LOCUS       sequence-271264-       11666 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Three-fragment Multisite Gateway vector construction for red 
            fluorescent seed selection in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11666)
  AUTHORS   Wang X, Ye L, Lyu M, Ursache R, Loytynoja A, Mahonen AP
  TITLE     An inducible genome editing system for plants.
  JOURNAL   Nat Plants. 2020 Jun 29. pii: 10.1038/s41477-020-0695-2. doi: 
            10.1038/s41477-020-0695-2.
  PUBMED    32601420
REFERENCE   2  (bases 1 to 11666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2020 Jun 29. pii: 10.1038/s41477-020-0695-2. doi: 
            10.1038/s41477-020-0695-2."
COMMENT     Sequence Label: pFRm43GW
FEATURES             Location/Qualifiers
     source          1..11666
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2142..2837
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHK"
     terminator      complement(2836..3087)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3183..3207
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             3728..4519
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4765..5353
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5254..5273
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5507..5524
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5539..5679
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5605..5624)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5765..5787
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6023..6217
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6283..7356)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(7785..8414)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    9745..9769
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     9843..9865
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9857..9874
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9858..9874
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9882..10005
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(10030..10035,10036..10053,10054..10060)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             10114..10794
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(10180..10199)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             11114..11419
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     11298..11317
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(11460..11583)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(11603..11619)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11603..11619)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11616..11638)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 cttcaagtgt atgtaggtat agtaacatga acaagaacag actcaagtac aagatcgcat
       61 acgaaaatgg aaatggcaat gtcacttcca cataatcaaa cacgaatcct catatcaaca
      121 aggcctgaga ttctaactag ctcataacaa cttagccaat agttacttga gactaccaaa
      181 tgtatgtaga actaaagact aagggacaga gagttcgtct aaacaggtga atctagtcgt
      241 tgttatctaa taaacaattc agccccaaat gcagaacaca catagagctc tctattgatt
      301 caaattacga tctgatactg ataacgtcta gatttttagg gttaaagcaa tcaatcacct
      361 gacgattcaa ggtggttgga tcatgacgat tccagaaaac atcaagcaag ctctcaaagc
      421 tacactcttt gggatcatac tgaactctaa caacctcgtt atgtcccgta gtgccagtac
      481 agacatcctc gtaactcgga ttgtgcacga tgccatggct atacccaacc tcggtcttgg
      541 tcacaccagg aactctctgg taagctagct ccactcccca gaaacaaccg gcgccaaatt
      601 gcgcgaattg ctgacctgaa gacggaacat catcgtcggg tccttgggcg attgcggcgg
      661 aagatgggtc agcttgggct tgaggacgag acccgaatcc gagtctgttg aaaaggttgt
      721 tcattgggga tttgtatacg gagattggtc gtcgagaggt ttgagggaaa ggacaaatgg
      781 gtttggctct ggagaaagag agtgcggctt tagagagaga attgagaggt ttagagagag
      841 atgcggcggc gatgagcgga ggagagacga cgaggacctg cattatcaaa gcagtgacgt
      901 ggtgaaattt ggaactttta agaggcagat agatttatta tttgtatcca ttttcttcat
      961 tgttctagaa tgtcgcggaa caaattttaa aactaaatcc taaatttttc taattttgtt
     1021 gccaatagtg gatatgtggg ccgtatagaa ggaatctatt gaaggcccaa acccatactg
     1081 acgagcccaa aggttcgttt tgcgttttat gtttcggttc gatgccaacg ccacattctg
     1141 agctaggcaa aaaacaaacg tgtctttgaa tagactcctc tcgttaacac atgcagcggc
