LOCUS       sequence-271081-         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid with 4xBstTyrT(CUA) cassette and E. coli TyrRS for amber 
            suppression; for transient or stable piggyBac-mediated integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Meineke B, Heimgartner J, Caridha R, Block MF, Kimler KJ, Pires MF, 
            Landreh M, Elsasser SJ
  TITLE     Dual stop codon suppression in mammalian cells with genomically 
            integrated genetic code expansion machinery.
  JOURNAL   Cell Rep Methods. 2023 Nov 20;3(11):100626. doi: 
            10.1016/j.crmeth.2023.100626. Epub 2023 Nov 6.
  PUBMED    37935196
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Methods."; date: "2023-11-20"; volume: "3(11):100626. doi"; pages: "
            10.1016/j.crmeth.2023.100626. Epub 2023 Nov 6"
COMMENT     Sequence Label: pAS_4xBstTyrT(CUA)_EcoTyrRS-FLAG
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             643..666
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctgacccgtc gtctgcatca gaatcaggtg tttggcctga ccgttccgct gatcactaaa
       61 gcagatggca ccaaatttgg taaaactgaa ggcggcgcag tctggttgga cccgaagaaa
      121 accagcccgt acaaattcta ccagttctgg atcaacactg cggatgccga cgtttaccgc
      181 ttcctgaagt tcttcacctt tatgagcatt gaagagatca acgccctgga agaagaagat
      241 aaaaacagcg gtaaagcacc gcgcgcccag tatgtactgg cggagcaggt gactcgtctg
      301 gttcacggtg aagaaggttt acaggcggca aaacgtatta ccgaatgcct gttcagcggt
      361 tctttgagtg cgctgagtga agcggacttc gaacagctgg cgcaggacgg cgtaccgatg
      421 gttgagatgg aaaagggcgc agacctgatg caggcactgg tcgattctga actgcaacct
      481 tcccgtggtc aggcacgtaa aactatcgcc tccaatgcca tcaccattaa cggtgaaaaa
      541 cagtccgatc ctgaatactt ctttaaagaa gaagatcgtc tgtttggtcg ttttacctta
      601 ctgcgtcgcg gtaaaaagaa ttactgtctg atttgctgga aagactacaa agacgatgac
      661 gacaagtgat gaatcgatgg atccgcggcc gcgcccctct ccctcccccc cccctaacgt
      721 tactggccga agccgcttgg aataaggccg gtgtgcgttt g
//
