LOCUS       sequence-271071-         659 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid with 4xMx PylT cassette and MX1201 PylRS for amber 
            suppression; for transient or stable piggyBac-mediated integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Meineke B, Heimgartner J, Lafranchi L, Elsasser SJ
  TITLE     Methanomethylophilus alvus Mx1201 provides basis for mutual 
            orthogonal pyrrolysyl tRNA/aminoacyl-tRNA synthetase pairs in 
            mammalian cells.
  JOURNAL   ACS Chem Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571.
  PUBMED    30260624
REFERENCE   2  (bases 1 to 659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571."
COMMENT     Sequence Label: pAS_4xMx PylT_FLAG-Mx PylRS
FEATURES             Location/Qualifiers
     source          1..659
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gcagatcctt tctagagcac tagtggcctt ggagctgcag tatttagcat gccccaccca
       61 tctgcaaggc attctggata gtgtcaaaac agccggaaat caagtccgtt tatctcaaac
      121 tttagcattt tgggaataaa tgatatttgc tatgctggtt aaattagatt ttagttaaat
      181 ttcctgctga agctctagta cgataagtaa cttgacctaa gtgtaaagtt gagatttcct
      241 tcaggtttat atagcttgtg cgccgcctgg gtacctcggg ggacggtccg gcgaccagcg
      301 ggtctctaaa acctagccag cggggttcga cgccccggtc tctcggacaa gtgcgggttt
      361 ttatcgcctg cagtatttag catggcccca cccatctgca aggcattctt ggatagtgtc
      421 aaaacagccg gaaatcaagt ccgtttatct caaactttag cattttggga ataaatgata
      481 tttgctatgc tggttaaatt agattttagt taaatttcct gctgaagctc tagtacgata
      541 agtaacttga cctaagtgta aagttgagat ttccttcagg tttatatagc ttgtgcgccg
      601 cctgggtacc tcgggggacg gtccggcgac cagcgggtct ctaaaaccta gccagcggg
//
