LOCUS       sequence-271181-        9765 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes CRY2-mCherry-PLD-P2A-CIBN-Sac1(TM) for mammalian expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9765)
  AUTHORS   Tei R, Baskin JM
  TITLE     Spatiotemporal control of phosphatidic acid signaling with 
            optogenetic, engineered phospholipase Ds.
  JOURNAL   J Cell Biol. 2020 Mar 2;219(3). pii: 133675. doi: 
            10.1083/jcb.201907013.
  PUBMED    31999306
REFERENCE   2  (bases 1 to 9765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-03-2"; volume: "219(3). pii: 133675. doi"; 
            pages: " 10.1083/jcb.201907013"
COMMENT     Sequence Label: Endoplasmic reticulum-targeted optoPLD
FEATURES             Location/Qualifiers
     source          1..9765
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(37..54)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(208..796)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(288..307)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(967..1758,1759..1827))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1590..1609
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1828..1932)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2007..2026)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2198..2577
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2578..2781
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2732..2752
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2783..2801
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2809..2833
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2935..4428
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWRKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             4462..5166
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(4604..4622)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4872..4891
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             6781..6837
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6844..7353
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKAAKFDTETKDCN
                     EAAKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
     primer_bind     complement(7680..7697)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7686..7910
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8043..8062)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    8194..8241
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             8249..9271
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             8249..9271
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    9401..9522
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9438..9457)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9492..9511
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9571..9587)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9571..9587)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9584..9606)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9595..9611
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9619..9625,9626..9643,9644..9649))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9664..9685
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
       61 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
      121 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
      181 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
      241 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
      301 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
      361 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
      421 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
      481 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
      541 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
      601 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
      661 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
      721 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
      781 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
      841 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
      901 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
      961 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     1021 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     1081 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     1141 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     1201 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     1261 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     1321 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     1381 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     1441 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     1501 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     1561 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     1621 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     1681 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     1741 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     1801 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     1861 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     1921 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct
     1981 cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     2041 tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct
     2101 acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt
     2161 gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat
     2221 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     2281 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     2341 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     2401 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     2461 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     2521 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     2581 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     2641 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     2701 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     2761 gaggtctata taagcagagc tctccctatc agtgatagag agtcgacgag ctcgtttagt
     2821 gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg
     2881 ggaccgatcc agcctccgga ctctagcgtt taaacttaag cttggtacgc caccatgaag
     2941 atggacaaaa agactatagt ttggtttaga agagacctaa ggattgagga taatcctgca
     3001 ttagcagcag ctgctcacga aggatctgtt tttcctgtct tcatttggtg tcctgaagaa
     3061 gaaggacagt tttatcctgg aagagcttca agatggtgga tgaaacaatc acttgctcac
     3121 ttatctcaat ccttgaaggc tcttggatct gacctcactt taatcaaaac ccacaacacg
     3181 atttcagcga tcttggattg tatccgcgtt accggtgcta caaaagtcgt ctttaaccac
     3241 ctctatgatc ctgtttcgtt agttcgggac cataccgtaa aggagaagct ggtggaacgt
     3301 gggatctctg tgcaaagcta caatggagat ctattgtatg aaccgtggga gatatactgc
     3361 gaaaagggca aaccttttac gagtttcaat tcttactgga ggaaatgctt agatatgtcg
     3421 attgaatccg ttatgcttcc tcctccttgg cggttgatgc caataactgc agcggctgaa
     3481 gcgatttggg cgtgttcgat tgaagaacta gggctggaga atgaggccga gaaaccgagc
     3541 aatgcgttgt taactagagc ttggtctcca ggatggagca atgctgataa gttactaaat
     3601 gagttcatcg agaagcagtt gatagattat gcaaagaaca gcaagaaagt tgttgggaat
