LOCUS       sequence-271086-         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid with 4xMmaPylT cassette and chimeric Mma/Mba PylRS for amber
            suppression and incorporation of photo-crosslinking-lysine; for 
            transient or stable piggyBac-mediated integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Meineke B, Heimgartner J, Caridha R, Block MF, Kimler KJ, Pires MF, 
            Landreh M, Elsasser SJ
  TITLE     Dual stop codon suppression in mammalian cells with genomically 
            integrated genetic code expansion machinery.
  JOURNAL   Cell Rep Methods. 2023 Nov 20;3(11):100626. doi: 
            10.1016/j.crmeth.2023.100626. Epub 2023 Nov 6.
  PUBMED    37935196
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Methods."; date: "2023-11-20"; volume: "3(11):100626. doi"; pages: "
            10.1016/j.crmeth.2023.100626. Epub 2023 Nov 6"
COMMENT     Sequence Label: pAS_4xMma PylT_FLAG-AbKRS-chIPYE
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aaccccggga atctaacccg gctgaacgga tttagagtcc gttcgatcta catgatcagg
       61 tttccgaggt acccaggcgg cgcacaagct atataaacct gaaggaaatc tcaactttac
      121 acttaggtca agttacttat cgtactagag cttcagcagg aaatttaact aaaatctaat
      181 ttaaccagca tagcaaatat catttattcc caaaatgcta aagtttgaga taaacggact
      241 tgatttccgg ctgttttgac actatccaga atgccttgca gatgggtggg gcatgctaaa
      301 tactgcagga aaaaccgcac ttgtccggaa accccgggaa tctaacccgg ctgaacggat
      361 ttagagtccg ttcgatctac atgatcaggt ttccgaggta cccaggcggc gcacaagcta
      421 tataaacctg aaggaaatct caactttaca cttaggtcaa gttacttatc gtactagagc
      481 ttcagcagga aatttaacta aaatctaatt taaccagcat agcaaatatc atttattccc
      541 aaaatgctaa agtttgagat aaacggactt gatttccggc tgttttgaca ctatccagaa
      601 tgccttgcag atgggtgggg catgctaaat actgcaggaa aaaccgcact tgtccggaaa
      661 ccccgggaat ctaacccggc tgaacggatt tagagtccgt tcgatctaca tgatcaggtt
      721 tccgaggtac ccaggcggcg cacaagctat ataaacctga aggaaatctc aactttacac
      781 ttaggtcaag ttacttatcg tactagagct tcagcaggaa atttaactaa aatctaattt
      841 aaccagcata gcaaatatca tttattccca aaatgctaaa gtttgagata aacggacttg
      901 atttccggct gttttgacac tatccagaat gccttgcaga tgggtggggc atgctaaata
      961 ctgcagaact agtgctctag aaaggatctg
//
