LOCUS       sequence-271265-       11261 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gene knockdown for actinomycetes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11261)
  AUTHORS   Tong Y, Charusanti P, Zhang L, Weber T, Lee SY
  TITLE     CRISPR-Cas9 Based Engineering of Actinomycetal Genomes.
  JOURNAL   ACS Synth Biol. 2015 Sep 18;4(9):1020-9. doi: 
            10.1021/acssynbio.5b00038. Epub 2015 Apr 7.
  PUBMED    25806970
REFERENCE   2  (bases 1 to 11261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00038"; journalName: "ACS Synth Biol"; date: 
            "2015-09-18- 18"; volume: "4"; issue: "9"; pages: "1020-9"
COMMENT     Sequence Label: pCRISPR-dCas9
FEATURES             Location/Qualifiers
     source          1..11261
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     112..129
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    246..267
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(282..287,288..305,306..312)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    320..336
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     325..347
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     344..360
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     344..360
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_RNA        498..573
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      complement(743..777)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             join(945..971,972..974,975..3461,3462..3464,3465..5051)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     terminator      complement(5238..5286)
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             7001..8443
                     /codon_start=1
                     /gene="rep"
                     /product="replication initiator protein from the 
                     Streptomyces ghanaensis plasmid pSG5"
                     /label=pSG5 Rep
                     /translation="MWKPGEATWGNTCRCNNVHTCPWCMSRILAVRGSNVQLAADGLAD
                     AGYGLHLGTNTLRHFERMAFGTVRKGMRHGLVAVLHDGWKGAYGSSGRRWRTMRDDFGI
                     IGYERAFEDTFGWGSGWHLHWHTLWVTREVLGPDAQAAFRDALAGAWAAGVESAGGYTV
                     SETCDRPGCSCEGKGHGTDVRPLNGADAADGDAGKQARYLYKDGDKTKGGVAKIGLELA
                     GQNFKAGRGDDRMGPLDLGDAAAAELQRLRRPGPFVEKYREREFGVFQVRKHYRSQNLN
                     RLIKELGIQQDVRTEEEITDDTEGLVAIAVIPAYIWYRYIARVAGRRLDLIKVAETYGL
                     PGVRRLVESWGLVWGKDVLDPPAPEAPAAPGDLDADQMRFEVMSEEEAAFREARRKANE
                     ARTEELAASLDRVRQPKKEAIRPTISLRKRLKPKPVTVDVKTPPPGAASPVCRRCKGKL
                     APVLQPWGQHPGDCLRVDTAVA"
     oriT            9105..9214
                     /note="incP origin of transfer"
     CDS             9530..10330
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDSFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELLAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      10632..11219
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11120..11139
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
       61 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
      121 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
      181 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
      241 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
      301 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
      361 atgattacga attgtacgcg gtcgatcttg acggctggcg agaggtgcgg ggaggatctg
