LOCUS       sequence-271152-        7384 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of myc-tagged H2-Ld.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7384)
  AUTHORS   McShan AC, Devlin CA, Overall SA, Park J, Toor JS, Moschidi D, 
            Flores-Solis D, Choi H, Tripathi S, Procko E, Sgourakis NG
  TITLE     Molecular determinants of chaperone interactions on MHC-I for 
            folding and antigen repertoire selection.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25602-25613. doi: 
            10.1073/pnas.1915562116. Epub 2019 Dec 3.
  PUBMED    31796585
REFERENCE   2  (bases 1 to 7384)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1915562116"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2019-12-17- 17"; volume: "116"; issue: "51"; pages: 
            "25602-25613"
COMMENT     Sequence Label: pCEP4-myc-H2-Ld
FEATURES             Location/Qualifiers
     source          1..7384
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(253..271)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        339..443
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(444..512,513..1304)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(662..681)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1475..2063
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1964..1983
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2494..2639
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             2682..3719
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(3717..3736)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3761..3809
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4463..4842
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4843..5046
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4997..5017
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5043..5067
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5149..5178
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    6245..6379
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6269..6288)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6323..6342
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(6522..6544)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 actcgcagcc cggaaaactc ccgctgaaca tcctcaagat ttgcgtcctg agcctcaagc
       61 caggcctcaa attcctcgtc cccctttttg ctggacggta gggatgggga ttctcgggac
      121 ccctcctctt cctcttcaag gtcaccagac agagatgcta ctggggcaac ggaagaaaag
      181 ctgggtgcgg cctgtgagga tcagcttatc gatgataagc tgtcaaacat gagaattctt
      241 gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
      301 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
      361 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
      421 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
      481 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
      541 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
      601 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
      661 tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca
      721 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
      781 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
      841 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
      901 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
      961 acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa
     1021 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     1081 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     1141 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     1201 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     1261 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     1321 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     1381 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     1441 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     1501 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     1561 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     1621 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     1681 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     1741 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     1801 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     1861 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     1921 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     1981 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     2041 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     2101 tttgctggcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct
     2161 gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc
     2221 agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccccg agatgcgccg
     2281 cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc
     2341 attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc
     2401 gaggtgccgc cctgcttcat ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg
     2461 gaagaaatat atttgcatgt ctttagttct atgatgacac aaaccccgcc cagcgtcttg
     2521 tcattggcga attcgaacac gcagatgcag tcggggcggc gcggtccgag gtccacttcg
     2581 catattaagg tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga cccgcttaac
     2641 agcgtcaaca gcgtgccgca gatcccgggg ggcaatgaga tatgaaaaag cctgaactca
     2701 ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc
     2761 agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg
     2821 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact
