LOCUS       sequence-271266-       10003 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter vector expression firefly luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10003)
  AUTHORS   Ou W, Marino MP, Suzuki A, Joshi B, Husain SR, Maisner A, Galanis E,
            Puri RK, Reiser J
  TITLE     Specific targeting of human interleukin (IL)-13 receptor 
            alpha2-positive cells with lentiviral vectors displaying IL-13.
  JOURNAL   Hum Gene Ther Methods. 2012 Apr;23(2):137-47. Epub 2012 May 21.
  PUBMED    22612657
REFERENCE   2  (bases 1 to 10003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther Methods."; date: "2012-04"; volume: "23(2)"; pages: "137-47. 
            Epub 2012 May 21"
COMMENT     Sequence Label: pNL(CMV)Fluc/CMV/WPREdU3
FEATURES             Location/Qualifiers
     source          1..10003
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      538..547
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             544..2196
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     misc_feature    2203..2791
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2256..2276)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2674..2685)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2866..3099
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(4739..4756)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4910..5498)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4990..5009)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5669..6460,6461..6529))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6292..6311
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6530..6634)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6702..6720
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6758..6780)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6880..6899
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     LTR             8052..8232
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      8859..8994
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    9272..9505
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9690..9734
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagccgcc
      541 accatggaag atgccaaaaa cattaagaag ggcccagcgc cattctaccc actcgaagac
      601 gggaccgccg gcgagcagct gcacaaagcc atgaagcgct acgccctggt gcccggcacc
      661 atcgccttta ccgacgcaca tatcgaggtg gacattacct acgccgagta cttcgagatg
      721 agcgttcggc tggcagaagc tatgaagcgc tatgggctga atacaaacca tcggatcgtg
      781 gtgtgcagcg agaatagctt gcagttcttc atgcccgtgt tgggtgccct gttcatcggt
      841 gtggctgtgg ccccagctaa cgacatctac aacgagcgcg agctgctgaa cagcatgggc
      901 atcagccagc ccaccgtcgt attcgtgagc aagaaagggc tgcaaaagat cctcaacgtg
      961 caaaagaagc taccgatcat acaaaagatc atcatcatgg atagcaagac cgactaccag
     1021 ggcttccaaa gcatgtacac cttcgtgact tcccatttgc cacccggctt caacgagtac
     1081 gacttcgtgc ccgagagctt cgaccgggac aaaaccatcg ccctgatcat gaacagtagt
     1141 ggcagtaccg gattgcccaa gggcgtagcc ctaccgcacc gcaccgcttg tgtccgattc
     1201 agtcatgccc gcgaccccat cttcggcaac cagatcatcc ccgacaccgc tatcctcagc
     1261 gtggtgccat ttcaccacgg cttcggcatg ttcaccacgc tgggctactt gatctgcggc
     1321 tttcgggtcg tgctcatgta ccgcttcgag gaggagctat tcttgcgcag cttgcaagac
     1381 tataagattc aatctgccct gctggtgccc acactattta gcttcttcgc taagagcact
     1441 ctcatcgaca agtacgacct aagcaacttg cacgagatcg ccagcggcgg ggcgccgctc
     1501 agcaaggagg taggtgaggc cgtggccaaa cgcttccacc taccaggcat ccgccagggc
     1561 tacggcctga cagaaacaac cagcgccatt ctgatcaccc ccgaagggga cgacaagcct
     1621 ggcgcagtag gcaaggtggt gcccttcttc gaggctaagg tggtggactt ggacaccggt
     1681 aagacactgg gtgtgaacca gcgcggcgag ctgtgcgtcc gtggccccat gatcatgagc
     1741 ggctacgtta acaaccccga ggctacaaac gctctcatcg acaaggacgg ctggctgcac
     1801 agcggcgaca tcgcctactg ggacgaggac gagcacttct tcatcgtgga ccggctgaag
     1861 agcctgatca aatacaaggg ctaccaggta gccccagccg aactggagag catcctgctg
     1921 caacacccca acatcttcga cgccggggtc gccggcctgc ccgacgacga tgccggcgag
     1981 ctgcccgccg cagtcgtcgt gctggaacac ggtaaaacca tgaccgagaa ggagatcgtg
     2041 gactatgtgg ccagccaggt tacaaccgcc aagaagctgc gcggtggtgt tgtgttcgtg
     2101 gacgaggtgc ctaaaggact gaccggcaag ttggacgccc gcaagatccg cgagattctc
     2161 attaaggcca agaagggcgg caagatcgcc gtgtaactcg agaatcaacc tctggattac
     2221 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     2281 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     2341 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     2401 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     2461 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     2521 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     2581 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
     2641 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     2701 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     2761 agtcggatct ccctttgggc cgcctccccg cctgggtacc tttaagacca atgacttaca
     2821 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     2881 actcccaaag aagacaagat ctgctttttg cctgtactgg gtctctctgg ttagaccaga
     2941 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     3001 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     3061 ccctcagacc cttttagtca gtgtggaaaa tctctagcac ccaggaggta gaggttgcag
     3121 tgagccaaga tcgcgccact gcattccagc ctgggcaaga aaacaagact gtctaaaata
     3181 ataataataa gttaagggta ttaaatatat ttatacatgg aggtcataaa aatatatata
     3241 tttgggctgg gcgcagtggc tcacacctgc gcccggccct ttgggaggcc gaggcaggtg
     3301 gatcacctga gtttgggagt tccagaccag cctgaccaac atggagaaac cccttctctg
     3361 tgtattttta gtagatttta ttttatgtgt attttattca caggtatttc tggaaaactg
     3421 aaactgtttt tcctctactc tgataccaca agaatcatca gcacagagga agacttctgt
     3481 gatcaaatgt ggtgggagag ggaggttttc accagcacat gagcagtcag ttctgccgca
     3541 gactcggcgg gtgtccttcg gttcagttcc aacaccgcct gcctggagag aggtcagacc
     3601 acagggtgag ggctcagtcc ccaagacata aacacccaag acataaacac ccaacaggtc
     3661 caccccgcct gctgcccagg cagagccgat tcaccaagac gggaattagg atagagaaag
     3721 agtaagtcac acagagccgg ctgtgcggga gaacggagtt ctattatgac tcaaatcagt
     3781 ctccccaagc attcggggat cagagttttt aaggataact tagtgtgtag ggggccagtg
     3841 agttggagat gaaagcgtag ggagtcgaag gtgtcctttt gcgccgagtc agttcctggg
     3901 tgggggccac aagatcggat gagccagttt atcaatccgg gggtgccagc tgatccatgg
     3961 agtgcagggt ctgcaaaata tctcaagcac tgattgatct taggttttac aatagtgatg
     4021 ttaccccagg aacaatttgg ggaaggtcag aatcttgtag cctgtagctg catgactcct
     4081 aaaccataat ttcttttttg tttttttttt tttatttttg agacagggtc tcactctgtc
