LOCUS       sequence-27100-e         643 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Bruderer RM, Brasseur C, Meyer HH
  TITLE     The AAA ATPase p97/VCP interacts with its alternative co-factors, 
            Ufd1-Npl4 and p47, through a common bipartite binding mechanism.
  JOURNAL   J Biol Chem. 2004 Nov 26. 279(48):49609-16.
  PUBMED    15371428
REFERENCE   2  (bases 1 to 643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2004 Nov 26. 279(48):49609-16."
COMMENT     Sequence Label: pTrcHis-p47
FEATURES             Location/Qualifiers
     source          1..643
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             83..109
                     /codon_start=1
                     /product="synthetic cistron containing a ribosome binding 
                     site (Shine-Dalgarno sequence), for enhancing the bacterial
                     expression of a downstream cistron (Schoner, 1997)"
                     /label=minicistron
                     /note="This first cistron should terminate 3 bp upstream of
                     the ATG for the second cistron."
                     /translation="MYRLNKEE"
     CDS             125..142
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             146..178
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(182..190,191..205)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 caatttatca gacaatctgt gtgggcactc gaccggaatt atcgattaac tttattatta
       61 aaaattaaag aggtatatat taatgtatcg attaaataag gaggaataaa ccatgggggg
      121 ttctcatcat catcatcatc atggtatggc tagcatgact ggtggacagc aaatgggtcg
      181 ggatctgtac gacgatgacg ataaggatcc ggcggaggag cggcaggacg cgttgaggga
      241 gttcgtggcg gtgacgggcg ctgaggagga ccgggcccgt ttcttcttgg agtcagctgg
      301 ctgggacctg cagattgcac ttgcaagctt ttatgaggat ggaggggatg aagacattgt
      361 gaccatttca caggcaaccc caagttcagt gtccagaggc acagctccta gtgataatag
      421 agtaacatcc ttcagagacc tcattcatga ccaagatgaa gaggaagagg aagaagaggg
      481 acagaggttt tatgctgggg gctcagagag aagtggacag cagattgttg gtcctccccg
      541 gaagaaaagt cccaacgagc tggtggatga tctgtttaag ggtgccaaag agcatggggc
      601 tgtagctgtg gaacgagtga ccaagagccc tggagagacc agt
//
