LOCUS       sequence-271075-         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid with 4xMx PylT C41CA cassette and MX1201 PylRS for amber 
            suppression; for transient or stable piggyBac-mediated integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Meineke B, Heimgartner J, Lafranchi L, Elsasser SJ
  TITLE     Methanomethylophilus alvus Mx1201 provides basis for mutual 
            orthogonal pyrrolysyl tRNA/aminoacyl-tRNA synthetase pairs in 
            mammalian cells.
  JOURNAL   ACS Chem Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571.
  PUBMED    30260624
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571."
COMMENT     Sequence Label: pAS_4xMx PylT C41CA_FLAG_Mx PylRS
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cgcagatcct ttctagagca ctagtggcct tggagctgca gtatttagca tgccccaccc
       61 atctgcaagg cattctggat agtgtcaaaa cagccggaaa tcaagtccgt ttatctcaaa
      121 ctttagcatt ttgggaataa atgatatttg ctatgctggt taaattagat tttagttaaa
      181 tttcctgctg aagctctagt acgataagta acttgaccta agtgtaaagt tgagatttcc
      241 ttcaggttta tatagcttgt gcgccgcctg ggtacctcgg gggacggtcc ggcgaccagc
      301 gggtctctaa aacctagcca agcggggttc gacgccccgg tctctcggac aagtgcggtt
      361 tttatcgctg cagtatttag catgccccac ccatctgcaa ggcattctgg atagtgtcaa
      421 aacagccgga aatcaagtcc gtttatctca aactttagca ttttgggaat aaatgatatt
      481 tgctatgctg gttaaattag attttagtta aatttcctgc tgaagctcta gtacgataag
      541 taacttgacc taagtgtaaa gttgagattt ccttcaggtt tatatagctt gtgcgccgcc
      601 tgggtacctc gggggacggt ccggcgacca gcgggtctct aaaacctagc caagcggggt
      661 tcgacgcccc ggtctctcgg acaagtgcgg tttttcttgc tgcagtattt agcatgcccc
      721 acccatctgc aaggcattct ggatagtgtc aaaacagccg gaaatcaagt ccgtttatct
      781 caaactttag cattttggga ataaatgata tttgctatgc tggttaaatt agattttagt
      841 taaatttcct gctgaagctc tagtacgata agtaacttga cctaagtgta aagttgagat
      901 ttccttcagg tttatatagc ttgtgcgccg cctgggtacc tcggg
//