     1201 tgcatggtga cgccattaac acgtggccta caattgcatg atgtctccat tgacacgtga
     1261 cttctcgtct cctttcttaa tatatctaac aaacactcct acctcttcca aaatatatac
     1321 acatcttttt gatcaatctc tcattcaaaa tctcattctc tctagtaaac aagaacaaaa
     1381 aaatggcgga tacagctaga ggaacccatc acgatatcat cggcagagac cagtacccga
     1441 tgatgggccg agaccgagac cagtaccaga tgtccggacg aggatctgac tactccaagt
     1501 ctaggcagat tgctaaagct gcaactgctg tcacagctgg tggttccctc cttgttctct
     1561 ccagccttac ccttgttgga actgtcatag ctttgactgt tgcaacacct ctgctcgtta
     1621 tcttcagccc aatccttgtc ccggctctca tcacagttgc actcctcatc accggttttc
     1681 tttcctctgg agggtttggc attgccgcta taaccgtttt ctcttggatt tacaagtaag
     1741 cacacattta tcatcttact tcataatttt gtgcaatatg tgcatgcatg tgttgagcca
     1801 gtagctttgg atcaattttt ttggtcgaat aacaaatgta acaataagaa attgcaaatt
     1861 ctagggaaca tttggttaac taaatacgaa atttgaccta gctagcttga atgtgtctgt
     1921 gtatatcatc tatataggta aaatgcttgg tatgatacct attgattgtg aataggtacg
     1981 caacgggaga gcacccacag ggatcagaca agttggacag tgcaaggatg aagttgggaa
     2041 gcaaagctca ggatctgaaa gacagagctc agtactacgg acagcaacat actggtgggg
     2101 aacatgaccg tgaccgtact cgtggtggcc agcacactac tatgagcgag ctgattaagg
     2161 agaacatgca catgaagctg tacatggagg gcaccgtgaa caaccaccac ttcaagtgca
     2221 catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc aaggtggtcg
     2281 agggcggccc tctccccttc gccttcgaca tcctggctac cagcttcatg tacggcagca
     2341 gaaccttcat caaccacacc cagggcatcc ccgacttctt taagcagtcc ttccctgagg
     2401 gcttcacatg ggagagagtc accacatacg aagacggggg cgtgctgacc gctacccagg
     2461 acaccagcct ccaggacggc tgcctcatct acaacgtcaa gatcagaggg gtgaacttcc
     2521 catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggccaac accgagatgc
     2581 tgtaccccgc tgacggcggc ctggaaggca gaagcgacat ggccctgaag ctcgtgggcg
     2641 ggggccacct gatctgcaac ttcaagacca catacagatc caagaaaccc gctaagaacc
     2701 tcaagatgcc cggcgtctac tatgtggacc acagactgga aagaatcaag gaggccgaca
     2761 aagagaccta cgtcgagcag cacgaggtgg ctgtggccag atactgcgac ctccctagca
     2821 aactggggca caagtgatct agtaacatag atgacaccgc gcgcgataat ttatcctagt
     2881 ttgcgcgcta tattttgttt tctatcgcgt attaaatgta taattgcggg actctaatca
     2941 taaaaaccca tctcataaat aacgtcatgc attacatgtt aattattaca tgcttaacgt
     3001 aattcaacag aaattatatg ataatcatcg caagaccggc aacaggattc aatcttaaga
     3061 aactttattg ccaaatgttt gaacgatggt accctgcatg caagctgata attcaattcg
     3121 gcgttaattc agtacattaa aaacgtccgc aatgtgttat taagttgtct aagcgtcaat
     3181 ttgtttacac cacaatatat cctgccacca gccagccaac agctccccga ccggcagctc
     3241 ggcacaaaat caccactcga tacaggcagc ccatcagtcc gggacggcgt cagcgggaga
     3301 gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc tattcggaag aacggcaact
     3361 aagctgccgg gtttgaaaca cggatgatct cgcggagggt agcatgttga ttgtaacgat
     3421 gacagagcgt tgctgcctgt gatcaattcg ggcacgaacc cagtggacat aagcctgttc
     3481 ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac
     3541 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt
     3601 tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg
     3661 tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt
     3721 aaacatcatg ggggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg
     3781 cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt
     3841 ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct
     3901 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg
     3961 agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc
     4021 gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct
     4081 tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc
     4141 aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc
     4201 tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga
     4261 ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt
     4321 aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc
     4381 ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg
     4441 cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac
     4501 caaggtagtc ggcaaataat gtctagctag aaattcgttc aagccgacgc cgcttcgcgg
     4561 cgcggcttaa ctcaagcgtt agatgcacta agcacataat tgctcacagc caaactatca
     4621 ggtcaagtct gcttttatta tttttaagcg tgcataataa gccctacaca aattgggaga
