LOCUS       sequence-271263-       11917 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Three-fragment Multisite Gateway vector construction for green 
            fluorescent seed selection in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11917)
  AUTHORS   Wang X, Ye L, Lyu M, Ursache R, Loytynoja A, Mahonen AP
  TITLE     An inducible genome editing system for plants.
  JOURNAL   Nat Plants. 2020 Jun 29. pii: 10.1038/s41477-020-0695-2. doi: 
            10.1038/s41477-020-0695-2.
  PUBMED    32601420
REFERENCE   2  (bases 1 to 11917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2020 Jun 29. pii: 10.1038/s41477-020-0695-2. doi: 
            10.1038/s41477-020-0695-2."
COMMENT     Sequence Label: pFG7m34GW
FEATURES             Location/Qualifiers
     source          1..11917
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(2142..2144,2145..2147,2148..2861)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2142..2144,2145..2147,2148..2858)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2187..2208)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2448..2467)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2795..2816
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      complement(2860..3110)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3192..3216
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             3737..4528
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4774..5362
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5263..5282
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5516..5533
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5548..5688
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5614..5633)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5774..5796
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6032..6226
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6292..7365)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(7794..8423)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    9754..9778
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     9848..9870
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9867..9883
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9867..9883
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    9903..10026
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(10067..10372)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(10169..10188)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(10692..11372)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     11287..11306
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(11426..11432,11433..11450,11451..11456))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(11481..11604)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(11612..11629)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11612..11628)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11621..11643)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cttcaagtgt atgtaggtat agtaacatga acaagaacag actcaagtac aagatcgcat
       61 acgaaaatgg aaatggcaat gtcacttcca cataatcaaa cacgaatcct catatcaaca
      121 aggcctgaga ttctaactag ctcataacaa cttagccaat agttacttga gactaccaaa
      181 tgtatgtaga actaaagact aagggacaga gagttcgtct aaacaggtga atctagtcgt
      241 tgttatctaa taaacaattc agccccaaat gcagaacaca catagagctc tctattgatt
      301 caaattacga tctgatactg ataacgtcta gatttttagg gttaaagcaa tcaatcacct
      361 gacgattcaa ggtggttgga tcatgacgat tccagaaaac atcaagcaag ctctcaaagc
      421 tacactcttt gggatcatac tgaactctaa caacctcgtt atgtcccgta gtgccagtac
      481 agacatcctc gtaactcgga ttgtgcacga tgccatggct atacccaacc tcggtcttgg
      541 tcacaccagg aactctctgg taagctagct ccactcccca gaaacaaccg gcgccaaatt
      601 gcgcgaattg ctgacctgaa gacggaacat catcgtcggg tccttgggcg attgcggcgg
      661 aagatgggtc agcttgggct tgaggacgag acccgaatcc gagtctgttg aaaaggttgt
      721 tcattgggga tttgtatacg gagattggtc gtcgagaggt ttgagggaaa ggacaaatgg
      781 gtttggctct ggagaaagag agtgcggctt tagagagaga attgagaggt ttagagagag
      841 atgcggcggc gatgagcgga ggagagacga cgaggacctg cattatcaaa gcagtgacgt
      901 ggtgaaattt ggaactttta agaggcagat agatttatta tttgtatcca ttttcttcat
      961 tgttctagaa tgtcgcggaa caaattttaa aactaaatcc taaatttttc taattttgtt
     1021 gccaatagtg gatatgtggg ccgtatagaa ggaatctatt gaaggcccaa acccatactg
     1081 acgagcccaa aggttcgttt tgcgttttat gtttcggttc gatgccaacg ccacattctg
