LOCUS       sequence-271068-         602 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid with 4xMmaP cassette and Mma PylRS for ochre (TAA) 
            suppression; for transient or stable piggyBac-mediated integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Meineke B, Heimgartner J, Lafranchi L, Elsasser SJ
  TITLE     Methanomethylophilus alvus Mx1201 provides basis for mutual 
            orthogonal pyrrolysyl tRNA/aminoacyl-tRNA synthetase pairs in 
            mammalian cells.
  JOURNAL   ACS Chem Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571.
  PUBMED    30260624
REFERENCE   2  (bases 1 to 602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571."
COMMENT     Sequence Label: pAS_4xMma PylT(UUA)_FLAG-AcKRS
FEATURES             Location/Qualifiers
     source          1..602
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..58
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agagctagcg tttaaactta agcttgccac catggactac aaggacgacg acgacaagat
       61 ggacaagaag cccctgaaca ccctgatcag cgccacagga ctgtggatgt ccagaaccgg
      121 caccatccac aagatcaagc accacgaggt gtcccggtcc aaaatctaca tcgagatggc
      181 ctgcggcgat cacctggtcg tcaacaacag cagaagcagc cggacagcca gagccctgcg
      241 gcaccacaag tacagaaaga cctgcaagcg gtgcagagtg tccgacgagg acctgaacaa
      301 gttcctgacc aaggccaacg aggaccagac cagcgtgaaa gtgaaggtgg tgtccgcccc
      361 cacccggacc aagaaagcca tgcccaagag cgtggccaga gcccccaagc ccctggaaaa
      421 caccgaagcc gctcaggccc agcccagcgg cagcaagttc agccccgcca tccccgtgtc
      481 tacccaggaa agcgtcagcg tccccgccag cgtgtccacc agcatctcta gcatctcaac
      541 cggcgccaca gcttctgccc tggtcaaggg caacaccaac cccatcacca gcatgtctgc
      601 cc
//