     3661 tctacttcac tactttctcc gtatctccat ttcggggaaa taagcgtcag acacgttttc
     3721 cagtgtgccc ggatgaaaca aattatatgg gcaagagata agaacagtga aggagaagaa
     3781 agtgcagatc tttttcttag gggaatcggt ttaagagagt attctcggta tatatgtttc
     3841 aacttcccgt ttactcacga gcaatcgttg ttgagtcatc ttcggttttt cccttgggat
     3901 gctgatgttg ataagttcaa ggcctggaga caaggcagga ccggttatcc gttggtggat
     3961 gccggaatga gagagctttg ggctaccgga tggatgcata acagaataag agtgattgtt
     4021 tcaagctttg ctgtgaagtt tcttctcctt ccatggaaat ggggaatgaa gtatttctgg
     4081 gatacacttt tggatgctga tttggaatgt gacatccttg gctggcagta tatctctggg
     4141 agtatccccg atggccacga gcttgatcgc ttggacaatc ccgcgttaca aggcgccaaa
     4201 tatgacccag aaggtgagta cataaggcaa tggcttcccg agcttgcgag attgccaact
     4261 gaatggatcc atcatccatg ggacgctcct ttaaccgtac tcaaagcttc tggtgtggaa
     4321 ctcggaacaa actatgcgaa acccattgta gacatcgaca cagctcgtga gctactagct
     4381 aaagctattt caagaacccg tgaagcacag atcatgatcg gagcagcagg tggaggcggt
     4441 tcaggcggag gtggcagcgg cgtgagcaag ggcgaggagg ataacatggc catcatcaag
     4501 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
     4561 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     4621 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
     4681 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
     4741 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
     4801 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
     4861 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
     4921 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
     4981 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
     5041 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
     5101 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
     5161 tacaagagat ctcgaagcgc ggccgcggga gcaggaggag cagctcgagg cgcagcagcg
     5221 ggagcaggag gagcaggtcg atcgggcgga tctgcagatt cagctacacc acatttggac
     5281 gccgttgagc aaacattgag gcaagtgtcc ccaggcctgg agggcgatgt atgggaaaga
     5341 actagtggaa ataaattgga cggaagtgcc gctgatccca gtgattggtt gttgcaaacc
     5401 ccaggatgtt ggggcgatga taaatgcgct gacagagttg ggacaaaaag gttgctggct
     5461 aagatgactg aaaatatagg gaatgccacc agaaccgtag atattagtac acttgcacct
     5521 ttcccaaacg gcgcattcca agacgctatt gtcgctggtt tgaaggagtc cgcagctaaa
     5581 ggcaataagt tgaaagttag gatactggtt ggggctgcac ccgtctacca catgaatgta
     5641 ataccttcca agtataggga cgaattgact gcaaaacttg gcaaagccgc agaaaacata
     5701 actttgaacg tggctagtat gactacatca aaaacagctt tctcctggaa ccatagcaaa
     5761 atcttggttg tagacgggca aagtgccctt actggtggaa taaatagctg gaaggatgat
     5821 tacttggata ctactcatcc agtgtctgat gttgatcttg cactgaccgg accagctgca
     5881 ggctctgccg gtagatacct tgacactttg tggacatgga cctgccaaaa caagagtaac
     5941 atcgcttccg tctggtttgc agcttccggc aacgcagggt gcatgcccac tatgcataaa
     6001 gatacaaacc ccaaggcatc acctgccacc gggaatgtcc cagtaatagc cgttggaggg
     6061 ttgggcgttg gcatcaaaga tgtggacccc aagtccactt tcaggcccga tctgccaaca
     6121 gctagcgata ctaagtgtgt ggtaggactt cacgacaata ctaacgccga tagagactat
     6181 gatacagtca acccagaaga gagtgccttg agagctcttg ttgcttcagc aaaaggtcac
     6241 attgaaatat ctcagcagga tttgaacgct acatgtccac ccttgcctag gtatgacatt
     6301 agattgtacg atgccttggc tgctaaaatg gctgctgggg ttaaggtgag aattgtggtt
     6361 agcgacccag ccaacagggg cgcagtgggc agtgggggtt acagccaaat aaaatcactt
     6421 agtgaaatat cagacacttt gagaaacagg cttgcaaata tcacaggcgg tcaacaagct
     6481 gccaaaactg caatgtgtag caatttgcag cttgcaacct ttagatcctc ccccaacggc
     6541 aaatgggcag acgggcatcc ttatgcccaa caccataagt tggtttccgt cgactcatcc
     6601 acattttata tcggttcaaa aaatttgtac ccatcctggc tgcaagattt tggctacata
     6661 gtggaatccc cagaggctgc caagcaactg gatgccaaat tgcttgaccc tcagtggaag
     6721 tactcacagg agactgcaac agtggattac gcaagaggaa tctgcaacgc tggaagcggc
     6781 gctacaaact ttagtctgct caagcaggct ggcgatgtcg aggaaaaccc aggtcccggt
     6841 accatgaatg gagctatagg aggtgacctt ttgctcaatt ttcctgacat gtcggtccta
     6901 gagcgccaaa gggctcacct caagtacctc aatcccacct ttgattctcc tctcgccggc
     6961 ttctttgccg attcttcaat gattaccggc ggcgagatgg acagctatct ttcgactgcc
     7021 ggtttgaatc ttccgatgat gtacggtgag acgacggtgg aaggtgattc aagactctca
     7081 atttcgccgg aaacgacgct tgggactgga aatttcaagg cagcgaagtt tgatacagag
     7141 actaaggatt gtaatgaggc ggcgaagaag atgacgatga acagagatga cctagtagaa
     7201 gaaggagaag aagagaagtc gaaaataaca gagcaaaaca atgggagcac aaaaagcatc
     7261 aagaagatga aacacaaagc caagaaagaa gagaacaatt tctctaatga ttcatctaaa
     7321 gtgacgaagg aattggagaa aacggattat attcatgtga ccggtggcgc cttcctggct
     7381 ttgcctgtta tcatggttgt tgccttttca atgtgcatta tctgtttgct tatggctggt
     7441 gacacttgga cagaaacact ggcctatgtg ctcttctggg gagttgcaag cattggaaca
     7501 ttttttatca ttctttacaa tggcaaagat tttgtcgatg ctcccagact ggtccagaaa
     7561 gaaaagatag actgagtacc gagctcggat ccactagtcc agtgtggtgg aattctgcag
     7621 atatccagca cagtggcggc cgctcgagtc tagagggccc gtttaaaccc gctgatcagc
     7681 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     7741 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     7801 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     7861 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     7921 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     7981 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     8041 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     8101 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     8161 aaaacttgat tagggtgatg gttcacgtac ctagaagttc ctattccgaa gttcctattc
     8221 tctagaaagt ataggaactt ccttggccaa aaagcctgaa ctcaccgcga cgtctgtcga
     8281 gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga
     8341 agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag
     8401 ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct
     8461 cccgattccg gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc
     8521 ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct
     8581 gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg
     8641 gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg
     8701 cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc
     8761 gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg
     8821 gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac
     8881 agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat
     8941 cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag
     9001 gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga
     9061 ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg
     9121 atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag
     9181 aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg
     9241 ccccagcact cgtccgaggg caaaggaata gcacgtacta cgagatttcg attccaccgc
     9301 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     9361 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     9421 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     9481 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     9541 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     9601 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     9661 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     9721 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggg
//