     1141 agctaggcaa aaaacaaacg tgtctttgaa tagactcctc tcgttaacac atgcagcggc
     1201 tgcatggtga cgccattaac acgtggccta caattgcatg atgtctccat tgacacgtga
     1261 cttctcgtct cctttcttaa tatatctaac aaacactcct acctcttcca aaatatatac
     1321 acatcttttt gatcaatctc tcattcaaaa tctcattctc tctagtaaac aagaacaaaa
     1381 aaatggcgga tacagctaga ggaacccatc acgatatcat cggcagagac cagtacccga
     1441 tgatgggccg agaccgagac cagtaccaga tgtccggacg aggatctgac tactccaagt
     1501 ctaggcagat tgctaaagct gcaactgctg tcacagctgg tggttccctc cttgttctct
     1561 ccagccttac ccttgttgga actgtcatag ctttgactgt tgcaacacct ctgctcgtta
     1621 tcttcagccc aatccttgtc ccggctctca tcacagttgc actcctcatc accggttttc
     1681 tttcctctgg agggtttggc attgccgcta taaccgtttt ctcttggatt tacaagtaag
     1741 cacacattta tcatcttact tcataatttt gtgcaatatg tgcatgcatg tgttgagcca
     1801 gtagctttgg atcaattttt ttggtcgaat aacaaatgta acaataagaa attgcaaatt
     1861 ctagggaaca tttggttaac taaatacgaa atttgaccta gctagcttga atgtgtctgt
     1921 gtatatcatc tatataggta aaatgcttgg tatgatacct attgattgtg aataggtacg
     1981 caacgggaga gcacccacag ggatcagaca agttggacag tgcaaggatg aagttgggaa
     2041 gcaaagctca ggatctgaaa gacagagctc agtactacgg acagcaacat actggtgggg
     2101 aacatgaccg tgaccgtact cgtggtggcc agcacactac tatggtgagc aagggcgagg
     2161 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     2221 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     2281 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     2341 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     2401 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     2461 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     2521 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     2581 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     2641 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     2701 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     2761 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     2821 ccgccgggat cactctcggc atggacgagc tgtacaagtg atctagtaac atagatgaac
     2881 cgcgcgcgat aatttatcct agtttgcgcg ctatattttg ttttctatcg cgtattaaat
     2941 gtataattgc gggactctaa tcataaaaac ccatctcata aataacgtca tgcattacat
     3001 gttaattatt acatgcttaa cgtaattcaa cagaaattat atgataatca tcgcaagacc
     3061 ggcaacagga ttcaatctta agaaacttta ttgccaaatg tttgaacgat ggagctcgaa
     3121 ttcaattcgg cgttaattca gtacattaaa aacgtccgca atgtgttatt aagttgtcta
     3181 agcgtcaatt tgtttacacc acaatatatc ctgccaccag ccagccaaca gctccccgac
     3241 cggcagctcg gcacaaaatc accactcgat acaggcagcc catcagtccg ggacggcgtc
     3301 agcgggagag ccgttgtaag gcggcagact ttgctcatgt taccgatgct attcggaaga
     3361 acggcaacta agctgccggg tttgaaacac ggatgatctc gcggagggta gcatgttgat
     3421 tgtaacgatg acagagcgtt gctgcctgtg atcaattcgg gcacgaaccc agtggacata
     3481 agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag
     3541 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
     3601 ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt
     3661 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa
     3721 aacaaagtta aacatcatgg gggaagcggt gatcgccgaa gtatcgactc aactatcaga
     3781 ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg
     3841 ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac
     3901 cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc
     3961 ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga
     4021 catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa
     4081 tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct
     4141 gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga
     4201 tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc
     4261 gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta
     4321 cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg
     4381 cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga
     4441 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg
     4501 cgagatcacc aaggtagtcg gcaaataatg tctagctaga aattcgttca agccgacgcc
     4561 gcttcgcggc gcggcttaac tcaagcgtta gatgcactaa gcacataatt gctcacagcc
     4621 aaactatcag gtcaagtctg cttttattat ttttaagcgt gcataataag ccctacacaa
