcggc cttaattaag ggcctcgtga tacgcctatt
     1501 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     1561 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     1621 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     1681 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     1741 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     1801 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     1861 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     1921 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     1981 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     2041 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     2101 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     2161 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     2221 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     2281 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     2341 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     2401 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     2461 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     2521 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     2581 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     2641 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     2701 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     2761 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     2821 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     2881 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     2941 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3001 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3061 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     3121 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     3181 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     3241 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     3301 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     3361 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     3421 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     3481 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     3541 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     3601 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     3661 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc ttgcatgcac
     3721 tagactcgag gtcgacggta tcgataagct tgatatcaca agtttgtaca aaaaagcagg
     3781 ctccgaattg ggatccggta ccgttttcag ctcaccaccg actgattttc atttcaaaat
     3841 taatgcagat ttctacgtgt tctttttcaa ataaatttat atattttttg ttctctcaca
     3901 aaataaataa tttcgagagt taagtgatca ttctcagaca tattctataa ttgtccgtca
     3961 ggaaacagat agaggaacct ttgataaata aattataaaa ggccgacagg gaaatgcttc
     4021 tccctcgact ctgttagaag cgaagaggca atttttaaaa atttcccatt tttatgcgtt
     4081 tttctgattt ccaaagtcac gcatcattta ccaaccgcaa cgattcataa ggacgtaatt
     4141 ttgattttct ggacgatttt tatatagttt tgatgtgaaa ttcgctttat ttgtctgaaa
     4201 gtggaataga tttagtgtgg atttaaccga aaaatgcaga tcttttaaaa atttaaacca
     4261 gtgttttgtt gccttatttg cgaaaaaaaa ttgtgatatt tcgagtattg ctgcaatttg
     4321 attagtaaaa tgttaggata taagtattta aaaagtataa ttggttgaat tttctttttc
     4381 atcaccgact tccaaaaata tagagatact gagttttcac cattttttga atgtaaataa
     4441 aaacattttg aacacttgtg ctgggattca atccaaatac attcactgtc atttaatatt
     4501 attatatatc aaaataaaac gcataacttt ttctgttttt ttttaaattt tttcaaaatg
     4561 taggtttggt aagttaaggt aaggttaggc aatgtaggta aacacgagat gatactccac
     4621 gttgaaaatt gttttcataa atacagttta gattgaactg aaggatttta tcgtctaata
     4681 gaatataatt ttttagttca agatttagga tttgttttca ctttttttta gattcaagac
     4741 atatatctcg caaaatgagt tttgtctttg agccatcttg tcatcatagc acattgaatt
     4801 ttgttgttcg caagacggaa tatatgttcc cagctgcaaa ttttgtgtcg gatatttctt
     4861 ctttagcgaa aggcgttaaa aaccaaaaat agtcgaagcg tttgatatgt gtcccgttgg
     4921 tttttcgtgt ccatctattc tgtttcgagt acacatgttg cgacgcggca ctgatgaaaa
     4981 agtgaaagag ctagtgtcac acacaaacac aaagtatgct catgtgagtg tgtagatatg
     5041 tgtagatgta tgtcctgaag gaataaaaag actttttgaa gctacatttt taaacaaaaa
     5101 aagacagaaa catgaagcac aactctctat tctgtcgaac ggcgtgtgca actcacgcac
     5161 acccaaaaat atgaaatata acattcgcac caacaaagcg cgaatatcaa tgaatctcga
     5221 gggaacgatg gctgcctgcc ggcgcgtgtt gctcttctgt cattgggata actggcgggt
     5281 ctgttgggtc cgtcctttag accatttgac tacactgccc tgcatatgtg tgaaacgtcg
     5341 tcgttgctgt tgggagctgt ctgtgtgtat gtgtgtgtgt gtgagtatgg agcagtaaaa
     5401 agctgccgat acgagaagcg ccgccccgga cggacggcga tggcgcatcg ccaaatgtgt
     5461 cattttagtt gtcttcacga accagagggc ccacgacgga cgggcggcca cgcccacctc
     5521 aaccagaata tccttccttc tcccacccac ccttgcggac gcgcggatgc gctgaacaaa
     5581 aagacgacga agaagaagaa gaagaagctg aacgaagaaa aaagcatcaa aagaagtgaa
     5641 gcggctctac caccccgacc accttccttc acaggaagga cgccgttttc aagcttacta
     5701 gtgaattcga cccagctttc
//
