LOCUS       sequence-271087-         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression 
            and incorporation of acetyl-lysine; for transient or stable 
            piggyBac-mediated integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Meineke B, Heimgartner J, Caridha R, Block MF, Kimler KJ, Pires MF, 
            Landreh M, Elsasser SJ
  TITLE     Dual stop codon suppression in mammalian cells with genomically 
            integrated genetic code expansion machinery.
  JOURNAL   Cell Rep Methods. 2023 Nov 20;3(11):100626. doi: 
            10.1016/j.crmeth.2023.100626. Epub 2023 Nov 6.
  PUBMED    37935196
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Methods."; date: "2023-11-20"; volume: "3(11):100626. doi"; pages: "
            10.1016/j.crmeth.2023.100626. Epub 2023 Nov 6"
COMMENT     Sequence Label: pAS_4xMma PylT_FLAG-AcKRS
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      225..234
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             234..257
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt
       61 gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc
      121 tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac agatccaagc
      181 tgtgaccggc gcctactcta gagctagcgt ttaaacttaa gcttgccacc atggactaca
      241 aggacgacga cgacaagatg gacaagaagc ccctgaacac cctgatcagc gccacaggac
      301 tgtggatgtc cagaaccggc accatccaca agatcaagca ccacgaggtg tcccggtcca
      361 aaatctacat cgagatggcc tgcggcgatc acctggtcgt caacaacagc agaagcagcc
      421 ggacagccag agccctgcgg caccacaagt acagaaagac ctgcaagcgg tgcagagtgt
      481 ccgacgagga cctgaacaag ttcctgacca aggccaacga ggaccagacc agcgtgaaag
      541 tgaaggtggt gtccgccccc acccggacca agaaagccat gcccaagagc gtggccagag
      601 cccccaagcc cctggaaaac accgaagccg ctcaggccca gcccagcggc agcaagttca
      661 gccccgccat ccccgtgtct acccaggaaa gcgtcagcgt ccccgccagc gtgtccacca
      721 gcatctctag catctcaacc ggcgccacag cttctgccct ggtcaagggc aacaccaacc
      781 ccatcaccag catgtctgcc cc
//