     4681 attgggagat atatcatgca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     4741 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     4801 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     4861 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     4921 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     4981 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     5041 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     5101 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     5161 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     5221 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     5281 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     5341 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     5401 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     5461 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     5521 gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg
     5581 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     5641 aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg
     5701 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     5761 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     5821 gcgaggcagg gtgccttgat gtgggcgccg gcggtcgagt ggcgacggcg cggcttgtcc
     5881 gcgccctggt agattgcctg gccgtaggcc agccattttt gagcggccag cggccgcgat
     5941 aggccgacgc gaagcggcgg ggcgtaggga gcgcagcgac cgaagggtag gcgctttttg
     6001 cagctcttcg gctgtgcgct ggccagacag ttatgcacag gccaggcggg ttttaagagt
     6061 tttaataagt tttaaagagt tttaggcgga aaaatcgcct tttttctctt ttatatcagt
     6121 cacttacatg tgtgaccggt tcccaatgta cggctttggg ttcccaatgt acgggttccg
     6181 gttcccaatg tacggctttg ggttcccaat gtacgtgcta tccacaggaa agagaccttt
     6241 tcgacctttt tcccctgcta gggcaatttg ccctagcatc tgctccgtac attaggaacc
     6301 ggcggatgct tcgccctcga tcaggttgcg gtagcgcatg actaggatcg ggccagcctg
     6361 ccccgcctcc tccttcaaat cgtactccgg caggtcattt gacccgatca gcttgcgcac
     6421 ggtgaaacag aacttcttga actctccggc gctgccactg cgttcgtaga tcgtcttgaa
     6481 caaccatctg gcttctgcct tgcctgcggc gcggcgtgcc aggcggtaga gaaaacggcc
     6541 gatgccggga tcgatcaaaa agtaatcggg gtgaaccgtc agcacgtccg ggttcttgcc
     6601 ttctgtgatc tcgcggtaca tccaatcagc tagctcgatc tcgatgtact ccggccgccc
     6661 ggtttcgctc tttacgatct tgtagcggct aatcaaggct tcaccctcgg ataccgtcac
     6721 caggcggccg ttcttggcct tcttcgtacg ctgcatggca acgtgcgtgg tgtttaaccg
     6781 aatgcaggtt tctaccaggt cgtctttctg ctttccgcca tcggctcgcc ggcagaactt
     6841 gagtacgtcc gcaacgtgtg gacggaacac gcggccgggc ttgtctccct tcccttcccg
     6901 gtatcggttc atggattcgg ttagatggga aaccgccatc agtaccaggt cgtaatccca
     6961 cacactggcc atgccggccg gccctgcgga aacctctacg tgcccgtctg gaagctcgta
     7021 gcggatcacc tcgccagctc gtcggtcacg cttcgacaga cggaaaacgg ccacgtccat
     7081 gatgctgcga ctatcgcggg tgcccacgtc atagagcatc ggaacgaaaa aatctggttg
     7141 ctcgtcgccc ttgggcggct tcctaatcga cggcgcaccg gctgccggcg gttgccggga
     7201 ttctttgcgg attcgatcag cggccgcttg ccacgattca ccggggcgtg cttctgcctc
     7261 gatgcgttgc cgctgggcgg cctgcgcggc cttcaacttc tccaccaggt catcacccag
     7321 cgccgcgccg atttgtaccg ggccggatgg tttgcgaccg ctcacgccga ttcctcgggc
     7381 ttgggggttc cagtgccatt gcagggccgg cagacaaccc agccgcttac gcctggccaa
     7441 ccgcccgttc ctccacacat ggggcattcc acggcgtcgg tgcctggttg ttcttgattt
     7501 tccatgccgc ctcctttagc cgctaaaatt catctactca tttattcatt tgctcattta
     7561 ctctggtagc tgcgcgatgt attcagatag cagctcggta atggtcttgc cttggcgtac
     7621 cgcgtacatc ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga cccgcttcat
     7681 ggctggcgtg tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg
     7741 gccggcactt agcgtgtttg tgcttttgct cattttctct ttacctcatt aactcaaatg
     7801 agttttgatt taatttcagc ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt
     7861 tctgattcaa gaacggttgt gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg
     7921 atacgggact caagaatggg cagctcgtac ccggccagcg cctcggcaac ctcaccgccg
     7981 atgcgcgtgc ctttgatcgc ccgcgacacg acaaaggccg cttgtagcct tccatccgtg
     8041 acctcaatgc gctgcttaac cagctccacc aggtcggcgg tggcccatat gtcgtaaggg
     8101 cttggctgca ccggaatcag cacgaagtcg gctgccttga tcgcggacac agccaagtcc
     8161 gccgcctggg gcgctccgtc gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg
     8221 cggtcaatcg tcgggcggtc gatgccgaca acggttagcg gttgatcttc ccgcacggcc
     8281 gcccaatcgc gggcactgcc ctggggatcg gaatcgacta acagaacatc ggccccggcg
     8341 agttgcaggg cgcgggctag atgggttgcg atggtcgtct tgcctgaccc gcctttctgg
