LOCUS       sequence-271073-         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid with 4xMx PylT cassette and MX1201 PylRS for amber 
            suppression; for transient or stable piggyBac-mediated integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Meineke B, Heimgartner J, Lafranchi L, Elsasser SJ
  TITLE     Methanomethylophilus alvus Mx1201 provides basis for mutual 
            orthogonal pyrrolysyl tRNA/aminoacyl-tRNA synthetase pairs in 
            mammalian cells.
  JOURNAL   ACS Chem Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571.
  PUBMED    30260624
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571."
COMMENT     Sequence Label: pAS_4xMx PylT_FLAG-Mx PylRS YA
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tctgcaaggc attctggata gtgtcaaaac agccggaaat caagtccgtt tatctcaaac
       61 tttagcattt tgggaataaa tgatatttgc tatgctggtt aaattagatt ttagttaaat
      121 ttcctgctga agctctagta cgataagtaa cttgacctaa gtgtaaagtt gagatttcct
      181 tcaggtttat atagcttgtg cgccgcctgg gtacctcggg ggacggtccg gcgaccagcg
      241 ggtctctaaa acctagccag cggggttcga cgccccggtc tctcggacaa gtgcggtttt
      301 tcttgctgca gtatttagca tgccccaccc atctgcaagg cattctggat agtgtcaaaa
      361 cagccggaaa tcaagtccgt ttatctcaaa ctttagcatt ttgggaataa atgatatttg
      421 ctatgctggt taaattagat tttagttaaa tttcctgctg aagctctagt acgataagta
      481 acttgaccta agtgtaaagt tgagatttcc ttcaggttta tatagcttgt gcgccgcctg
      541 ggtacctcgg gggacggtcc ggcgaccagc gggtctctaa aacctagcca gcggggttcg
      601 acgccccggt ctctcggaca agtgcggttt ttttgcctgc agtatttagc atgccccacc
      661 catctgcaag gcattctgga tagtgtcaaa acagccggaa atcaagtccg tttatctcaa
      721 actttagcat tttgggaata aatgatattt gctatgctgg ttaaattaga ttttagttaa
      781 atttcctgct gaagctctag tacgataagt aacttgacct aagtgtaaag ttgagatttc
      841 cttcaggttt atatagcttg tgcgccgcct gggtacctcg ggggacggtc cggcgaccag
      901 cgggtctcta aaacctagcc agcggggttc gacgccccgg tctctcggac aagtgcgg
//
