LOCUS       sequence-271076-         766 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid with 4xMx PylT C41CA cassette and MX1201 PylRS for amber 
            suppression; for transient or stable piggyBac-mediated integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Meineke B, Heimgartner J, Lafranchi L, Elsasser SJ
  TITLE     Methanomethylophilus alvus Mx1201 provides basis for mutual 
            orthogonal pyrrolysyl tRNA/aminoacyl-tRNA synthetase pairs in 
            mammalian cells.
  JOURNAL   ACS Chem Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571.
  PUBMED    30260624
REFERENCE   2  (bases 1 to 766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571."
COMMENT     Sequence Label: pAS_4xMx PylT C41CA_FLAG_Mx PylRS YA
FEATURES             Location/Qualifiers
     source          1..766
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             162..185
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tccggcgctc ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct
       61 gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct
      121 gtgaccggcg cctactctag agctagctcg cgaccaccat ggactacaag gacgacgacg
      181 acaagacggt aaagtatacg gatgcacaga tacagcgcct tcgcgaatac ggcaacggga
      241 cctatgagca gaaggtcttc gaggacctcg catcgaggga tgctgccttc tccaaggaga
      301 tgtccgtcgc ctctaccgac aacgagaaga agatcaaggg gatgatcgcc aatccgtccc
      361 gtcatggatt gacccagctg atgaacgaca tcgccgacgc attggtcgcc gagggtttca
      421 tcgaggtccg tacgcccata ttcatatcga aggatgcgct ggcacgtatg accatcaccg
      481 aggacaagcc ccttttcaag caggtcttct ggatcgacga gaaaagggcg ctcaggccta
      541 tgctggcacc taaccttgcc tccgtcatga gggacctgag ggaccatacg gacggtccgg
      601 tgaagatctt cgagatgggt tcctgcttca ggaaggagtc ccacagcggg atgcatctgg
      661 aggagttcac catgctgaac ctcgtggaca tgggtccccg cggagacgcc acggaggtcc
      721 tgaagaacta catatcggtc gtgatgaagg cggccggtct cccgga
//