     8401 ttaagtacag cgataacctt catgcgttcc ccttgcgtat ttgtttattt actcatcgca
     8461 tcatatacgc agcgaccgca tgacgcaagc tgttttactc aaatacacat caccttttta
     8521 gacggcggcg ctcggtttct tcagcggcca agctggccgg ccaggccgcc agcttggcat
     8581 cagacaaacc ggccaggatt tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga
     8641 acacgtaccc ggccgcgatc atctccgcct cgatctcttc ggtaatgaaa aacggttcgt
     8701 cctggccgtc ctggtgcggt ttcatgcttg ttcctcttgg cgttcattct cggcggccgc
     8761 cagggcgtcg gcctcggtca atgcgtcctc acggaaggca ccgcgccgcc tggcctcggt
     8821 gggcgtcact tcctcgctgc gctcaagtgc gcggtacagg gtcgagcgat gcacgccaag
     8881 cagtgcagcc gcctctttca cggtgcggcc ttcctggtcg atcagctcgc gggcgtgcgc
     8941 gatctgtgcc ggggtgaggg tagggcgggg gccaaacttc acgcctcggg ccttggcggc
     9001 ctcgcgcccg ctccgggtgc ggtcgatgat tagggaacgc tcgaactcgg caatgccggc
     9061 gaacacggtc aacaccatgc ggccggccgg cgtggtggtg tcggcccacg gctctgccag
     9121 gctacgcagg cccgcgccgg cctcctggat gcgctcggca atgtccagta ggtcgcgggt
     9181 gctgcgggcc aggcggtcta gcctggtcac tgtcacaacg tcgccagggc gtaggtggtc
     9241 aagcatcctg gccagctccg ggcggtcgcg cctggtgccg gtgatcttct cggaaaacag
     9301 cttggtgcag ccggccgcgt gcagttcggc ccgttggttg gtcaagtcct ggtcgtcggt
     9361 gctgacgcgg gcatagccca gcaggccagc ggcggcgctc ttgttcatgg cgtaatgtct
     9421 ccggttctag tcgcaagtat tctactttat gcgactaaaa cacgcgacaa gaaaacgcca
     9481 ggaaaagggc agggcggcag cctgtcgcgt aacttaggac ttgtgcgaca tgtcgttttc
     9541 agaagacggc tgcactgaac gtcagaagcc gactgcacta tagcagcgga ggggttggat
     9601 caaagtactt taaagtactt taaagtactt taaagtactt tgatcccgag gggaaccctg
     9661 tggttggcat gcacatacaa atggacgaac ggataaacct tttcacgccc ttttaaatat
     9721 ccgattattc taataaacgc tcttttctct taggtttacc cgccaatata tcctgtcaaa
     9781 cactgatagt ttaaactgaa ggcgggaaac gacaatctga tccaagctca agctaagctt
     9841 gggcccgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct
     9901 atcaactttg tatagaaaag ttgaacgaga aacgtaaaat gatataaata tcaatatatt
     9961 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc
    10021 actatggtcg acctgcagac tggctgtgta taagggagcc tgacatttat attccccaga
    10081 acatcaggtt aatggcgttt ttgatgtcat tttcgcggtg gctgagatca gccacttctt
    10141 ccccgataac ggagaccggc acactggcca tatcggtggt catcatgcgc cagctttcat
    10201 ccccgatatg caccaccggg taaagttcac gggggacttt atctgacagc agacgtgcac
    10261 tggccagggg gatcaccatc cgtcgcccgg gcgtgtcaat aatatcactc tgtacatcca
    10321 caaacagacg ataacggctc tctcttttat aggtgtaaac cttaaactgc atttcaccag
    10381 cccctgttct cgtcggcaaa agagccgttc atttcaataa accgggcgac ctcagccatc
    10441 ccttcttgat tttccgcttt ccagcgttcg gcacgcagac gacgggcttc attctgcatg
    10501 gttgtgctta ccgaaccgga gatactgaca tcatatatgc cttgagcaac tgatagctgt
    10561 cgctgtcaac tgtcactgta atacgctgct tcatagcata cctctttttg acatacttcg
    10621 ggtatacata tcagtatata ttcttatacc gcaaaaatca gcgcgcaaat acgcatactg
    10681 ttatctggct tttagtaagc cggatcctct agattacgcc ccgcctgcca ctcatcgcag
    10741 tactgttgta attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga
    10801 acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg
    10861 aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc
    10921 acccagggat tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc
    10981 aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg
    11041 tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa
    11101 caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat acggaattcc
    11161 ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta
    11221 tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta
    11281 cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca
    11341 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc
    11401 gacggatcct aactcaaaat ccacacatta tacgagccgg aagcataaag tgtaaagcct
    11461 ggggtgccta atgcggccgc catagtgact ggatatgttg tgttttacag tattatgtag
    11521 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
    11581 gttcaacttt attatacata gttgataatt cactggccgt cgttttacaa cgtcgtgact
    11641 gggaaaacga tctaactgac tagccgcggc catgctagag tccgcaaaaa tcaccagtct
    11701 ctctctacaa atctatctct ctctattttt ctccagaata atgtgtgagt agttcccaga
    11761 taagggaatt agggttctta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta
    11821 gtatgtattt gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaccaaa
    11881 atccagtgac ctgcaggtcg accatatggg agagctc
//
