LOCUS       sequence-271252-        4078 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PCR-based C-terminal SRS tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Nemec AA, Tomko RJ Jr
  TITLE     A suite of PCR-based peptide tagging plasmids to permit 
            epitope-targeted enzymatic functionalization of yeast proteins.
  JOURNAL   Yeast. 2020 May 13. doi: 10.1002/yea.3471.
  PUBMED    32401365
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2020
            May 13. doi: 10.1002/yea.3471."
COMMENT     Sequence Label: pFA6a-SRS:HIS3MX6
FEATURES             Location/Qualifiers
     source          1..4078
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(23..47)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        230..573
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             574..1272
                     /codon_start=1
                     /product="S. kluyveri imidazoleglycerol phosphate 
                     dehydratase"
                     /label=k-HIS3
                     /translation="MAEPAQKKQKQTVQERKAFISRITNETKIQIAISLNGGYIQIKDS
                     ILPAKKDDDVASQATQSQVIDIHTGVGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHT
                     TEDCGIALGQAFKEAMGAVRGVKRFGTGFAPLDEALSRAVVDLSSRPFAVIDLGLKREM
                     IGDLSTEMIPHFLESFAEAARITLHVDCLRGFNDHHRSESAFKALAVAIREAISSNGTN
                     DVPSTKGVLM"
     terminator      1276..1473
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(1577..1596)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1578..1596)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1683..1700)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1854..2442)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1934..1953)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2613..3404,3405..3473))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3236..3255
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3474..3578)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3646..3664
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3702..3724)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3824..3843
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        3924..3942
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3924..3941
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      join(4046..4078,1..155)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
ORIGIN
        1 aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta ggttttaaaa
       61 cgaaaattct tattcttgag taactctttc ctgtaggtca ggttgctttc tcaggtatag
      121 tatgaggtcg ctcttattga ccacacctct accggcagat ccgctaggga taacagggta
      181 atatagatct gtttagcttg cctcgtcccc gccgggtcac ccggccagcg acatggaggc
      241 ccagaatacc ctccttgaca gtcttgacgt gcgcagctca ggggcatgat gtgactgtcg
      301 cccgtacatt tagcccatac atccccatgt ataatcattt gcatccatac attttgatgg
      361 ccgcacggcg cgaagcaaaa attacggctc ctcgctgcag acctgcgagc agggaaacgc
      421 tcccctcaca gacgcgttga attgtcccca cgccgcgccc ctgtagagaa atataaaagg
      481 ttaggatttg ccactgaggt tcttctttca tatacttcct tttaaaatct tgctaggata
      541 cagttctcac atcacatccg aacataaaca accatggcag aaccagccca aaaaaagcaa
      601 aaacaaactg ttcaggagcg caaggcgttt atctcccgta tcactaatga aactaaaatt
      661 caaatcgcta tttcgctgaa tggtggttat attcaaataa aagattcgat tcttcctgca
      721 aagaaggatg acgatgtagc ttcccaagct actcagtcac aggtcatcga tattcacaca
      781 ggtgttggct ttttggatca tatgatccat gcgttggcaa aacactctgg ttggtctctt
      841 attgttgaat gtattggtga cctgcacatt gacgatcacc atactaccga agattgcggt
      901 atcgcattag ggcaagcgtt caaagaagca atgggtgctg tccgtggtgt aaaaagattc
      961 ggtactgggt tcgcaccatt ggatgaggcg ctatcacgtg ccgtagtcga tttatctagt
     1021 agaccatttg ctgtaatcga ccttggattg aagagagaga tgattggtga tttatccact
     1081 gaaatgattc cacacttttt ggaaagtttc gcggaggcgg ccagaattac tttgcatgtt
     1141 gattgtctga gaggtttcaa cgatcaccac agaagtgaga gtgcgttcaa ggctttggct
     1201 gttgccataa gagaagctat ttctagcaat ggcaccaatg acgttccctc aaccaaaggt
     1261 gttttgatgt gaagtactga caataaaaag attcttgttt tcaagaactt gtcatttgta
     1321 tagttttttt atattgtagt tgttctattt taatcaaatg ttagcgtgat ttatattttt
     1381 tttcgcctcg acatcatctg cccagatgcg aagttaagtg cgcagaaagt aatatcatgc
     1441 gtcaatcgta tgtgaatgct ggtcgctata ctgctgtcga ttcgatacta acgccgccat
     1501 ccagtttaaa cgagctcgaa ttcatcgatg atatcagatc cactagtggc ctatgcggcc
     1561 gcggatctgc cggtctccct atagtgagtc gtattaattt cgataagcca ggttaacctg
     1621 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     1681 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     1741 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     1801 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     1861 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     1921 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     1981 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     2041 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     2101 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     2161 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     2221 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     2281 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     2341 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     2401 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     2461 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     2521 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     2581 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     2641 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     2701 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     2761 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     2821 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     2881 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     2941 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     3001 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     3061 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     3121 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     3181 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     3241 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     3301 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     3361 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     3421 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     3481 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     3541 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     3601 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     3661 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     3721 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     3781 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     3841 gtactgagag tgcaccatat ggacatattg tcgttagaac gcggctacaa ttaatacata
     3901 accttatgta tcatacacat acgatttagg tgacactata gaacgcggcc gccagctgaa
     3961 gcttcgtacg ctgcaggtcg acggatcccc gggttaatta actgcccgaa accggtggtg
     4021 gctaaggcgc gccacttcta aataagcgaa tttcttatga tttatgattt ttattatt
//