     2881 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc
     2941 tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg
     3001 aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc
     3061 ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat
     3121 ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg
     3181 acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg
     3241 actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg
     3301 acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat
     3361 acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc
     3421 gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc
     3481 tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag
     3541 cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta
     3601 cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg
     3661 atagtggaaa ccgacgcccc agcactcgtc cggatcggga gatgggggag gctaactgaa
     3721 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat
     3781 aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
     3841 ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc
     3901 cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc
     3961 aggccctgcc atagccactg gccccgtggg ttagggacgg ggtcccccat ggggaatggt
     4021 ttatggttcg tgggggttat tattttgggc gttgcgtggg gtcaggtcca cgactggact
     4081 gagcagacag acccatggtt tttggatggc ctgggcatgg accgcatgta ctggcgcgac
     4141 acgaacaccg ggcgtctgtg gctgccaaac acccccgacc cccaaaaacc accgcgcgga
     4201 tttctggcgt gccaagctag tcgaccaatt ctcatgtttg acagcttatc atcgcagatc
     4261 cgggcaacgt tgttgccatt gctgcaggcg cagaactggt aggtatggaa gatctataca
     4321 ttgaatcaat attggcaatt agccatatta gtcattggtt atatagcata aatcaatatt
     4381 ggctattggc cattgcatac gttgtatcta tatcataata tgtacattta tattggctca
     4441 tgtccaatat gaccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt
     4501 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     4561 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     4621 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     4681 actgcccact tggcagtaca tcaagtgtat catatgccaa gtccgccccc tattgacgtc
     4741 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttacg ggactttcct
     4801 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     4861 tacaccaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     4921 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaat
     4981 aaccccgccc cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     5041 agagctcgtt tagtgaaccg tcagatctct agaagctggg taccagctgc tagcgccacc
     5101 atgaagacca tcatcgccct gagctacatc ttctgcctgg tgttcgccga gcagaaattg
     5161 atcagcgagg aggacctggg cagccctgga ggcagttcag gaggcggacc acattctatg
     5221 cgttatttcg agaccgccgt gtcccggccc ggcctcgggg agccccggta catctctgtc
     5281 ggctatgtgg acaacaagga gttcgtgcgc ttcgacagcg acgcggagaa tccgagatat
     5341 gagccgcagg cgccgtggat ggagcaggag gggccggagt attgggagcg gatcacgcag
     5401 atcgccaagg gccaggagca gtggttccga gtgaacctga ggaccctgct cggctactac
     5461 aaccagagcg cgggcggcac tcacacactc cagtggatgt acggctgtga cgtggggtcg
     5521 gacgggcgcc tcctccgcgg gtacgagcag ttcgcctacg acggctgcga ttacatcgcc
     5581 ctgaacgaag acctgaaaac gtggacggcg gcggacatgg cggcgcagat cacccgacgc
     5641 aagtgggagc aggctggtgc tgcagagtat tacagggcct acctggaggg cgagtgcgtg
     5701 gagtggctcc acagatacct gaagaacggg aacgcgacgc tgctgcgcac agattcccca
     5761 aaggcacatg tgacccatca ccccagatct aaaggtgaag tcaccctgag gtgctgggcc
     5821 ctgggcttct accctgctga catcaccctg acctggcagt tgaatgggga ggagctgacc
     5881 caggacatgg agcttgtgga gaccaggcct gcaggggatg gaaccttcca gaagtgggca
     5941 tctgtggtgg tgcctcttgg gaaggagcag aattacacat gccgtgtgta ccatgagggg
     6001 ctgcctgagc ccctcaccct gagatgggag cctcctccat ctacggacag ctacatggtc
     6061 atagtagctg ttcttggtgt gctcggggca atggcaatca ttggtgcagt ggtagcgttt
     6121 gtgatgaaac gccgacgaaa taccggaggt aaaggtggcg attatgcctt ggcaccaggg
     6181 agccagtcat cagagatgtc actgcgcgac tgcaaggcat gataactcga ggccggcaag
     6241 gccggatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     6301 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     6361 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     6421 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta
     6481 tgatccggct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     6541 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     6601 gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga ggtcgactct agaggatcga
     6661 tccccgcccc ggacgaacta aacctgacta cgacatctct gccccttctt cgcggggcag
     6721 tgcatgtaat cccttcagtt ggttggtaca acttgccaac tgggccctgt tccacatgtg
     6781 acacgggggg ggaccaaaca caaaggggtt ctctgactgt agttgacatc cttataaatg
     6841 gatgtgcaca tttgccaaca ctgagtggct ttcatcctgg agcagacttt gcagtctgtg
     6901 gactgcaaca caacattgcc tttatgtgta actcttggct gaagctctta caccaatgct
     6961 gggggacatg tacctcccag gggcccagga agactacggg aggctacacc aacgtcaatc
     7021 agaggggcct gtgtagctac cgataagcgg accctcaaga gggcattagc aatagtgttt
     7081 ataaggcccc cttgttaacc ctaaacgggt agcatatgct tcccgggtag tagtatatac
     7141 tatccagact aaccctaatt caatagcata tgttacccaa cgggaagcat atgctatcga
     7201 attagggtta gtaaaagggt cctaaggaac agcgatatct cccaccccat gagctgtcac
     7261 ggttttattt acatggggtc aggattccac gagggtagtg aaccatttta gtcacaaggg
     7321 cagtggctga agatcaagga gcgggcagtg aactctcctg aatcttcgcc tgcttcttca
     7381 ttct
//
