LOCUS       sequence-271255-        7261 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV vector to drive the expression of Gq-DREADD-P2A-dTomato in PV 
            cortical interneuronsunder the control of the E2 regulatory element.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7261)
  AUTHORS   Vormstein-Schneider D, Lin JD, Pelkey KA, Chittajallu R, Guo B, 
            Arias-Garcia MA, Allaway K, Sakopoulos S, Schneider G, Stevenson O, 
            Vergara J, Sharma J, Zhang Q, Franken TP, Smith J, Ibrahim LA, M 
            Astro KJ, Sabri E, Huang S, Favuzzi E, Burbridge T, Xu Q, Guo L, 
            Vogel I, Sanchez V, Saldi GA, Gorissen BL, Yuan X, Zaghloul KA, 
            Devinsky O, Sabatini BL, Batista-Brito R, Reynolds J, Feng G, Fu Z, 
            McBain CJ, Fishell G, Dimidschstein J
  TITLE     Viral manipulation of functionally distinct interneurons in mice, 
            non-human primates and humans.
  JOURNAL   Nat Neurosci. 2020 Dec;23(12):1629-1636. doi: 
            10.1038/s41593-020-0692-9. Epub 2020 Aug 17.
  PUBMED    32807948
REFERENCE   2  (bases 1 to 7261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41593-020-0692-9"; journalName: "Nat Neurosci"; date: 
            "2020-12"; volume: "23"; issue: "12"; pages: "1629-1636"
COMMENT     Sequence Label: pAAV-S5E2-Gq-P2A-dTomato
FEATURES             Location/Qualifiers
     source          1..7261
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     intron          934..1066
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             1089..1115
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2904..2960
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2973..2993
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2994..3692
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGL
                     VTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQ
                     ALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHH
                     LFLYGMDELYK"
     primer_bind     complement(3124..3142)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3392..3411
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3586..3609
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    3747..4335
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3800..3820)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4218..4229)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4364..4445
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4401..4420)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4455..4474
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   4524..4664
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4524..4653
                     /label=AAV2 ITR
     rep_origin      4739..5194
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4826..4845)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5036..5057
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5211..5230)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5330..5352
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        5476..5580
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5581..5649,5650..6441)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5799..5818)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6612..7200
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7101..7120
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtttaatt aaactagtgg tttaatctaa catggctgct
      181 atagcttact gactagaagt taagtgcaca cttcctaaaa gaaggctttg acacaagcca
      241 cttcagttcc ctcctcattt tcttgtcccc attcctctct ctgtagaatt ctgagatttc
      301 aattcagttt tatacagaaa ccacattact gtaagcccta caaagttatg gcaatatagc
      361 tatatggagt caagtaatgt aggttatttt tttcccaatg gtgctggtga aggtggcaat
      421 tatgtagcta tacttagcag actgaggaaa ttctgctaga gtcagcattt gtctcttcat
      481 tgctatgaaa cagtaatgga aaaataaaca aaaacaaaag gcaaacacta tgcataattc
      541 cctcagatca tattaacatg tgatgttgga gtaaattgtt ataaccccat tttggaaata
      601 cttaccttaa ttaactatga tttccttaaa ataatgcagt atttacaatc tatatgaaag
      661 cactatatgg gacacatggt atgatggaac agtgcaccca agagacacca agaacattcc
      721 tgtctgtggc agtcttttct ctatacagag gcatttagtc tcaattgctc agagttattt
      781 taaacttcct gcagcccggg ctgggcataa aagtcagggc agagccatct attgcttaca
      841 tttgcttcta gcctgcaagt cgaggagcgc agccttccag aagcagagcg cggcgcctta
      901 agctgcagaa gttggtcgtg aggcactggg cacgtaagta tcaaggttac aagacaggtt
      961 taaggagacc aatagaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1021 gcacctattg gtcttactga catccacttt gcctttctct ccacagcgag ctcgctagcg
     1081 ccaccatgta cccatacgat gttccagatt acgctacctt gcacaataac agtacaacct
     1141 cgcctttgtt tccaaacatc agctcctcct ggatacacag cccctccgat gcagggctgc
     1201 ccccgggaac cgtcactcat ttcggcagct acaatgtttc tcgagcagct ggcaatttct
     1261 cctctccaga cggtaccacc gatgaccctc tgggaggtca taccgtctgg caagtggtct
     1321 tcatcgcttt cttaacgggc atcctggcct tggtgaccat catcggcaac atcctggtaa
     1381 ttgtgtcatt taaggtcaac aagcagctga agacggtcaa caactacttc ctcttaagcc
     1441 tggcctgtgc cgatctgatt atcggggtca tttcaatgaa tctgtttacg acctacatca
     1501 tcatgaatcg atgggcctta gggaacttgg cctgtgacct ctggcttgcc attgactgcg
     1561 tagccagcaa tgcctctgtt atgaatcttc tggtcatcag ctttgacaga tacttttcca
     1621 tcacgaggcc gctcacgtac cgagccaaac gaacaacaaa gagagccggt gtgatgatcg
     1681 gtctggcttg ggtcatctcc tttgtccttt gggctcctgc catcttgttc tggcaatact
     1741 ttgttggaaa gagaactgtg cctccgggag agtgcttcat tcagttcctc agtgagccca
     1801 ccattacttt tggcacagcc atcgctggtt tttatatgcc tgtcaccatt atgactattt
     1861 tatactggag gatctataag gaaactgaaa agcgtaccaa agagcttgct ggcctgcaag
     1921 cctctgggac agaggcagag acagaaaact ttgtccaccc cacgggcagt tctcgaagct
     1981 gcagcagtta cgaacttcaa cagcaaagca tgaaacgctc caacaggagg aagtatggcc
     2041 gctgccactt ctggttcaca accaagagct ggaaacccag ctccgagcag atggaccaag
