LOCUS       sequence-271083-         656 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid with 4xEcoLeuT(CUA) cassette and E. coli AnapRS for amber 
            suppression and incorporation of the fluorescent ncAA Anap; for 
            transient or stable piggyBac-mediated integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Meineke B, Heimgartner J, Caridha R, Block MF, Kimler KJ, Pires MF, 
            Landreh M, Elsasser SJ
  TITLE     Dual stop codon suppression in mammalian cells with genomically 
            integrated genetic code expansion machinery.
  JOURNAL   Cell Rep Methods. 2023 Nov 20;3(11):100626. doi: 
            10.1016/j.crmeth.2023.100626. Epub 2023 Nov 6.
  PUBMED    37935196
REFERENCE   2  (bases 1 to 656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Methods."; date: "2023-11-20"; volume: "3(11):100626. doi"; pages: "
            10.1016/j.crmeth.2023.100626. Epub 2023 Nov 6"
COMMENT     Sequence Label: pAS_4xEcoLeuT(CUA)_AnapRS
FEATURES             Location/Qualifiers
     source          1..656
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      222..231
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg
       61 agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg
      121 ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt
      181 tacagatcca agctgtgacc ggcgcctact ctagagctag cgccaccatg gaagagcaat
      241 accggcccga agagatcgag agcaaggtgc agctgcattg ggacgagaag cggaccttcg
      301 aagtgaccga ggacgagagc aaagagaagt actactgctt cagcggccct ccctacccca
      361 gcggcagact gcacatggga cacgtgcgga actacaccat cggcgacgtg atcgccagat
      421 accagcggat gctgggcaag aacgtgctgc agcccatcgg ctgggatgcc tttggactgc
      481 ctgctgaagg cgccgctgtg aagaacaaca cagcccctgc cccctggacc tacgacaata
      541 tcgcctacat gaagaaccag ctgaagatgc tgggattcgg ctacgactgg tcccgcgagc
      601 tggccacctg tacccccgag tactacagat gggagcagaa gttcttcacc gagctg
//