      421 accgacgcgg tccacacgtg gcaccgcgat gctgttgtgg gcacaatcgt gccggttggt
      481 aggatcgacg gccatgggtt ttagagctag aaatagcaag ttaaaataag gctagtccgt
      541 tatcaacttg aaaaagtggc accgagtcgg tgcttttttt acgtaggcgt cagctacgat
      601 gttccgggga ctgctgatcc ggtcagcagg tggaagaggg actggattcc aaagttctca
      661 atgctgcttg ctgttcttga atggggggtc gttgacgacg acatggctcg attggcgcga
      721 caagttgctg cgattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa
      781 atccagatgg agttctgagg tcattactgg accggatcgg ggatctgggc tgagggagcc
      841 gacggcacgc ggcggctcac ggcgtggcac gcggaacgtc cgggcttgca cctcacgtca
      901 cgtgaggagg cagcgtggac ggcgtcagag aagggagcgg acatatggac aagaagtact
      961 ccatcggcct cgcgatcggc accaactccg tgggctgggc ggtcatcacc gacgagtaca
     1021 aggtcccctc caagaagttc aaggtcctgg gcaacaccga ccggcactcg atcaagaaga
     1081 acctgatcgg cgccctgctc ttcgacagcg gcgagaccgc cgaggcgacc cgcctgaagc
     1141 ggaccgcgcg tcgccgctac acccggcgca agaaccgcat ctgctacctg caggaaatct
     1201 tctccaacga gatggccaag gtggacgact cgttcttcca ccgcctggag gagagcttcc
     1261 tggtggagga ggacaagaag cacgagcgcc acccgatctt cggcaacatc gtggacgagg
     1321 tggcctacca cgagaagtac cccaccatct accacctccg caagaagctg gtggactcga
     1381 ccgacaaggc ggacctgcgg ctcatctacc tggccctcgc gcacatgatc aagttccgcg
     1441 gccacttcct catcgagggc gacctgaacc cggacaactc cgacgtggac aagctcttca
     1501 tccagctggt gcagacctac aaccagctgt tcgaggagaa ccccatcaac gccagcggcg
     1561 tggacgccaa ggcgatcctc tccgcgcgcc tgagcaagtc ccggcgcctg gagaacctca
     1621 tcgcccagct gccgggcgag aagaagaacg gcctcttcgg caacctgatc gcgctgtcgc
     1681 tcggcctgac ccccaacttc aagagcaact tcgacctggc cgaggacgcg aagctccagc
     1741 tgtccaagga cacctacgac gacgacctgg acaacctgct cgcccagatc ggcgaccagt
     1801 acgcggacct cttcctggcc gcgaagaacc tctcggacgc catcctgctc agcgacatcc
     1861 tgcgggtcaa caccgagatc accaaggccc cgctgtcggc gagcatgatc aagcggtacg
     1921 acgagcacca ccaggacctg accctgctca aggccctcgt gcgccagcag ctgcccgaga
     1981 agtacaagga aatcttcttc gaccagtcca agaacggcta cgccggctac atcgacggcg
     2041 gcgcgtcgca ggaggagttc tacaagttca tcaagccgat cctggagaag atggacggca
     2101 ccgaggagct gctcgtcaag ctgaaccgcg aggacctgct ccgcaagcag cggaccttcg
     2161 acaacggctc catcccgcac cagatccacc tgggcgagct ccacgccatc ctccggcgcc
     2221 aggaggactt ctaccccttc ctgaaggaca accgcgagaa gatcgagaag atcctgacct
     2281 tccgcatccc gtactacgtc ggccccctgg cccgcggcaa ctcccggttc gcgtggatga
     2341 cccggaagtc ggaggagacc atcaccccgt ggaacttcga ggaggtcgtg gacaagggcg
     2401 cgtccgcgca gtcgttcatc gagcgcatga ccaacttcga caagaacctc ccgaacgaga
     2461 aggtcctgcc caagcactcc ctgctctacg agtacttcac cgtgtacaac gagctgacca
     2521 aggtcaagta cgtgaccgag ggcatgcgga agccggcctt cctgtcgggc gagcagaaga
     2581 aggcgatcgt ggacctgctc ttcaagacca accgcaaggt caccgtgaag cagctgaagg
     2641 aggactactt caagaagatc gagtgcttcg actccgtcga gatcagcggc gtggaggacc
     2701 gcttcaacgc ctccctgggc acctaccacg acctgctcaa gatcatcaag gacaaggact
     2761 tcctcgacaa cgaggagaac gaggacatcc tggaggacat cgtcctcacc ctgaccctct
     2821 tcgaggaccg cgagatgatc gaggagcggc tcaagaccta cgcccacctg ttcgacgaca
     2881 aggtgatgaa gcagctgaag cgtcgccgct acaccggctg gggccgcctc tcccggaagc
     2941 tgatcaacgg catccgggac aagcagagcg gcaagaccat cctggacttc ctcaagtccg
     3001 acggcttcgc caaccgcaac ttcatgcagc tcatccacga cgacagcctg accttcaagg