     4681 tatatcatgc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     4741 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     4801 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     4861 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     4921 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     4981 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     5041 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     5101 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     5161 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     5221 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     5281 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     5341 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     5401 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     5461 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     5521 cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     5581 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     5641 tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc
     5701 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     5761 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag
     5821 ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg
     5881 tagattgcct ggccgtaggc cagccatttt tgagcggcca gcggccgcga taggccgacg
     5941 cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt gcagctcttc
     6001 ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg gttttaagag ttttaataag
     6061 ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct tttatatcag tcacttacat
     6121 gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg tacgggttcc ggttcccaat
     6181 gtacggcttt gggttcccaa tgtacgtgct atccacagga aagagacctt ttcgaccttt
     6241 ttcccctgct agggcaattt gccctagcat ctgctccgta cattaggaac cggcggatgc
     6301 ttcgccctcg atcaggttgc ggtagcgcat gactaggatc gggccagcct gccccgcctc
     6361 ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca
     6421 gaacttcttg aactctccgg cgctgccact gcgttcgtag atcgtcttga acaaccatct
     6481 ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag agaaaacggc cgatgccggg
     6541 atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat
     6601 ctcgcggtac atccaatcag ctagctcgat ctcgatgtac tccggccgcc cggtttcgct
     6661 ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg gataccgtca ccaggcggcc
     6721 gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt
     6781 ttctaccagg tcgtctttct gctttccgcc atcggctcgc cggcagaact tgagtacgtc
     6841 cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc ttcccttccc ggtatcggtt
     6901 catggattcg gttagatggg aaaccgccat cagtaccagg tcgtaatccc acacactggc
     6961 catgccggcc ggccctgcgg aaacctctac gtgcccgtct ggaagctcgt agcggatcac
     7021 ctcgccagct cgtcggtcac gcttcgacag acggaaaacg gccacgtcca tgatgctgcg
     7081 actatcgcgg gtgcccacgt catagagcat cggaacgaaa aaatctggtt gctcgtcgcc
     7141 cttgggcggc ttcctaatcg acggcgcacc ggctgccggc ggttgccggg attctttgcg
     7201 gattcgatca gcggccgctt gccacgattc accggggcgt gcttctgcct cgatgcgttg
     7261 ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc
     7321 gatttgtacc gggccggatg gtttgcgacc gctcacgccg attcctcggg cttgggggtt
     7381 ccagtgccat tgcagggccg gcagacaacc cagccgctta cgcctggcca accgcccgtt
     7441 cctccacaca tggggcattc cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg
     7501 cctcctttag ccgctaaaat tcatctactc atttattcat ttgctcattt actctggtag
     7561 ctgcgcgatg tattcagata gcagctcggt aatggtcttg ccttggcgta ccgcgtacat
     7621 cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg acccgcttca tggctggcgt
     7681 gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt gcgctcggac ggccggcact
     7741 tagcgtgttt gtgcttttgc tcattttctc tttacctcat taactcaaat gagttttgat
     7801 ttaatttcag cggccagcgc ctggacctcg cgggcagcgt cgccctcggg ttctgattca
     7861 agaacggttg tgccggcggc ggcagtgcct gggtagctca cgcgctgcgt gatacgggac
     7921 tcaagaatgg gcagctcgta cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg
     7981 cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg
     8041 cgctgcttaa ccagctccac caggtcggcg gtggcccata tgtcgtaagg gcttggctgc
     8101 accggaatca gcacgaagtc ggctgccttg atcgcggaca cagccaagtc cgccgcctgg
