LOCUS       sequence-271151-        9955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of myc-tagged HLA-B*08:01.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9955)
  AUTHORS   McShan AC, Devlin CA, Overall SA, Park J, Toor JS, Moschidi D, 
            Flores-Solis D, Choi H, Tripathi S, Procko E, Sgourakis NG
  TITLE     Molecular determinants of chaperone interactions on MHC-I for 
            folding and antigen repertoire selection.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25602-25613. doi: 
            10.1073/pnas.1915562116. Epub 2019 Dec 3.
  PUBMED    31796585
REFERENCE   2  (bases 1 to 9955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1915562116"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2019-12-17- 17"; volume: "116"; issue: "51"; pages: 
            "25602-25613"
COMMENT     Sequence Label: pCEP4-myc-HLA-B8
FEATURES             Location/Qualifiers
     source          1..9955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(66..84)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        152..256
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(257..325,326..1117)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(475..494)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1288..1876
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1777..1796
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2307..2452
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             2495..3532
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(3530..3549)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3574..3622
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4276..4655
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4656..4859
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4810..4830
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4856..4880
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4962..4991
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    6058..6192
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6082..6101)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6136..6155
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(6335..6357)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6917..8706
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 cggcctgtga ggatcagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg
       61 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
      121 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
      181 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
      241 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
      301 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
      361 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
      421 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
      481 tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
      541 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
      601 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
      661 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
      721 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
      781 gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga
      841 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
      901 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
      961 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     1021 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     1081 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     1141 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     1201 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1261 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1321 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1381 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     1441 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1501 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1561 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1621 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     1681 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1741 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1801 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1861 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     1921 gccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc
     1981 gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg
     2041 cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc ccgagatgcg ccgcgtgcgg
     2101 ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca
     2161 gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc
