LOCUS       sequence-27101-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Shen WF, Krishnan K, Lawrence HJ, Largman C
  TITLE     The HOX homeodomain proteins block CBP histone acetyltransferase 
            activity
  JOURNAL   Mol Cell Biol. 2001 Nov;21(21):7509-22
  PUBMED    11585930
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2001-11"; volume: "21(21)"; pages: "7509-2"
COMMENT     Sequence Label: HOXB6-pCMV
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        68..86
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      121..130
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             130..153
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             169..180
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cnntggngta ttagcagagc ttggttngtg aaaccgtcag atccgctagc gattacgcca
       61 agctcgaaat taaccctcac taaagggaac aaaagctgga gctccaccgc ggtggcggcc
      121 gccaccatgg attacaagga tgacgacgat aagagcccgg gcggatccat cgagggtagg
      181 ctgagctcct atttcgttaa ctccaccttc cccgtcactc tggccagcgg gcaggagtcc
      241 ttcctgggcc agctaccgct ctattcgtcg ggctatgcgg acccgctgag acattacccc
      301 gcgccctacg ggccagggcc gggccaggac aagggctttg ccacttcctc ctattacccg
      361 ccggcgggcg gtggctacgg ccgagcggcg ccctgcgact acgggccggc gccggccttc
      421 taccgcgaga aagagtcggc ctgcgcactc tccggcgccg acgagcagcc cccgttccac
      481 cccgagccgc ggaagtcgga ctgcgcgcag gacaagagcg tgttcggcga gacagaagag
      541 cagaagtgct ccactccggt ctacccgtgg atgcagcgga tgaattcgtg caacagttcc
      601 tcctttgggc ccagcggccg gcgaggccgc cagacataca cacgttacca gacgctggag
      661 ctggagaagg agtttcacta caatcgctac ctgacgcggc ggcggcgcat cganatcncc
      721 acccttgtgc ttgaagaaan ganaacgtna ttttgttcca aanccacctn antggaaaaa
      781 agaaacaatt tctcncgctc tcnctcatgc gagagagana aanagccatg agtctgaang
      841 aagagaccag gaagctnngg accggc
//
