LOCUS       sequence-271149-       10285 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of myc-tagged HLA-A*02:01.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10285)
  AUTHORS   McShan AC, Devlin CA, Overall SA, Park J, Toor JS, Moschidi D, 
            Flores-Solis D, Choi H, Tripathi S, Procko E, Sgourakis NG
  TITLE     Molecular determinants of chaperone interactions on MHC-I for 
            folding and antigen repertoire selection.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25602-25613. doi: 
            10.1073/pnas.1915562116. Epub 2019 Dec 3.
  PUBMED    31796585
REFERENCE   2  (bases 1 to 10285)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1915562116"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2019-12-17- 17"; volume: "116"; issue: "51"; pages: 
            "25602-25613"
COMMENT     Sequence Label: pCEP4-myc-HLA-A2
FEATURES             Location/Qualifiers
     source          1..10285
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(370..388)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        456..560
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(561..629,630..1421)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(779..798)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1592..2180
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2081..2100
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2611..2756
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             2799..3836
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(3834..3853)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3878..3926
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4580..4959
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4960..5163
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5114..5134
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5160..5184
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5266..5295
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    6371..6505
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6395..6414)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6449..6468
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(6648..6670)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7230..9019
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 agactcctgc cccctccaac agccccccca ccctcatccc cttcatggtc gctgtcagac
       61 agatccaggt ctgaaaattc cccatcctcc gaaccatcct cgtcctcatc accaattact
      121 cgcagcccgg aaaactcccg ctgaacatcc tcaagatttg cgtcctgagc ctcaagccag
      181 gcctcaaatt cctcgtcccc ctttttgctg gacggtaggg atggggattc tcgggacccc
      241 tcctcttcct cttcaaggtc accagacaga gatgctactg gggcaacgga agaaaagctg
      301 ggtgcggcct gtgaggatca gcttatcgat gataagctgt caaacatgag aattcttgaa
      361 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
      421 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      481 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      541 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      601 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      661 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      721 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      781 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac
      841 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      901 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      961 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     1021 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     1081 acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg
     1141 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     1201 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     1261 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     1321 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1381 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1441 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1501 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1561 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1621 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1681 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1741 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1801 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1861 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1921 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1981 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     2041 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     2101 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     2161 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     2221 gctggccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca
     2281 acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc
     2341 ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccccgaga tgcgccgcgt