     2221 cgccctgctt catccccgtg gcccgttgct cgcgtttgct ggcggtgtcc ccggaagaaa
     2281 tatatttgca tgtctttagt tctatgatga cacaaacccc gcccagcgtc ttgtcattgg
     2341 cgaattcgaa cacgcagatg cagtcggggc ggcgcggtcc gaggtccact tcgcatatta
     2401 aggtgacgcg tgtggcctcg aacaccgagc gaccctgcag cgacccgctt aacagcgtca
     2461 acagcgtgcc gcagatcccg gggggcaatg agatatgaaa aagcctgaac tcaccgcgac
     2521 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc
     2581 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg
     2641 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc
     2701 ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta
     2761 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc
     2821 cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca
     2881 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga
     2941 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac
     3001 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc
     3061 cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg
     3121 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt
     3181 cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt
     3241 cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat
     3301 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc
     3361 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat
     3421 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg
     3481 aaaccgacgc cccagcactc gtccggatcg ggagatgggg gaggctaact gaaacacgga
     3541 aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc
     3601 acgggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc
     3661 gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc
     3721 caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct
     3781 gccatagcca ctggccccgt gggttaggga cggggtcccc catggggaat ggtttatggt
     3841 tcgtgggggt tattattttg ggcgttgcgt ggggtcaggt ccacgactgg actgagcaga
     3901 cagacccatg gtttttggat ggcctgggca tggaccgcat gtactggcgc gacacgaaca
     3961 ccgggcgtct gtggctgcca aacacccccg acccccaaaa accaccgcgc ggatttctgg
     4021 cgtgccaagc tagtcgacca attctcatgt ttgacagctt atcatcgcag atccgggcaa
     4081 cgttgttgcc attgctgcag gcgcagaact ggtaggtatg gaagatctat acattgaatc
     4141 aatattggca attagccata ttagtcattg gttatatagc ataaatcaat attggctatt
     4201 ggccattgca tacgttgtat ctatatcata atatgtacat ttatattggc tcatgtccaa
     4261 tatgaccgcc atgttgacat tgattattga ctagttatta atagtaatca attacggggt
     4321 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     4381 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     4441 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     4501 acttggcagt acatcaagtg tatcatatgc caagtccgcc ccctattgac gtcaatgacg
     4561 gtaaatggcc cgcctggcat tatgcccagt acatgacctt acgggacttt cctacttggc
     4621 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacacca
     4681 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
     4741 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aataaccccg
     4801 ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
     4861 gtttagtgaa ccgtcagatc tctagaagct gggtaccagc tgctagcgcc accatgaaga
     4921 ccatcatcgc cctgagctac atcttctgcc tggtgttcgc cgagcagaaa ttgatcagcg
     4981 aggaggacct gggcagccct ggaggcagtt caggaggcgg atctcattct atgagatatt
     5041 tcgacaccgc catgtcccgg cccggccgcg gggagccccg cttcatctca gtgggctacg
     5101 tggacgacac gcagttcgtg aggttcgaca gcgacgccgc gagtccgaga gaggagccgc
     5161 gggcgccgtg gatagagcag gaggggccgg agtattggga ccggaacaca cagatcttca
     5221 agaccaacac acagactgac cgagagagcc tgcggaacct gcgcggctac tacaaccaga
     5281 gcgaggccgg gtctcacacc ctccagagca tgtacggctg cgacgtgggg ccggacgggc
     5341 gcctcctccg cgggcataac cagtacgcct acgacggcaa ggattacatc gccctgaacg
     5401 aggacctgcg ctcctggacc gcggcggaca ccgcggctca gatcacccag cgcaagtggg
     5461 aggcggcccg tgtggcggag caggacagag cctacctgga gggcacgtgc gtggagtggc
     5521 tccgcagata cctggagaac gggaaggaca cgctggagcg cgcggacccc ccaaagacac
     5581 acgtgaccca ccaccccatc tctgaccatg aggccaccct gaggtgctgg gccctgggct
     5641 tctaccctgc ggagatcaca ctgacctggc agcgggatgg cgaggaccaa actcaggaca
     5701 ctgagcttgt ggagaccaga ccagcaggag atagaacctt ccagaagtgg gcagctgtgg
     5761 tggtgccttc tggagaagag cagagataca catgccatgt acagcatgag gggctgccta
     5821 agcccctcac cctgagatgg gagccgtctt cccagtccac tgttcccatc gtcggtatag
     5881 ttgctggcct cgcagtcttg gcggtagtag tcattggagc tgtagtggca gcagtgatgt
     5941 gcagacgcaa gagttccggc ggcaagggag gctcctattc tcaagctgct tgctccgact
     6001 cagcacaagg cagtgatgtc tcattgactg catgataact cgaggccggc aaggccggat
     6061 ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     6121 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     6181 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