     3061 aggacatcca gaaggcccag gtctcgggcc agggcgacag cctccacgag cacatcgcca
     3121 acctggcggg ctccccggcg atcaagaagg gcatcctcca gaccgtcaag gtcgtggacg
     3181 agctggtcaa ggtgatgggc cgccacaagc ccgagaacat cgtgatcgag atggcccggg
     3241 agaaccagac cacccagaag ggccagaaga actcgcgcga gcggatgaag cggatcgagg
     3301 agggcatcaa ggagctcggc agccagatcc tgaaggagca cccggtcgag aacacccagc
     3361 tgcagaacga gaagctgtac ctctactacc tgcagaacgg ccgcgacatg tacgtggacc
     3421 aggagctcga catcaaccgg ctgtccgact acgacgtgga cgcgatcgtg ccgcagtcct
     3481 tcctgaagga cgactcgatc gacaacaagg tcctgacccg ctcggacaag aaccggggca
     3541 agtccgacaa cgtgccctcg gaggaggtcg tgaagaagat gaagaactac tggcgccagc
     3601 tgctcaacgc caagctcatc acccagcgca agttcgacaa cctgaccaag gccgagcggg
     3661 gcggcctgag cgagctcgac aaggcgggct tcatcaagcg ccagctggtc gagacccggc
     3721 agatcaccaa gcacgtggcc cagatcctgg actcccggat gaacaccaag tacgacgaga
     3781 acgacaagct gatccgcgag gtcaaggtga tcaccctcaa gagcaagctg gtctccgact
     3841 tccgcaagga cttccagttc tacaaggtcc gggagatcaa caactaccac cacgcccacg
     3901 acgcgtacct gaacgccgtc gtgggcaccg cgctgatcaa gaagtacccg aagctggagt
     3961 ccgagttcgt ctacggcgac tacaaggtct acgacgtgcg caagatgatc gccaagagcg
     4021 agcaggagat cggcaaggcc accgcgaagt acttcttcta ctccaacatc atgaacttct
     4081 tcaagaccga gatcaccctg gccaacggcg agatccgcaa gcggcccctg atcgagacca
     4141 acggcgagac cggcgagatc gtctgggaca agggccgcga cttcgccacc gtccggaagg
     4201 tgctgtcgat gccgcaggtc aacatcgtga agaagaccga ggtgcagacc ggcggcttca
     4261 gcaaggagtc catcctcccc aagcgcaaca gcgacaagct gatcgcccgg aagaaggact
     4321 gggacccgaa gaagtacggc ggcttcgaca gccccaccgt cgcctactcc gtgctggtcg
     4381 tggcgaaggt cgagaagggc aagagcaaga agctgaagtc cgtgaaggag ctgctcggca
     4441 tcaccatcat ggagcgctcc tcgttcgaga agaacccgat cgacttcctg gaggccaagg
     4501 gctacaagga ggtcaagaag gacctcatca tcaagctgcc caagtacagc ctgttcgagc
     4561 tggagaacgg ccgcaagcgg atgctcgcct ccgcgggcga gctgcagaag ggcaacgagc
     4621 tggccctccc gtcgaagtac gtcaacttcc tgtacctcgc gtcccactac gagaagctga
     4681 agggctcgcc cgaggacaac gagcagaagc agctcttcgt ggagcagcac aagcactacc
     4741 tggacgagat catcgagcag atcagcgagt tcagcaagcg cgtcatcctg gccgacgcga
     4801 acctcgacaa ggtgctgtcc gcctacaaca agcaccgcga caagccgatc cgggagcagg
     4861 cggagaacat catccacctg ttcaccctca ccaacctggg cgcccccgcc gcgttcaagt
     4921 acttcgacac caccatcgac cgcaagcggt acacctccac caaggaggtc ctcgacgcga
     4981 ccctgatcca ccagagcatc accggcctgt acgagacccg catcgacctg tcccagctcg
     5041 gcggcgactg aaagctttct agaggatccc cgggtaccga gctcgaattc cccagatcta
     5101 aagttttgtc gtctttccag acgttagtaa atgaattttc tgtatgaggt tttgctaaac
     5161 aactttcaac agtttcagcg gagtgagaat agaaaggaac aactaaagga attgcgaata
     5221 ataatttttt cacgttgaaa atctccaaaa aaaaaggctc caaaaggagc ctttaattgt
     5281 atcggtttat cagcttgctt tcgaggtgaa tttcttaaac agcttgatac cgatagttgc
     5341 gccgacaatg acaacaacca tcgcccacgc ataaccgata tattcggtcg ctgaggcttg
     5401 cagggagtca aaggccgctt ttgcgggatc tcgtcgaagg cactagaggc ctatatgcgg
     5461 ggatcgaccg cgcgggtccc ggacggggaa gagcggggag ctttgccaga gagcgacgac
     5521 ttccccttgc gttggtgatt gccggtcagg gcagccatcc gccatcgtcg cgtagggtgt
     5581 cacaccccag gaatcgcgtc actgaacaca gcagccggta ggacgaccat gactgagttg
     5641 gacaccatcg caaatccgtc cgatcccgcg gtgcagcgga tcatcgatgt caccaagccg
     5701 tcgcgatcca acataaagac aacgttgatc gaggacgtcg agcccctcat gcacagcatc