     2401 gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt
     2461 cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag
     2521 gtgccgccct gcttcatccc cgtggcccgt tgctcgcgtt tgctggcggt gtccccggaa
     2581 gaaatatatt tgcatgtctt tagttctatg atgacacaaa ccccgcccag cgtcttgtca
     2641 ttggcgaatt cgaacacgca gatgcagtcg gggcggcgcg gtccgaggtc cacttcgcat
     2701 attaaggtga cgcgtgtggc ctcgaacacc gagcgaccct gcagcgaccc gcttaacagc
     2761 gtcaacagcg tgccgcagat cccggggggc aatgagatat gaaaaagcct gaactcaccg
     2821 cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc
     2881 tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc
     2941 tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg
     3001 catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaattcagc gagagcctga
     3061 cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac
     3121 tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta
     3181 gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc
     3241 gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg
     3301 acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact
     3361 gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca
     3421 atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg
     3481 aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct
     3541 acttcgagcg gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc
     3601 gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt
     3661 gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac
     3721 aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata
     3781 gtggaaaccg acgccccagc actcgtccgg atcgggagat gggggaggct aactgaaaca
     3841 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa
     3901 acgcacgggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc
     3961 tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc
     4021 accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg
     4081 ccctgccata gccactggcc ccgtgggtta gggacggggt cccccatggg gaatggttta
     4141 tggttcgtgg gggttattat tttgggcgtt gcgtggggtc aggtccacga ctggactgag
     4201 cagacagacc catggttttt ggatggcctg ggcatggacc gcatgtactg gcgcgacacg
     4261 aacaccgggc gtctgtggct gccaaacacc cccgaccccc aaaaaccacc gcgcggattt
     4321 ctggcgtgcc aagctagtcg accaattctc atgtttgaca gcttatcatc gcagatccgg
     4381 gcaacgttgt tgccattgct gcaggcgcag aactggtagg tatggaagat ctatacattg
     4441 aatcaatatt ggcaattagc catattagtc attggttata tagcataaat caatattggc
     4501 tattggccat tgcatacgtt gtatctatat cataatatgt acatttatat tggctcatgt
     4561 ccaatatgac cgccatgttg acattgatta ttgactagtt attaatagta atcaattacg
     4621 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     4681 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     4741 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     4801 gcccacttgg cagtacatca agtgtatcat atgccaagtc cgccccctat tgacgtcaat
     4861 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttacggga ctttcctact
     4921 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     4981 accaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     5041 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaataac
     5101 cccgccccgt tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     5161 gctcgtttag tgaaccgtca gatctctaga agctgggtac cagctgctag cgccaccatg
     5221 aagaccatca tcgccctgag ctacatcttc tgcctggtgt tcgccgagca gaaattgatc
     5281 agcgaggagg acctgggcag ccctggaggc agttcaggag gcggatctca ctccatgagg
     5341 tatttcttca catccgtgtc ccggcccggc cgcggggagc cccgcttcat cgcagtgggc
     5401 tacgtggacg acacgcagtt cgtgcggttc gacagcgacg ccgcgagcca gaggatggag
     5461 ccgcgggcgc cgtggataga gcaggagggt ccggagtatt gggacgggga gacacggaaa
     5521 gtgaaggccc actcacagac tcaccgagtg gacctgggga ccctgcgcgg ctactacaac
     5581 cagagcgagg ccggttctca caccgtccag aggatgtatg gctgcgacgt ggggtcggac
     5641 tggcgcttcc tccgcgggta ccaccagtac gcctacgacg gcaaggatta catcgccctg
     5701 aaagaggacc tgcgctcttg gaccgcggcg gacatggcag ctcagaccac caagcacaag
     5761 tgggaggcgg cccatgtggc ggagcagttg agagcctacc tggagggcac gtgcgtggag
     5821 tggctccgca gatacctgga gaacgggaag gagacgctgc agcgtacgga cgcccccaaa
     5881 acgcatatga ctcaccacgc tgtctctgac catgaagcca ccctgaggtg ctgggccctg
     5941 agcttctacc ctgcggagat cacactgacc tggcagcggg atggggagga ccagacccag
     6001 gacacggagc tcgtggagac caggcctgca ggggatggaa ccttccagaa gtgggcggct
     6061 gtggtggtgc cttctggaca ggagcagaga tacacctgcc atgtgcagca tgagggtttg
     6121 cccaagcccc tcaccctgag atgggagccg tcttcccagc ccaccatccc catcgtgggc
     6181 atcattgctg gcctggttct ctttggagct gtgatcactg gagctgtggt cgctgctgtg
     6241 atgtggagga ggaagagctc agatagaaaa ggagggagct actctcaggc tgcaagcagt
     6301 gacagtgccc agggctctga tgtgtctctc acagcttgta aagtgtgata actcgaggcc
     6361 ggcaaggccg gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
