LOCUS       sequence-271066-         936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid with 4xMmaPylT cassette to receive genes of interest for 
            amber suppression; for transient or stable piggyBac-mediated 
            integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Meineke B, Heimgartner J, Lafranchi L, Elsasser SJ
  TITLE     Methanomethylophilus alvus Mx1201 provides basis for mutual 
            orthogonal pyrrolysyl tRNA/aminoacyl-tRNA synthetase pairs in 
            mammalian cells.
  JOURNAL   ACS Chem Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571.
  PUBMED    30260624
REFERENCE   2  (bases 1 to 936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571."
COMMENT     Sequence Label: pAS
FEATURES             Location/Qualifiers
     source          1..936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             80..348
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     intron          451..680
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     663..682
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     complement(898..915)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
       61 aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg
      121 aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg
      181 aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc
      241 gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc
      301 tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc
      361 ggcgcctact ctagagctag cgaattcgaa tttaaatcgg atccgcggcc gctggaatta
      421 attcgctgtc tgcgagggcc agctgttggg gtgagtactc cctctcaaaa gcgggcatga
      481 cttctgcgct aagattgtca gtttccaaaa acgaggagga tttgatattc acctggcccg
      541 cggtgatgcc tttgagggtg gccgcgtcca tctggtcaga aaagacaatc tttttgttgt
      601 caagcttgag gtgtggcagg cttgagatct ggccatacac ttgagtgaca atgacatcca
      661 ctttgccttt ctctccacag gtgtccactc ccaggtccaa ctgcaggtcg agcatgctcg
      721 agcggccgcg cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat
      781 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
      841 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
      901 tcgccaaagg aatgcaaggt ctgttgaatg tcgtga
//
