LOCUS       sequence-271074-         645 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid with 4xMx PylT C41CA cassette and MX1201 PylRS for amber 
            suppression; for transient or stable piggyBac-mediated integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Meineke B, Heimgartner J, Lafranchi L, Elsasser SJ
  TITLE     Methanomethylophilus alvus Mx1201 provides basis for mutual 
            orthogonal pyrrolysyl tRNA/aminoacyl-tRNA synthetase pairs in 
            mammalian cells.
  JOURNAL   ACS Chem Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571.
  PUBMED    30260624
REFERENCE   2  (bases 1 to 645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571."
COMMENT     Sequence Label: pAS_4xMx PylT C41CA_FLAG_Mx PylRS
FEATURES             Location/Qualifiers
     source          1..645
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             110..133
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga
       61 tccaagctgt gaccggcgcc tactctagag ctagctcgcg accaccatgg actacaagga
      121 cgacgacgac aagacggtaa agtatacgga tgcacagata cagcgccttc gcgaatacgg
      181 caacgggacc tatgagcaga aggtcttcga ggacctcgca tcgagggatg ctgccttctc
      241 caaggagatg tccgtcgcct ctaccgacaa cgagaagaag atcaagggga tgatcgccaa
      301 tccgtcccgt catggattga cccagctgat gaacgacatc gccgacgcat tggtcgccga
      361 gggtttcatc gaggtccgta cgcccatatt catatcgaag gatgcgctgg cacgtatgac
      421 catcaccgag gacaagcccc ttttcaagca ggtcttctgg atcgacgaga aaagggcgct
      481 caggcctatg ctggcaccta acctttattc cgtcatgagg gacctgaggg accatacgga
      541 cggtccggtg aagatcttcg agatgggttc ctgcttcagg aaggagtccc acagcgggat
      601 gcatctggag gagttcacca tgctgaacct cgtggacatg ggtcc
//
