LOCUS       sequence-271082-         342 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid with 4xBstTyrT(CUA) cassette and E. coli TyrRS for amber 
            suppression; for transient or stable piggyBac-mediated integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Meineke B, Heimgartner J, Caridha R, Block MF, Kimler KJ, Pires MF, 
            Landreh M, Elsasser SJ
  TITLE     Dual stop codon suppression in mammalian cells with genomically 
            integrated genetic code expansion machinery.
  JOURNAL   Cell Rep Methods. 2023 Nov 20;3(11):100626. doi: 
            10.1016/j.crmeth.2023.100626. Epub 2023 Nov 6.
  PUBMED    37935196
REFERENCE   2  (bases 1 to 342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Methods."; date: "2023-11-20"; volume: "3(11):100626. doi"; pages: "
            10.1016/j.crmeth.2023.100626. Epub 2023 Nov 6"
COMMENT     Sequence Label: pAS_4xBstTyrT(CUA)_EcoTyrRS-FLAG
FEATURES             Location/Qualifiers
     source          1..342
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aaaaaccgca cttgtctgga gggggacgga ttcgaaccgc cgaacccaaa gggagcggat
       61 ttagagtccg ccgcgtttag ccacttcgct acccctccga ggtacccagg cggcgcacaa
      121 gctatataaa cctgaaggaa atctcaactt tacacttagg tcaagttact tatcgtacta
      181 gagcttcagc aggaaattta actaaaatct aatttaacca gcatagcaaa tatcatttat
      241 tcccaaaatg ctaaagtttg agataaacgg acttgatttc cggctgtttt gacactatcc
      301 agaatgcctt gcagatgggt ggggcatgct aaatactgca gc
//