     8161 ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc
     8221 gtcgggcggt cgatgccgac aacggttagc ggttgatctt cccgcacggc cgcccaatcg
     8281 cgggcactgc cctggggatc ggaatcgact aacagaacat cggccccggc gagttgcagg
     8341 gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca
     8401 gcgataacct tcatgcgttc cccttgcgta tttgtttatt tactcatcgc atcatatacg
     8461 cagcgaccgc atgacgcaag ctgttttact caaatacaca tcaccttttt agacggcggc
     8521 gctcggtttc ttcagcggcc aagctggccg gccaggccgc cagcttggca tcagacaaac
     8581 cggccaggat ttcatgcagc cgcacggttg agacgtgcgc gggcggctcg aacacgtacc
     8641 cggccgcgat catctccgcc tcgatctctt cggtaatgaa aaacggttcg tcctggccgt
     8701 cctggtgcgg tttcatgctt gttcctcttg gcgttcattc tcggcggccg ccagggcgtc
     8761 ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac
     8821 ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc
     8881 cgcctctttc acggtgcggc cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc
     8941 cggggtgagg gtagggcggg ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc
     9001 gctccgggtg cggtcgatga ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt
     9061 caacaccatg cggccggccg gcgtggtggt gtcggcccac ggctctgcca ggctacgcag
     9121 gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt aggtcgcggg tgctgcgggc
     9181 caggcggtct agcctggtca ctgtcacaac gtcgccaggg cgtaggtggt caagcatcct
     9241 ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc tcggaaaaca gcttggtgca
     9301 gccggccgcg tgcagttcgg cccgttggtt ggtcaagtcc tggtcgtcgg tgctgacgcg
     9361 ggcatagccc agcaggccag cggcggcgct cttgttcatg gcgtaatgtc tccggttcta
     9421 gtcgcaagta ttctacttta tgcgactaaa acacgcgaca agaaaacgcc aggaaaaggg
     9481 cagggcggca gcctgtcgcg taacttagga cttgtgcgac atgtcgtttt cagaagacgg
     9541 ctgcactgaa cgtcagaagc cgactgcact atagcagcgg aggggttgga tcaaagtact
     9601 ttaaagtact ttaaagtact ttaaagtact ttgatcccga ggggaaccct gtggttggca
     9661 tgcacataca aatggacgaa cggataaacc ttttcacgcc cttttaaata tccgattatt
     9721 ctaataaacg ctcttttctc ttaggtttac ccgccaatat atcctgtcaa acactgatag
     9781 tttaaactga aggcgggaaa cgacaatctg atccaagctc aagctaagct tgagctcgtt
     9841 ttcccagtca cgacgttgta aaacgacggc cagtgaatta tcaactatgt ataataaagt
     9901 tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa
     9961 acagactaca taatactgta aaacacaaca tatccagtca ctatggcggc cgcattaggc
    10021 accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt gagttaggat
    10081 ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
    10141 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
    10201 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag
    10261 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
    10321 catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
    10381 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
    10441 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa
    10501 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc
    10561 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc
    10621 gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt
    10681 caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa
    10741 cagtactgcg atgagtggca ggcggggcgt aatctagagg atccggctta ctaaaagcca
    10801 gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt
    10861 atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag tgacagttga
    10921 cagcgacagc tatcagttgc tcaaggcata tatgatgtca gtatctccgg ttcggtaagc
    10981 acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcaa
    11041 gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc cgacgagaac
    11101 aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct
    11161 gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct
    11221 ggccagtgca cgtctgctgt cagataaagt cccccgtgaa ctttacccgg tggtgcatat
    11281 cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat
    11341 cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct
    11401 gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc
    11461 atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct
    11521 aatttaatat attgatattt atatcatttt acgtttctcg ttcaactttt ctatacaaag
    11581 ttgatagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctcg
    11641 ggccctctag aggatccccg ggtacc
//
