LOCUS       sequence-271089-         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid with 4xMmaPylT cassette and MmaPylRS AF for amber 
            suppression; for transient or stable piggyBac-mediated integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Meineke B, Heimgartner J, Lafranchi L, Elsasser SJ
  TITLE     Methanomethylophilus alvus Mx1201 provides basis for mutual 
            orthogonal pyrrolysyl tRNA/aminoacyl-tRNA synthetase pairs in 
            mammalian cells.
  JOURNAL   ACS Chem Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571.
  PUBMED    30260624
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571."
COMMENT     Sequence Label: pAS_4xMma PylT_FLAG-Mma PylRS AF
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      166..175
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             175..198
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
       61 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
      121 cgccgttaca gatccaagct gtgaccggcg cctactctag agctagccac catggactac
      181 aaggacgacg acgacaagat ggacaaaaaa ccgctgaata ccctgatctc tgctactggt
      241 ctgtggatga gtcgtaccgg aaccattcat aaaatcaaac accacgaggt tagccgttcg
      301 aaaatctata ttgagatggc gtgtggcgat catctggttg tgaacaatag ccgctcttct
      361 cgtacagcac gtgcactgcg tcaccacaaa tatcgtaaaa cctgtaaacg ttgccgtgtg
      421 tccgatgagg atctgaacaa attcctgaca aaagccaatg aggaccaaac aagcgtgaaa
      481 gtgaaagtcg ttagcgctcc tacccgtact aaaaaagcaa tgccgaaatc cgttgctcgt
      541 gcccctaaac cactggaaaa cactgaagca gcacaggcac agccgtctgg aagcaaattc
      601 tctccggcca ttcctgtttc tacccaggag tccgtttctg ttccagcaag tgtgagcacc
      661 agcattagca gtattagcac cggtgccacc gctagcgccc tggttaaagg caataccaat
      721 ccgattacaa gcatgtctgc c
//
