LOCUS       sequence-271118-        4089 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing SGK1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_SGK1_WT
FEATURES             Location/Qualifiers
     source          1..4089
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1968..2067)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2084..2103)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2085..2103)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2108..2124)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2108..2124)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2555..3346
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3439..4027
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3928..3947
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atgacggtga aaactgaggc tgctaagggc accctcactt actccaggat
      721 gaggggcatg gtggcaattc tcatcgcttt catgaagcag aggaggatgg gtctgaacga
      781 ctttattcag aagattgcca ataactccta tgcatgcaaa caccctgaag ttcagtccat
      841 cttgaagatc tcccaacctc aggagcctga gcttatgaat gccaaccctt ctcctccacc
      901 aagtccttct cagcaaatca accttggccc gtcgtccaat cctcatgcta aaccatctga
      961 ctttcacttc ttgaaagtga tcggaaaggg cagttttgga aaggttcttc tagcaagaca
     1021 caaggcagaa gaagtgttct atgcagtcaa agttttacag aagaaagcaa tcctgaaaaa
     1081 gaaagaggag aagcatatta tgtcggagcg gaatgttctg ttgaagaatg tgaagcaccc
     1141 tttcctggtg ggccttcact tctctttcca gactgctgac aaattgtact ttgtcctaga
     1201 ctacattaat ggtggagagt tgttctacca tctccagagg gaacgctgct tcctggaacc
     1261 acgggctcgt ttctatgctg ctgaaatagc cagtgccttg ggctacctgc attcactgaa
     1321 catcgtttat agagacttaa aaccagagaa tattttgcta gattcacagg gacacattgt
     1381 ccttactgac ttcggactct gcaaggagaa cattgaacac aacagcacaa catccacctt
     1441 ctgtggcacg ccggagtatc tcgcacctga ggtgcttcat aagcagcctt atgacaggac
     1501 tgtggactgg tggtgcctgg gagctgtctt gtatgagatg ctgtatggcc tgccgccttt
     1561 ttatagccga aacacagctg aaatgtacga caacattctg aacaagcctc tccagctgaa
     1621 accaaatatt acaaattccg caagacacct cctggagggc ctcctgcaga aggacaggac
     1681 aaagcggctc ggggccaagg atgacttcat ggagattaag agtcatgtct tcttctcctt
     1741 aattaactgg gatgatctca ttaataagaa gattactccc ccttttaacc caaatgtgag
     1801 tgggcccaac gacctacggc actttgaccc cgagtttacc gaagagcctg tccccaactc
     1861 cattggcaag tcccctgaca gcgtcctcgt cacagccagc gtcaaggaag ctgccgaggc
     1921 tttcctaggc ttttcctatg cgcctcccac ggactctttc ctctgattgc caactttctt
     1981 gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac
     2041 tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta
     2101 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
     2161 cattgcacaa gataaaaata tatcatcatg cctcctctag accagccagg acagaaatgc
     2221 ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc
     2281 acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg
     2341 ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt
     2401 tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca
     2461 gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca
     2521 gcagggcagt cgccctaaaa caaagttaaa cattatgagg gaagcggtga tcgccgaagt
     2581 atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct
     2641 ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga
     2701 tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga
     2761 ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac
     2821 cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt
     2881 tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat
     2941 tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc
     3001 ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac
     3061 cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac
     3121 gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc
     3181 cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca
     3241 ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt
     3301 tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct cgagccaccc
     3361 atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga
     3421 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     3481 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     3541 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     3601 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     3661 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     3721 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     3781 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     3841 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     3901 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     3961 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     4021 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     4081 tcacatgtt
//
