LOCUS       sequence-271077-         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid with 4xMx PylT C41CA cassette and MX1201 PylRS for amber 
            suppression; for transient or stable piggyBac-mediated integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Meineke B, Heimgartner J, Lafranchi L, Elsasser SJ
  TITLE     Methanomethylophilus alvus Mx1201 provides basis for mutual 
            orthogonal pyrrolysyl tRNA/aminoacyl-tRNA synthetase pairs in 
            mammalian cells.
  JOURNAL   ACS Chem Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571.
  PUBMED    30260624
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571."
COMMENT     Sequence Label: pAS_4xMx PylT C41CA_FLAG_Mx PylRS YA
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tttctagagc actagtggcc ttggagctgc agtatttagc atgccccacc catctgcaag
       61 gcattctgga tagtgtcaaa acagccggaa atcaagtccg tttatctcaa actttagcat
      121 tttgggaata aatgatattt gctatgctgg ttaaattaga ttttagttaa atttcctgct
      181 gaagctctag tacgataagt aacttgacct aagtgtaaag ttgagatttc cttcaggttt
      241 atatagcttg tgcgccgcct gggtacctcg ggggacggtc cggcgaccag cgggtctcta
      301 aaacctagcc aagcggggtt cgacgccccg gtctctcgga caagtgcggt ttttatcgct
      361 gcagtattta gcatgcccca cccatctgca aggcattctg gatagtgtca aaacagccgg
      421 aaatcaagtc cgtttatctc aaactttagc attttgggaa taaatgatat ttgctatgct
      481 ggttaaatta gattttagtt aaatttcctg ctgaagctct agtacgataa gtaacttgac
      541 ctaagtgtaa agttgagatt tccttcaggt ttatatagct tgtgcgccgc ctgggtacct
      601 cgggggacgg tccggcgacc agcgggtctc taaaacctag ccaagcgggg ttcgacgccc
      661 cggtctctcg gacaagtgcg gtttttcttg ctgcagtatt tagcatgccc cacccatctg
      721 caaggcattc tggatagtgt caaaacagcc ggaaatcaag tccgtttatc tcaaacttta
      781 gcattttggg aataaatgat atttgctatg ctggttaaat tagattttag ttaaatttcc
      841 tgctgaagct ctagtacgat aagtaacttg acctaagtgt aaagttgaga tttccttcag
      901 gtttatatag cttgtgcgcc gcctgggtac ctcgggggac ggtccggcga ccagc
//
