LOCUS       sequence-271067-         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid with 4xMmaPylT cassette and Mma PylRS for amber suppression;
            for transient or stable piggyBac-mediated integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Meineke B, Heimgartner J, Lafranchi L, Elsasser SJ
  TITLE     Methanomethylophilus alvus Mx1201 provides basis for mutual 
            orthogonal pyrrolysyl tRNA/aminoacyl-tRNA synthetase pairs in 
            mammalian cells.
  JOURNAL   ACS Chem Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571.
  PUBMED    30260624
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571."
COMMENT     Sequence Label: pAS_4xMma PylT_FLAG-Mma PylRS
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             80..348
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      380..389
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             389..412
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
       61 aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg
      121 aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg
      181 aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc
      241 gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc
      301 tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc
      361 ggcgcctact ctagagctag ccaccatgga ctacaaggac gacgacgaca agatggacaa
      421 gaagcccctg aacaccctga tcagcgccac aggactgtgg atgtccagaa ccggcaccat
      481 ccacaagatc aagcaccacg aggtgtcccg gtccaaaatc tacatcgaga tggcctgcgg
      541 cgatcacctg gtcgtcaaca acagcagaag cagccggaca gccagagccc tgcggcacca
      601 caagtacaga aagacctgca agcggtgcag agtgtccgac gaggacctga acaagttcct
      661 gaccaaggcc aacgaggacc agaccagcgt gaaagtgaag gtggtgtccg cccccacccg
      721 gaccaagaaa gccatgccca agagcgtggc cagagccccc aagcccctgg aaaacaccga
      781 agccgctcag gcccagccca gcggcagcaa gttcagcccc gccatccccg tgtctaccca
      841 ggaaagcgtc agcgtccccg ccagcgtgtc caccagcatc tctagcatct caaccggcgc
      901 cacagcttct gccctggtca agggcaacac caaccccatc accagcatgt ctgcccctgt
//