     5761 gcggccgggg tggagttcat cgaggtctac ggcagcgaca gcagtccttt tccatctgag
     5821 ttgctggatc tgtgcgggcg gcagaacata ccggtccgcc tcatcgactc ctcgatcgtc
     5881 aaccagttgt tcaaggggga gcggaaggcc aagacattcg gcatcgcccg cgtccctcgc
     5941 ccggccaggt tcggcgatat cgcgagccgg cgtggggacg tcgtcgttct cgacggggtg
     6001 aagatcgtcg ggaacatcgg cgcgatagta cgcacgtcgc tcgcgctcgg agcgtcgggg
     6061 atcatcctgg tcgacagtga catcaccagc atcgcggacc ggcgtctcca aagggccagc
     6121 cgaggttacg tcttctccct tcccgtcgtt ctctccggtc gcgaggaggc catcgccttc
     6181 attcgggaca gcggtatgca gctgatgacg ctcaaggcgg atggcgacat ttccgtgaag
     6241 gaactcgggg acaatccgga tcggctggcc ttgctgttcg gcagcgaaaa gggtgggcct
     6301 tccgacctgt tcgaggaggc gtcttccgcc tcggtttcca tccccatgat gagccagacc
     6361 gagtctctca acgtttccgt ttccctcgga atcgcgctgc acgagaggat cgacaggaat
     6421 ctcgcggcca accgataagc gcctctgttc ctcggacgct cggttgctcg acctcgattc
     6481 gtcagtgatg atcaacagga cccagaaatg gcacgagccc gggattggcc tcccgggctc
     6541 gccgcccccg aaccggcgca gcatgccgag tgaggacgtt tgctctgtga cgagcgtacg
     6601 agagaacagc gcgtcgacac caccgcgccc cgccggaccg aagtgccact gcggcacccc
     6661 gatcgtccac atccccggga agcgccccaa aatctactgc tcggacgcct gccgaaagcg
     6721 cgcaaagcgt ctcattgcgg accagtcacg cagcgtagca agcacgacta cagcgggtaa
     6781 tccggacgcg ggctttagta gtaagggaaa atctccgtcc actgcaggtc agagcgctgt
     6841 aactggaacg ggttctaccc caaacgaggg tgccgcgaca gccgggcggg aggacccgcg
     6901 cgacgagcgg ttcgcccggc gggaccggca ccagacggtg tcgctgaacg aggcgttcaa
     6961 gggctgcggg acccggctca ctgccgggac ggccgagctg atgtggaagc cgggcgaggc
     7021 gacgtggggc aacacgtgcc gctgcaacaa cgtccacacc tgcccgtggt gcatgagccg
     7081 catcctggcc gtacggggct cgaacgtgca gctcgccgcc gacggtctcg cggacgccgg
     7141 gtacgggctg caccttggca cgaacacgtt gcgccacttc gagcgcatgg cgttcggcac
     7201 cgtccgcaag ggaatgcggc acggcctggt cgcggtcttg cacgacggct ggaagggcgc
     7261 gtacggctcg tccggccgcc gctggcgcac gatgcgcgac gacttcggga tcatcggcta
     7321 cgagcgggcc ttcgaggaca ccttcggctg gggctccggc tggcacctgc actggcacac
     7381 gctctgggtc acccgcgagg tgctcggccc ggacgcccag gccgcgttcc gcgacgcact
     7441 cgccggagcc tgggccgccg gggtcgaatc cgcgggcggc tacaccgtca gcgagacgtg
     7501 cgaccggccc ggttgctcct gtgagggcaa gggccacggc accgacgtgc gcccactcaa
     7561 cggcgcggac gccgctgacg gcgatgcggg caagcaggcc cgctacctct acaaggacgg
     7621 cgacaagacc aagggcggcg tcgccaagat cggcctggag ctggccggac agaacttcaa
     7681 ggccggtcgc ggcgacgacc gcatgggacc gctcgacctg ggcgacgcgg cggccgccga
     7741 gctgcagcgg cttcgtcggc ccggcccgtt cgtcgagaag taccgcgagc gcgagttcgg
     7801 cgtcttccag gtccgcaagc actaccggtc gcagaacctg aaccggctga tcaaggaact
     7861 cggcatccag caggacgtcc gcaccgagga ggagatcacc gacgacaccg agggcctggt
     7921 cgccatcgcg gtcatcccgg cctacatctg gtaccgatac atcgcccgcg tcgccggacg
     7981 tcgcctcgac ctcatcaagg tcgccgagac gtacggccta ccgggcgtcc ggcggctcgt
     8041 cgagtcctgg ggcctggtgt ggggcaagga cgtcctggac ccgcccgccc cggaagcgcc
     8101 ggccgcgccg ggcgaccttg acgctgacca gatgaggttc gaggtcatgt ccgaggagga
     8161 agctgctttc cgcgaggcgc gccggaaggc caacgaggcc cgcacggagg agctggccgc
     8221 ttcgctcgac cgagtgcgtc agccgaagaa ggaggcgatc cgcccgacga tcagccttcg
     8281 gaagcgcctg aagccgaagc cggtcaccgt cgacgtcaag actcccccgc ccggcgccgc
     8341 gtcgcccgtg tgccgccgct gcaagggcaa gctcgccccg gtactccagc cctggggcca
     8401 gcaccccgga gactgcctcc gcgtcgacac agcggtcgcg tagggccgct gagggaccgc
