LOCUS       sequence-271079-         694 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid with 4xMxPylT C41CA cassette and amber suppression reporter 
            sfGFP 150 TAG stop; for transient transfection or stable 
            piggyBac-mediated integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Meineke B, Heimgartner J, Lafranchi L, Elsasser SJ
  TITLE     Methanomethylophilus alvus Mx1201 provides basis for mutual 
            orthogonal pyrrolysyl tRNA/aminoacyl-tRNA synthetase pairs in 
            mammalian cells.
  JOURNAL   ACS Chem Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571.
  PUBMED    30260624
REFERENCE   2  (bases 1 to 694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571."
COMMENT     Sequence Label: pAS_4xMx PylT C41CA_GFP 150TAG
FEATURES             Location/Qualifiers
     source          1..694
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      116..125
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt
       61 ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tactctagag ctagcgccac
      121 catggtgtcc aagggcgagg aactgttcac cggcgtggtg cccatcctgg tggaactgga
      181 tggcgacgtg aacggccaca agttctctgt gcggggagag ggcgaaggcg acgccacaaa
      241 tggcaagctg accctggagt tcatctgcac caccggcaag ctgcccgtgc cttggcctac
      301 cctcgtgacc acactgacct acggcgtgca gtgcttcagc agataccccg accatatgaa
      361 gcggcacgac ttcttcaaga gcgccatgcc cgagggctac gtgcaggaac ggaccatcag
      421 cttcaaggac gacggcacct acaagaccag agccgaagtg aagttcgagg gcgacaccct
      481 cgtgaaccgg atcgagctga agggcatcga tttcaaagag gacggcaaca tcctgggcca
      541 caagctggag tacaacttca acagccacta ggtgtacatc accgccgaca agcagaagaa
      601 cggcatcaag gccaacttca agatccggca caacgtggaa gatggcagcg tgcagctggc
      661 cgaccactac cagcagaaca cccccatcgg agat
//
