LOCUS       sequence-271150-        7729 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of myc-tagged HLA-B*15:01.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7729)
  AUTHORS   McShan AC, Devlin CA, Overall SA, Park J, Toor JS, Moschidi D, 
            Flores-Solis D, Choi H, Tripathi S, Procko E, Sgourakis NG
  TITLE     Molecular determinants of chaperone interactions on MHC-I for 
            folding and antigen repertoire selection.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25602-25613. doi: 
            10.1073/pnas.1915562116. Epub 2019 Dec 3.
  PUBMED    31796585
REFERENCE   2  (bases 1 to 7729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1915562116"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2019-12-17- 17"; volume: "116"; issue: "51"; pages: 
            "25602-25613"
COMMENT     Sequence Label: pCEP4-myc-HLA-B15
FEATURES             Location/Qualifiers
     source          1..7729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(720..738)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        806..910
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(911..979,980..1771)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1129..1148)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1942..2530
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2431..2450
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2961..3106
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3149..4186
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4184..4203)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4228..4276
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4930..5309
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5310..5513
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5464..5484
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5510..5534
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5616..5645
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    6712..6846
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6736..6755)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6790..6809
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(6989..7011)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 cgctcctgct cctgctcctg ttccaccgtg ggtccctttg cagccaatgc aacttggacg
       61 tttttggggt ctccggacac catctctatg tcttggccct gatcctgagc cgcccggggc
      121 tcctggtctt ccgcctcctc gtcctcgtcc tcttccccgt cctcgtccat ggttatcacc
      181 ccctcttctt tgaggtccac tgccgccgga gccttctggt ccagatgtgt ctcccttctc
      241 tcctaggcca tttccaggtc ctgtacctgg cccctcgtca gacatgattc acactaaaag
      301 agatcaatag acatctttat tagacgacgc tcagtgaata cagggagtgc agactcctgc
      361 cccctccaac agccccccca ccctcatccc cttcatggtc gctgtcagac agatccaggt
      421 ctgaaaattc cccatcctcc gaaccatcct cgtcctcatc accaattact cgcagcccgg
      481 aaaactcccg ctgaacatcc tcaagatttg cgtcctgagc ctcaagccag gcctcaaatt
      541 cctcgtcccc ctttttgctg gacggtaggg atggggattc tcgggacccc tcctcttcct
      601 cttcaaggtc accagacaga gatgctactg gggcaacgga agaaaagctg ggtgcggcct
      661 gtgaggatca gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg
      721 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
      781 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
      841 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      901 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      961 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     1021 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     1081 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     1141 ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     1201 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     1261 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     1321 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     1381 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     1441 caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     1501 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     1561 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     1621 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     1681 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     1741 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1801 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1861 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1921 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1981 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     2041 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     2101 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     2161 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     2221 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     2281 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     2341 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     2401 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     2461 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     2521 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttg
     2581 aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat
     2641 cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc
     2701 gaacgccagc aagacgtagc ccagcgcgtc ggccccgaga tgcgccgcgt gcggctgctg
     2761 gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc
     2821 cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccct
     2881 gcttcatccc cgtggcccgt tgctcgcgtt tgctggcggt gtccccggaa gaaatatatt
     2941 tgcatgtctt tagttctatg atgacacaaa ccccgcccag cgtcttgtca ttggcgaatt
     3001 cgaacacgca gatgcagtcg gggcggcgcg gtccgaggtc cacttcgcat attaaggtga
