LOCUS       sequence-27104-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Chen SX, Osipovich AB, Ustione A, Potter LA, Hipkens S, Gangula R, 
            Yuan W, Piston DW, Magnuson MA
  TITLE     Quantification of factors influencing fluorescent protein expression
            using RMCE to generate an allelic series in the ROSA26 locus in 
            mice.
  JOURNAL   Dis Model Mech. 2011 Jul;4(4):537-47. doi: 10.1242/dmm.006569. Epub 
            2011 Feb 14.
  PUBMED    21324933
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dmm.006569"; journalName: "Dis Model Mech"; date: 
            "2011-07"; volume: "4"; issue: "4"; pages: "537-47"
COMMENT     Sequence Label: pMCS.DT-A
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     44..60
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     44..60
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     intron          163..228
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 gnaatncnng ggcgaattgg agctccaccg cggtggcggc cgctctagaa ctagtggatc
       61 ccccgggctg caggaattcg atatcaagct tatcgatacc gtcgactcta gtggatctgc
      121 attccaccac tgctcccatt catcagttcc ataggttgga atctaaaata cacaaacaat
      181 tagaatcagt agtttaacac attatacact taaaaatttt atatttacct tagagcttta
      241 aatctctgta ggtagtttgt ccaattatgt cacaccacag aagtaaggtt ccttcacaaa
      301 gagatcgcct gacacgattt cctgcacagg cttgagccat atactcatac atcgcatctt
      361 ggccacgttt tccacgggtt tcaaaattaa tctcaagttc tacgcttaac gctttcgcct
      421 gttcccagtt attaatatat tcaacgctag aactcccctc agcgaaggga aggctgagca
      481 ctacacgcga agcaccatca ccgaaccttt tgataaactc ttccgttccg acttgctcca
      541 tcaacggttc agtgagactt aaacctaact ctttcttaat agtttcggca ttatccactt
      601 ttagtgcgag aaccttcgtc agtcctggat acgtcacttt gaccacgcct ccagcttttc
      661 cagagagcgg gttttcatta tctacagagt atcccgcagc gtcgtattta ttgtcggtac
      721 tataaaaccc tttccaatca tcgtcnatct cctngggaac aaattngtgt ttganacntt
      781 tnganggacc acaaaccggn taacccnggg anaaaaantt tcctcnaaaa attanaatca
      841 cactctcggn ctggnagact gagncntggg tcaggcncct nctaatggag g
//