     8461 catgagaccg ccaccccgga catccgggtg gcggtctcat ggcggtcccg gcgcgaaagc
     8521 cgagggtctg cctgccgtga ggtggccggc ggacctgggt tactgctgga atcccgcgcc
     8581 cagtccgagc gtccgccggc gcttttcacc tggctccggg tgctcgtcga tgagcaccag
     8641 gccgtcgacg acgacgcgtc cgcctgcctg cttttggccg gcgggccggg ccgcggttgt
     8701 ccgccgggtc ggaccggtcc cttggtcgac cgatgcctcg gccacgacga tgctgggccc
     8761 gaagcggtac gcgacccgtg ccgggccgca gcggatcgcg gcagcaggag ctccccgccc
     8821 gccacggctg gctggatggt cgcctcacgc gcccgcaggg gtgacggcgt gggtgcggcg
     8881 gtcagccccg tgatcgggcg tcagacggcc gctgagggac cgcgaccacc cctcccttcc
     8941 cgcctgccgt ctttcaccgc ccggccggtc cgcagacgcc gtacggccag gccacggagg
     9001 attacgggca gatggcgccc acccggcacc cgggctgggg caccctggac agcacgaagg
     9061 tccgccccac tcaccggggc gggccttcgc gttgagctag agttcccgcc agcctcgcag
     9121 agcaggattc ccgttgagca ccgccaggtg cgaataaggg acagtgaaga aggaacaccc
     9181 gctcgcgggt gggcctactt cacctatcct gcccggctga cgccgttgga tacaccaagg
     9241 aaagtctaca cgaacccttt ggcaaaatcc tgtatatcgt gcgaaaaagg atggatatac
     9301 cgaaaaaatc gctataatga ccccgaagca gggttatgca gcggaaaatg cagctcacgg
     9361 taactgatgc cgtatttgca gtaccagcgt acggcccaca gaatgatgtc acgctgaaaa
     9421 tgccggcctt tgaatgggtt catgtgcagc tccataagca aaaggggatg ataagtttat
     9481 caccaccgac tatttgcaac agtgccgttg atcgtgctat gatcgactga tgtcatcagc
     9541 ggtggagtgc aatgtcgtgc aatacgaatg gcgaaaagcc gagctcatcg gtcagcttct
     9601 caaccttggg gttacccccg gcggtgtgct gctggtccac agctccttcc gtagcgtccg
     9661 gcccctcgaa gatgggccac ttggactgat cgaggccctg cgtgctgcgc tgggtccggg
     9721 agggacgctc gtcatgccct cgtggtcagg tctggacgac gagccgttcg atcctgccac
     9781 gtcgcccgtt acaccggacc ttggagttgt ctctgactca ttctggcgcc tgccaaatgt
     9841 aaagcgcagc gcccatccat ttgcctttgc ggcagcgggg ccacaggcag agcagatcat
     9901 ctctgatcca ttgcccctgc cacctcactc gcctgcaagc ccggtcgccc gtgtccatga
     9961 actcgatggg caggtacttc tcctcggcgt gggacacgat gccaacacga cgctgcatct
    10021 tgccgagttg ttggcaaagg ttccctatgg ggtgccgaga cactgcacca ttcttcagga
    10081 tggcaagttg gtacgcgtcg attatctcga gaatgaccac tgctgtgagc gctttgcctt
    10141 ggcggacagg tggctcaagg agaagagcct tcagaaggaa ggtccagtcg gtcatgcctt
    10201 tgctcggttg atccgctccc gcgacattgt ggcgacagcc ctgggtcaac tgggccgaga
    10261 tccgttgatc ttcctgcatc cgccagaggc gggatgcgaa gaatgcgatg ccgctcgcca
    10321 gtcgattggc tgagctcata agttcctatt ccgaagttcc tattcgaaat gaccgaccaa
    10381 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
    10441 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
    10501 ctggagttct tcgcccaccc caaaaggatc taggtgaaga tcctttttga taatctcatg
    10561 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
    10621 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
    10681 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
    10741 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
    10801 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
    10861 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
    10921 ttaccggata aggcgcagcg gtcgggctga acggggggtc gtgcacacag cccagcttgg
    10981 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    11041 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    11101 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    11161 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    11221 acgccagcaa cgcggccttt ttacggttcc tggccttttg c
//
