LOCUS       sequence-271072-         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid with 4xMx PylT cassette and MX1201 PylRS for amber 
            suppression; for transient or stable piggyBac-mediated integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Meineke B, Heimgartner J, Lafranchi L, Elsasser SJ
  TITLE     Methanomethylophilus alvus Mx1201 provides basis for mutual 
            orthogonal pyrrolysyl tRNA/aminoacyl-tRNA synthetase pairs in 
            mammalian cells.
  JOURNAL   ACS Chem Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571.
  PUBMED    30260624
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571."
COMMENT     Sequence Label: pAS_4xMx PylT_FLAG-Mx PylRS YA
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             243..266
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct
       61 caggtcgaga ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct
      121 ctccacgctt tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct
      181 gcgccgttac agatccaagc tgtgaccggc gcctactcta gagctagctc gcgaccacca
      241 tggactacaa ggacgacgac gacaagacgg taaagtatac ggatgcacag atacagcgcc
      301 ttcgcgaata cggcaacggg acctatgagc agaaggtctt cgaggacctc gcatcgaggg
      361 atgctgcctt ctccaaggag atgtccgtcg cctctaccga caacgagaag aagatcaagg
      421 ggatgatcgc caatccgtcc cgtcatggat tgacccagct gatgaacgac atcgccgacg
      481 cattggtcgc cgagggtttc atcgaggtcc gtacgcccat attcatatcg aaggatgcgc
      541 tggcacgtat gaccatcacc gaggacaagc cccttttcaa gcaggtcttc tggatcgacg
      601 agaaaagggc gctcaggcct atgctggcac ctaaccttgc ctccgtcatg agggacctga
      661 gggaccatac ggacggtccg gtgaagatct tcgagatggg ttcctgcttc aggaaggagt
      721 cccacagcgg gatgcatctg gaggagttca ccatgctgaa cctcgtggac atgggtcccc
      781 gcggagacgc cacggaggtc ctgaagaact acatatcggt cgtgatgaag gcggccggtc
      841 tcccg
//