     3061 cgcgtgtggc ctcgaacacc gagcgaccct gcagcgaccc gcttaacagc gtcaacagcg
     3121 tgccgcagat cccggggggc aatgagatat gaaaaagcct gaactcaccg cgacgtctgt
     3181 cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg
     3241 cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa
     3301 tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc
     3361 gctcccgatt ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat
     3421 ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt
     3481 tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag
     3541 cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat
     3601 atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag
     3661 tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt
     3721 ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat
     3781 aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa
     3841 catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg
     3901 gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct
     3961 tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg
     4021 tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg
     4081 cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg
     4141 acgccccagc actcgtccgg atcgggagat gggggaggct aactgaaaca cggaaggaga
     4201 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacgggt
     4261 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc
     4321 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc
     4381 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata
     4441 gccactggcc ccgtgggtta gggacggggt cccccatggg gaatggttta tggttcgtgg
     4501 gggttattat tttgggcgtt gcgtggggtc aggtccacga ctggactgag cagacagacc
     4561 catggttttt ggatggcctg ggcatggacc gcatgtactg gcgcgacacg aacaccgggc
     4621 gtctgtggct gccaaacacc cccgaccccc aaaaaccacc gcgcggattt ctggcgtgcc
     4681 aagctagtcg accaattctc atgtttgaca gcttatcatc gcagatccgg gcaacgttgt
     4741 tgccattgct gcaggcgcag aactggtagg tatggaagat ctatacattg aatcaatatt
     4801 ggcaattagc catattagtc attggttata tagcataaat caatattggc tattggccat
     4861 tgcatacgtt gtatctatat cataatatgt acatttatat tggctcatgt ccaatatgac
     4921 cgccatgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
     4981 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     5041 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     5101 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     5161 cagtacatca agtgtatcat atgccaagtc cgccccctat tgacgtcaat gacggtaaat
     5221 ggcccgcctg gcattatgcc cagtacatga ccttacggga ctttcctact tggcagtaca
     5281 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac accaatgggc
     5341 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     5401 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaataac cccgccccgt
     5461 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag
     5521 tgaaccgtca gatctctaga agctgggtac cagctgctag cgccaccatg aagaccatca
     5581 tcgccctgag ctacatcttc tgcctggtgt tcgccgagca gaaattgatc agcgaggagg
     5641 acctgggcag ccctggaggc agttcaggag gcggatctca ttctatgaga tatttctaca
     5701 ccgccatgtc ccggcccggc cgcggggagc cccgcttcat cgcagtgggc tacgtggacg
     5761 acacccagtt cgtgaggttc gacagcgacg ccgcgagtcc gaggatggcg ccccgggcgc
     5821 catggataga gcaggagggg ccggagtatt gggaccggga gacacagatc tccaagacca
     5881 acacacagac ttaccgagag agcctgcgga acctgcgcgg ctactacaac cagagcgagg
     5941 ccgggtctca caccctccag aggatgtacg gctgcgacgt ggggccggac gggcgcctcc
     6001 tccgcgggca tgaccagtcc gcctacgacg gcaaggatta catcgccctg aacgaggacc
     6061 tgagctcctg gaccgcggcg gacacggcgg ctcagatcac ccagcgcaag tgggaggcgg
     6121 cccgtgaggc ggagcagtgg agagcctacc tggagggcct gtgcgtggag tggctccgca
     6181 gatacctgga gaacgggaag gagacgctgc agcgcgcgga ccccccaaag acacatgtga
     6241 cccaccaccc catctctgac catgaggcca ccctgaggtg ctgggccctg ggcttctacc
     6301 ctgcggagat cacactgacc tggcagcggg atggcgagga ccaaactcag gacaccgagc
     6361 ttgtggagac cagaccagca ggagatagaa ccttccagaa gtgggcagct gtggtggtgc
     6421 cttctggaga agagcagaga tacacatgcc atgtacagca tgaggggctg ccgaagcccc
     6481 tcaccctgag atgggagcca tcttcccagt ccacgatacc catcgtaggg atcgttgcgg
     6541 gactcgccgt cttggctgtg gtagtgatcg gcgcggtcgt tgcaactgta atgtgtcggc
     6601 gaaaatcatc tggtggaaag ggcggttcat attcccaagc tgcatcctct gattcagccc
     6661 aggggtccga tgtgtcattg acagcatgat aactcgaggc cggcaaggcc ggatccagac
     6721 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     6781 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     6841 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
     6901 gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tccggctgcc
     6961 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     7021 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     7081 ttggcgggtg tcggggcgca gccatgaggt cgactctaga ggatcgatcc ccgccccgga
     7141 cgaactaaac ctgactacga catctctgcc ccttcttcgc ggggcagtgc atgtaatccc
     7201 ttcagttggt tggtacaact tgccaactgg gccctgttcc acatgtgaca cgggggggga
     7261 ccaaacacaa aggggttctc tgactgtagt tgacatcctt ataaatggat gtgcacattt
     7321 gccaacactg agtggctttc atcctggagc agactttgca gtctgtggac tgcaacacaa
     7381 cattgccttt atgtgtaact cttggctgaa gctcttacac caatgctggg ggacatgtac
     7441 ctcccagggg cccaggaaga ctacgggagg ctacaccaac gtcaatcaga ggggcctgtg
     7501 tagctaccga taagcggacc ctcaagaggg cattagcaat agtgtttata aggccccctt
     7561 gttaacccta aacgggtagc atatgcttcc cgggtagtag tatatactat ccagactaac
     7621 cctaattcaa tagcatatgt tacccaacgg gaagcatatg ctatcgaatt agggttagta
     7681 aaagggtcct aaggaacagc gatatctccc accccatgag ctgtcacgg
//