     4141 acctaggctg gagtgcagtg gtgcaatcac agctcactgc agcctcaacg tcgtaagctc
     4201 aagcgatcct cccacctcag cctgcctggt agctgagact acaagcgacg ccccagttaa
     4261 tttttgtatt tttggtagag gcagcgtttt gccgtgtggc cctggctggt ctcgaactcc
     4321 tgggctcaag tgatccagcc tcagcctccc aaagtgctgg gacaaccggg gccagtcact
     4381 gcacctggcc ctaaaccata atttctaatc ttttggctaa tttgttagtc ctacaaaggc
     4441 agtctagtcc ccaggcaaaa agggggtttg tttcgggaaa gggctgttac tgtctttgtt
     4501 tcaaactata aactaagttc ctcctaaact tagttcggcc tacacccagg aatgaacaag
     4561 gagagcttgg aggttagaag cacgatggaa ttggttaggt cagatctctt tcactgtctg
     4621 agttataatt ttgcaatggt ggttcaaaga ctgcccgctt ctgacaccag tcgctgcatt
     4681 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     4741 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     4801 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     4861 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     4921 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     4981 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     5041 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     5101 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     5161 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     5221 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     5281 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     5341 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     5401 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     5461 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     5521 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     5581 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     5641 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     5701 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     5761 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     5821 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     5881 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     5941 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     6001 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     6061 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     6121 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     6181 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     6241 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     6301 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     6361 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     6421 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     6481 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     6541 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     6601 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     6661 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     6721 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     6781 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     6841 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
     6901 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     6961 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     7021 cttcgctatt acgccagggg aggcagagat tgcagtaagc tgagatcgca gcactgcact
     7081 ccagcctggg cgacagagta agactctgtc tcaaaaataa aataaataaa tcaatcagat
     7141 attccaatct tttcctttat ttatttattt attttctatt ttggaaacac agtccttcct
     7201 tattccagaa ttacacatat attctatttt tctttatatg ctccagtttt ttttagacct
     7261 tcacctgaaa tgtgtgtatc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
     7321 agcagctaac aatgctgctt gtgcctggct agaagcacaa gaggaggaag aggtgggttt
     7381 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     7441 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaaa gaagacaaga
     7501 tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc agaactacac
     7561 accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     7621 tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt
     7681 gagcctgcat ggaatggatg accctgagag agaagtgtta gagtggaggt ttgacagccg
     7741 cctagcattt catcacgtgg cccgagagct gcatccggag tatctagatg gagttccgcg
     7801 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     7861 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     7921 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc
     7981 ccgttgacgc aaatgggcgg taggcgtgta ctctagatgc tacatataag cagctgcttt
     8041 ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     8101 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgctcaaa gtagtgtgtg
     8161 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     8221 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagtaaagc cagaggagat
     8281 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
     8341 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
     8401 cgtcggtatt aagcggggga gaattagata aatgggaaaa aattcggtta aggccagggg
     8461 gaaagaaaca atataaacta aaacatatag tatgggcaag cagggagcta gaacgattcg
     8521 cagttaatcc tggcctttta gagacatcag aaggctgtag acaaatactg ggacagctac
     8581 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca atagcagtcc
     8641 tctattgtgt gcatcaaagg atagatgtaa aagacaccaa ggaagcctta gataagatag
     8701 aggaagagca aaacaaaagt aagaaaaagg cacagcaagc agcagctgac acaggaaaca
     8761 acagccaggt cagccaaaat taccctatag tgcagaacct ccaggggcaa atggtacatc
     8821 aggccatatc acctagaact ttaaatatga ctccgcccat cccgccccta actccgccca
     8881 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     8941 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     9001 tttgcaaatg cataaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     9061 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     9121 aaattttcgg gtttattaca gggacagcag agatccacaa ttggagaagt gaattatata
     9181 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     9241 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     9301 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     9361 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     9421 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     9481 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     9541 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaata
     9601 acatgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     9661 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     9721 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     9781 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctgtag
     9841 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaatcccga
     9901 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     9961 ccattcgatt agtgaacgga tctagttaac tagatctaga gtc
//
