LOCUS       sequence-27115-e         610 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Jho EH, Zhang T, Domon C, Joo CK, Freund JN, Costantini F
  TITLE     Wnt/beta-catenin/Tcf signaling induces the transcription of Axin2, a
            negative regulator of the signaling pathway.
  JOURNAL   Mol Cell Biol. 2002 Feb . 22(4):1172-83.
  PUBMED    11809808
REFERENCE   2  (bases 1 to 610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2002 Feb . 22(4):1172-83."
COMMENT     Sequence Label: Axin2 full length cDNA
FEATURES             Location/Qualifiers
     source          1..610
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(39..57)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(40..57)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(76..92)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(76..92)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(89..111)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    100..116
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(124..130,131..148,149..154))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    169..190
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(307..324)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(558..577)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctctagagt cgacctgcag gcatgcaagc ttgagtattc tatagtgtca cctaaatacn
       61 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
      121 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
      181 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
      241 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
      301 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
      361 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
      421 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
      481 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
      541 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
      601 tcctgtcacc
//
