LOCUS       sequence-271084-         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid with 4xEcoLeuT(CUA) cassette and E. coli AnapRS for amber 
            suppression and incorporation of the fluorescent ncAA Anap; for 
            transient or stable piggyBac-mediated integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Meineke B, Heimgartner J, Caridha R, Block MF, Kimler KJ, Pires MF, 
            Landreh M, Elsasser SJ
  TITLE     Dual stop codon suppression in mammalian cells with genomically 
            integrated genetic code expansion machinery.
  JOURNAL   Cell Rep Methods. 2023 Nov 20;3(11):100626. doi: 
            10.1016/j.crmeth.2023.100626. Epub 2023 Nov 6.
  PUBMED    37935196
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Methods."; date: "2023-11-20"; volume: "3(11):100626. doi"; pages: "
            10.1016/j.crmeth.2023.100626. Epub 2023 Nov 6"
COMMENT     Sequence Label: pAS_4xEcoLeuT(CUA)_AnapRS
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     tRNA            complement(join(197..243,244..246,247..280))
                     /product="amber suppressor variant of an E. coli leucine 
                     tRNA (Wu et al., 2004)"
                     /label=tRNA(CUA)
     tRNA            complement(join(544..590,591..593,594..627))
                     /product="amber suppressor variant of an E. coli leucine 
                     tRNA (Wu et al., 2004)"
                     /label=tRNA(CUA)
ORIGIN
        1 ttacttatcg tactagagct tcagcaggaa atttaactaa aatctaattt aaccagcata
       61 gcaaatatca tttattccca aaatgctaaa gtttgagata aacggacttg atttccggct
      121 gttttgacac tatccagaat gccttgcaga tgggtggggc atgctaaata ctgcagcaag
      181 aaaaaccgca cttgtctacc cggagcggga cttgaacccg cacagcgcga acgccgaggg
      241 atttagaatc ccttgtgtct accgattcca ccatccgggc gaggtaccca ggcggcgcac
      301 aagctatata aacctgaagg aaatctcaac tttacactta ggtcaagtta cttatcgtac
      361 tagagcttca gcaggaaatt taactaaaat ctaatttaac cagcatagca aatatcattt
      421 attcccaaaa tgctaaagtt tgagataaac ggacttgatt tccggctgtt ttgacactat
      481 ccagaatgcc ttgcagatgg gtggggcatg ctaaatactg cagcgataaa aaccgcactt
      541 gtctacccgg agcgggactt gaacccgcac agcgcgaacg ccgagggatt tagaatccct
      601 tgtgtctaca gattccacca tccgggcgag gtacccaggc ggcgcacaag ctatataaac
      661 ctgaaggaaa tctcaacttt acacttaggt caagttactt atcgtactag agcttcagca
      721 ggaaatttaa ctaaaatcta atttaaccag catagcaaat atcatttatt cccaaaatgc
      781 taaagtttga gataaacgga cttgatttcc ggctgttttg acactatcca gaatgccttg
      841 cagatgggtg gggcatgcta aatactgcag ctccaaggc
//