     2101 accacagcag cagtgacagt tggaacaaca atgatgctgc tgcctccctg gagaactccg
     2161 cctcctccga cgaggaggac attggctccg agacgagagc catctactcc atcgtgctca
     2221 agcttccggg tcacagcacc atcctcaact ccaccaagtt accctcatcg gacaacctgc
     2281 aggtgcctga ggaggagctg gggatggtgg acttggagag gaaagccgac aagctgcagg
     2341 cccagaagag cgtggacgat ggaggcagtt ttccaaaaag cttctccaag cttcccatcc
     2401 agctagagtc agccgtggac acagctaaga cttctgacgt caactcctca gtgggtaaga
     2461 gcacggccac tctacctctg tccttcaagg aagccactct ggccaagagg tttgctctga
     2521 agaccagaag tcagatcact aagcggaaaa ggatgtccct ggtcaaggag aagaaagcgg
     2581 cccagaccct cagtgcgatc ttgcttgcct tcatcatcac ttggacccca tacaacatca
     2641 tggttctggt gaacaccttt tgtgacagct gcatacccaa aaccttttgg aatctgggct
     2701 actggctgtg ctacatcaac agcaccgtga accccgtgtg ctatgctctg tgcaacaaaa
     2761 cattcagaac cactttcaag atgctgctgc tgtgccagtg tgacaaaaaa aagaggcgca
     2821 agcagcagta ccagcagaga cagtcggtca tttttcacaa gcgcgcaccc gagcaggcct
     2881 tgtccggaga taacggaagc ggagccacta acttctccct gttgaaacaa gcaggggatg
     2941 tcgaagagaa tcccgggcca tctagaatgg cccccaagaa gaagaggaag gtggtgagca
     3001 agggcgagga ggtcatcaaa gagttcatgc gcttcaaggt gcgcatggag ggctccatga
     3061 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     3121 ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc
     3181 cccagttcat gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc cccgattaca
     3241 agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     3301 gtctggtgac cgtgacccag gactcctccc tgcaggacgg cacgctgatc tacaaggtga
     3361 agatgcgcgg caccaacttc ccccccgacg gccccgtaat gcagaagaag accatgggct
     3421 gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc gagatccacc
     3481 aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagacc atctacatgg
     3541 ccaagaagcc cgtgcaactg cccggctact actacgtgga caccaagctg gacatcacct
     3601 cccacaacga ggactacacc atcgtggaac agtacgagcg ctccgagggc cgccaccacc
     3661 tgttcctgta cggcatggac gagctgtaca agtaagtcga cggcgcgccc cacccctgca
     3721 gggaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat
     3781 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     3841 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     3901 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     3961 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     4021 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     4081 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     4141 aatcatcgtc ctttccttgg ctgctcgcct atgttgccac ctggattctg cgcgggacgt
     4201 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     4261 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     4321 gggccgcctc cccgcatcga taccgagcgc tgctcgagag atcaacttgt ttattgcagc
     4381 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     4441 actgcattct agttgtggtt tgtccaaact catcaatgta tcagcttatc gataccgcat
     4501 gcacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct
     4561 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc
     4621 ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta
     4681 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac
     4741 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     4801 acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4861 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     4921 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
     4981 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     5041 ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa
     5101 gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     5161 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc
     5221 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     5281 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     5341 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     5401 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     5461 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     5521 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     5581 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     5641 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     5701 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     5761 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     5821 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     5881 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     5941 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     6001 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     6061 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     6121 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     6181 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     6241 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     6301 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     6361 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     6421 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     6481 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     6541 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     6601 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     6661 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     6721 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     6781 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6841 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6901 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6961 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     7021 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     7081 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     7141 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     7201 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     7261 t
//