     6421 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     6481 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     6541 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc
     6601 tgattatgat ccggctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     6661 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     6721 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaggtc gactctagag
     6781 gatcgatccc cgccccggac gaactaaacc tgactacgac atctctgccc cttcttcgcg
     6841 gggcagtgca tgtaatccct tcagttggtt ggtacaactt gccaactggg ccctgttcca
     6901 catgtgacac ggggggggac caaacacaaa ggggttctct gactgtagtt gacatcctta
     6961 taaatggatg tgcacatttg ccaacactga gtggctttca tcctggagca gactttgcag
     7021 tctgtggact gcaacacaac attgccttta tgtgtaactc ttggctgaag ctcttacacc
     7081 aatgctgggg gacatgtacc tcccaggggc ccaggaagac tacgggaggc tacaccaacg
     7141 tcaatcagag gggcctgtgt agctaccgat aagcggaccc tcaagagggc attagcaata
     7201 gtgtttataa ggcccccttg ttaaccctaa acgggtagca tatgcttccc gggtagtagt
     7261 atatactatc cagactaacc ctaattcaat agcatatgtt acccaacggg aagcatatgc
     7321 tatcgaatta gggttagtaa aagggtccta aggaacagcg atatctccca ccccatgagc
     7381 tgtcacggtt ttatttacat ggggtcagga ttccacgagg gtagtgaacc attttagtca
     7441 caagggcagt ggctgaagat caaggagcgg gcagtgaact ctcctgaatc ttcgcctgct
     7501 tcttcattct ccttcgttta gctaatagaa taactgctga gttgtgaaca gtaaggtgta
     7561 tgtgaggtgc tcgaaaacaa ggtttcaggt gacgccccca gaataaaatt tggacggggg
     7621 gttcagtggt ggcattgtgc tatgacacca atataaccct cacaaacccc ttgggcaata
     7681 aatactagtg taggaatgaa acattctgaa tatctttaac aatagaaatc catggggtgg
     7741 ggacaagccg taaagactgg atgtccatct cacacgaatt tatggctatg ggcaacacat
     7801 aatcctagtg caatatgata ctggggttat taagatgtgt cccaggcagg gaccaagaca
     7861 ggtgaaccat gttgttacac tctatttgta acaaggggaa agagagtgga cgccgacagc
     7921 agcggactcc actggttgtc tctaacaccc ccgaaaatta aacggggctc cacgccaatg
     7981 gggcccataa acaaagacaa gtggccactc ttttttttga aattgtggag tgggggcacg
     8041 cgtcagcccc cacacgccgc cctgcggttt tggactgtaa aataagggtg taataacttg
     8101 gctgattgta accccgctaa ccactgcggt caaaccactt gcccacaaaa ccactaatgg
     8161 caccccgggg aatacctgca taagtaggtg ggcgggccaa gataggggcg cgattgctgc
     8221 gatctggagg acaaattaca cacacttgcg cctgagcgcc aagcacaggg ttgttggtcc
     8281 tcatattcac gaggtcgctg agagcacggt gggctaatgt tgccatgggt agcatatact
     8341 acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca taggctatcc
     8401 taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc
     8461 taatttatat ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc
     8521 taatctatat ctgggtagta tatgctatcc taatctgtat ccgggtagca tatgctatcc
     8581 taatagagat tagggtagta tatgctatcc taatttatat ctgggtagca tatactaccc
     8641 aaatatctgg atagcatatg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     8701 ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     8761 ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat
     8821 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     8881 ctgtatccgg gtagcatatg ctatcctcat gcatatacag tcagcatatg atacccagta
     8941 gtagagtggg agtgctatcc tttgcatatg ccgccacctc ccaagggggc gtgaattttc
     9001 gctgcttgtc cttttcctgc tggttgctcc cattcttagg tgaatttaag gaggccaggc
     9061 taaagccgtc gcatgtctga ttgctcacca ggtaaatgtc gctaatgttt tccaacgcga
     9121 gaaggtgttg agcgcggagc tgagtgacgt gacaacatgg gtatgcccaa ttgccccatg
     9181 ttgggaggac gaaaatggtg acaagacaga tggccagaaa tacaccaaca gcacgcatga
     9241 tgtctactgg ggatttattc tttagtgcgg gggaatacac ggcttttaat acgattgagg
     9301 gcgtctccta acaagttaca tcactcctgc ccttcctcac cctcatctcc atcacctcct
     9361 tcatctccgt catctccgtc atcaccctcc gcggcagccc cttccaccat aggtggaaac
     9421 cagggaggca aatctactcc atcgtcaaag ctgcacacag tcaccctgat attgcaggta
     9481 ggagcgggct ttgtcataac aaggtcctta atcgcatcct tcaaaacctc agcaaatata
     9541 tgagtttgta aaaagaccat gaaataacag acaatggact cccttagcgg gccaggttgt
     9601 gggccgggtc caggggccat tccaaagggg agacgactca atggtgtaag acgacattgt
     9661 ggaatagcaa gggcagttcc tcgccttagg ttgtaaaggg aggtcttact acctccatat
     9721 acgaacacac cggcgaccca agttccttcg tcggtagtcc tttctacgtg actcctagcc
     9781 aggagagctc ttaaaccttc tgcaatgttc tcaaatttcg ggttggaacc tccttgacca
     9841 cgatgctttc caaaccaccc tccttttttg cgcctgcctc catcaccctg accccggggt
     9901 ccagtgcttg ggccttctcc tgggtcatct gcggggccct gctctatcgc tcccgggggc
     9961 acgtcaggct caccatctgg gccaccttct tggtggtatt caaaataatc ggcttcccct
    10021 acagggtgga aaaatggcct tctacctgga gggggcctgc gcggtggaga cccggatgat
    10081 gatgactgac tactgggact cctgggcctc ttttctccac gtccacgacc tctccccctg
    10141 gctctttcac gacttccccc cctggctctt tcacgtcctc taccccggcg gcctccacta
    10201 cctcctcgac cccggcctcc actacctcct cgaccccggc ctccactgcc tcctcgaccc
    10261 cggcctccac ctcctgctcc tgccc
//