     6241 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatccg
     6301 gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     6361 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     6421 cgggtgttgg cgggtgtcgg ggcgcagcca tgaggtcgac tctagaggat cgatccccgc
     6481 cccggacgaa ctaaacctga ctacgacatc tctgcccctt cttcgcgggg cagtgcatgt
     6541 aatcccttca gttggttggt acaacttgcc aactgggccc tgttccacat gtgacacggg
     6601 gggggaccaa acacaaaggg gttctctgac tgtagttgac atccttataa atggatgtgc
     6661 acatttgcca acactgagtg gctttcatcc tggagcagac tttgcagtct gtggactgca
     6721 acacaacatt gcctttatgt gtaactcttg gctgaagctc ttacaccaat gctgggggac
     6781 atgtacctcc caggggccca ggaagactac gggaggctac accaacgtca atcagagggg
     6841 cctgtgtagc taccgataag cggaccctca agagggcatt agcaatagtg tttataaggc
     6901 ccccttgtta accctaaacg ggtagcatat gcttcccggg tagtagtata tactatccag
     6961 actaacccta attcaatagc atatgttacc caacgggaag catatgctat cgaattaggg
     7021 ttagtaaaag ggtcctaagg aacagcgata tctcccaccc catgagctgt cacggtttta
     7081 tttacatggg gtcaggattc cacgagggta gtgaaccatt ttagtcacaa gggcagtggc
     7141 tgaagatcaa ggagcgggca gtgaactctc ctgaatcttc gcctgcttct tcattctcct
     7201 tcgtttagct aatagaataa ctgctgagtt gtgaacagta aggtgtatgt gaggtgctcg
     7261 aaaacaaggt ttcaggtgac gcccccagaa taaaatttgg acggggggtt cagtggtggc
     7321 attgtgctat gacaccaata taaccctcac aaaccccttg ggcaataaat actagtgtag
     7381 gaatgaaaca ttctgaatat ctttaacaat agaaatccat ggggtgggga caagccgtaa
     7441 agactggatg tccatctcac acgaatttat ggctatgggc aacacataat cctagtgcaa
     7501 tatgatactg gggttattaa gatgtgtccc aggcagggac caagacaggt gaaccatgtt
     7561 gttacactct atttgtaaca aggggaaaga gagtggacgc cgacagcagc ggactccact
     7621 ggttgtctct aacacccccg aaaattaaac ggggctccac gccaatgggg cccataaaca
     7681 aagacaagtg gccactcttt tttttgaaat tgtggagtgg gggcacgcgt cagcccccac
     7741 acgccgccct gcggttttgg actgtaaaat aagggtgtaa taacttggct gattgtaacc
     7801 ccgctaacca ctgcggtcaa accacttgcc cacaaaacca ctaatggcac cccggggaat
     7861 acctgcataa gtaggtgggc gggccaagat aggggcgcga ttgctgcgat ctggaggaca
     7921 aattacacac acttgcgcct gagcgccaag cacagggttg ttggtcctca tattcacgag
     7981 gtcgctgaga gcacggtggg ctaatgttgc catgggtagc atatactacc caaatatctg
     8041 gatagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg
     8101 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg
     8161 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     8221 ggtagtatat gctatcctaa tctgtatccg ggtagcatat gctatcctaa tagagattag
     8281 ggtagtatat gctatcctaa tttatatctg ggtagcatat actacccaaa tatctggata
     8341 gcatatgcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     8401 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     8461 gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta
     8521 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta
     8581 gcatatgcta tcctcatgca tatacagtca gcatatgata cccagtagta gagtgggagt
     8641 gctatccttt gcatatgccg ccacctccca agggggcgtg aattttcgct gcttgtcctt
     8701 ttcctgctgg ttgctcccat tcttaggtga atttaaggag gccaggctaa agccgtcgca
     8761 tgtctgattg ctcaccaggt aaatgtcgct aatgttttcc aacgcgagaa ggtgttgagc
     8821 gcggagctga gtgacgtgac aacatgggta tgcccaattg ccccatgttg ggaggacgaa
     8881 aatggtgaca agacagatgg ccagaaatac accaacagca cgcatgatgt ctactgggga
     8941 tttattcttt agtgcggggg aatacacggc ttttaatacg attgagggcg tctcctaaca
     9001 agttacatca ctcctgccct tcctcaccct catctccatc acctccttca tctccgtcat
     9061 ctccgtcatc accctccgcg gcagcccctt ccaccatagg tggaaaccag ggaggcaaat
     9121 ctactccatc gtcaaagctg cacacagtca ccctgatatt gcaggtagga gcgggctttg
     9181 tcataacaag gtccttaatc gcatccttca aaacctcagc aaatatatga gtttgtaaaa
     9241 agaccatgaa ataacagaca atggactccc ttagcgggcc aggttgtggg ccgggtccag
     9301 gggccattcc aaaggggaga cgactcaatg gtgtaagacg acattgtgga atagcaaggg
     9361 cagttcctcg ccttaggttg taaagggagg tcttactacc tccatatacg aacacaccgg
     9421 cgacccaagt tccttcgtcg gtagtccttt ctacgtgact cctagccagg agagctctta
     9481 aaccttctgc aatgttctca aatttcgggt tggaacctcc ttgaccacga tgctttccaa
     9541 accaccctcc ttttttgcgc ctgcctccat caccctgacc ccggggtcca gtgcttgggc
     9601 cttctcctgg gtcatctgcg gggccctgct ctatcgctcc cgggggcacg tcaggctcac
     9661 catctgggcc accttcttgg tggtattcaa aataatcggc ttcccctaca gggtggaaaa
     9721 atggccttct acctggaggg ggcctgcgcg gtggagaccc ggatgatgat gactgactac
     9781 tgggactcct gggcctcttt tctccacgtc cacgacctct ccccctggct ctttcacgac
     9841 ttccccccct ggctctttca cgtcctctac cccggcggcc tccactacct cctcgacccc
     9901 ggcctccact acctcctcga ccccggcctc cactgcctcc tcgaccccgg cctcc
//
