LOCUS       sequence-271070-         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid with 4xMx PylT cassette and MX1201 PylRS for amber 
            suppression; for transient or stable piggyBac-mediated integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Meineke B, Heimgartner J, Lafranchi L, Elsasser SJ
  TITLE     Methanomethylophilus alvus Mx1201 provides basis for mutual 
            orthogonal pyrrolysyl tRNA/aminoacyl-tRNA synthetase pairs in 
            mammalian cells.
  JOURNAL   ACS Chem Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571.
  PUBMED    30260624
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571."
COMMENT     Sequence Label: pAS_4xMx PylT_FLAG-Mx PylRS
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             191..214
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc
       61 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt
      121 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctactctaga gctagctcgc
      181 gaccaccatg gactacaagg acgacgacga caagacggta aagtatacgg atgcacagat
      241 acagcgcctt cgcgaatacg gcaacgggac ctatgagcag aaggtcttcg aggacctcgc
      301 atcgagggat gctgccttct ccaaggagat gtccgtcgcc tctaccgaca acgagaagaa
      361 gatcaagggg atgatcgcca atccgtcccg tcatggattg acccagctga tgaacgacat
      421 cgccgacgca ttggtcgccg agggtttcat cgaggtccgt acgcccatat tcatatcgaa
      481 ggatgcgctg gcacgtatga ccatcaccga ggacaagccc cttttcaagc aggtcttctg
      541 gatcgacgag aaaagggcgc tcaggcctat gctggcacct aacctttatt ccgtcatgag
      601 ggacctgagg gaccatacgg acggtccggt gaagatcttc gagatgggtt cctgcttcag
      661 gaaggagtcc cacagcggga tgcatctgga ggagttcacc atgctgaacc tcgtggacat
      721 gggtccccgc ggagacgcca cggaggtcct gaagaactac atatcggtcg tgatgaaggc
      781 ggccggtctc ccggactacg acctcgtaca ggaggagtcc gacgt
//
