LOCUS       sequence-271268-       11302 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter vector encoding firefly luciferase and TurboRFP fluorescent
            protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11302)
  AUTHORS   Gills J, Moret R, Zhang X, Nelson J, Maresh G, Hellmers L, Canter D,
            Hudson M, Halat S, Matrana M, Marino MP, Reiser J, Shuh M, Laborde 
            E, Latsis M, Talwar S, Bardot S, Li L
  TITLE     A patient-derived orthotopic xenograft model enabling human 
            high-grade urothelial cell carcinoma of the bladder tumor 
            implantation, growth, angiogenesis, and metastasis.
  JOURNAL   Oncotarget. 2018 Aug 24;9(66):32718-32729. doi: 
            10.18632/oncotarget.26024. eCollection 2018 Aug 24.
  PUBMED    30220977
REFERENCE   2  (bases 1 to 11302)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.18632/oncotarget.26024"; journalName: "Oncotarget"; date: 
            "2018-08-24- 24"; volume: "9"; issue: "66"; pages: "32718-32729"
COMMENT     Sequence Label: pNL(CMV)Luc2-Turbo RFP/CMV/WPREdU3
FEATURES             Location/Qualifiers
     source          1..11302
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     634..650
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     634..650
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        673..691
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     673..690
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    716..740
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      852..861
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2520..2561
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2580..2645
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     protein_bind    complement(3369..3393)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(3424..3443)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3425..3443)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3454..3471)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3454..3470)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3463..3485)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    3502..4090
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3555..3575)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3973..3984)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4165..4398
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6038..6055)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6209..6797)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6289..6308)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6968..7759,7760..7828))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7591..7610
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7829..7933)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8001..8019
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8057..8079)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8179..8198
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     LTR             9351..9531
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      10158..10293
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    10571..10804
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10989..11033
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct
      601 agcctaggcc gcgggacgga taacaatttc acacaggaaa cagctatgac cattaggcct
      661 ttgcaaaaag ctatttaggt gacactatag aaggtacgcc tgcaggtacc ggatcacaag
      721 tttgtacaaa aaagcaggct ggcgccggaa ccaattcagt cgactggatc cggtacctga
      781 gctcgctagc ctcgaggata tcaagatctg gcctcggcgg ccaagcttgg caatccggta
      841 ctgttggtaa agccaccatg gaagatgcca aaaacattaa gaagggccca gcgccattct
      901 acccactcga agacgggacc gccggcgagc agctgcacaa agccatgaag cgctacgccc
      961 tggtgcccgg caccatcgcc tttaccgacg cacatatcga ggtggacatt acctacgccg
     1021 agtacttcga gatgagcgtt cggctggcag aagctatgaa gcgctatggg ctgaatacaa
     1081 accatcggat cgtggtgtgc agcgagaata gcttgcagtt cttcatgccc gtgttgggtg
     1141 ccctgttcat cggtgtggct gtggccccag ctaacgacat ctacaacgag cgcgagctgc
     1201 tgaacagcat gggcatcagc cagcccaccg tcgtattcgt gagcaagaaa gggctgcaaa
     1261 agatcctcaa cgtgcaaaag aagctaccga tcatacaaaa gatcatcatc atggatagca
     1321 agaccgacta ccagggcttc caaagcatgt acaccttcgt gacttcccat ttgccacccg
     1381 gcttcaacga gtacgacttc gtgcccgaga gcttcgaccg ggacaaaacc atcgccctga
     1441 tcatgaacag tagtggcagt accggattgc ccaagggcgt agccctaccg caccgcaccg
     1501 cttgtgtccg attcagtcat gcccgcgacc ccatcttcgg caaccagatc atccccgaca
     1561 ccgctatcct cagcgtggtg ccatttcacc acggcttcgg catgttcacc acgctgggct
     1621 acttgatctg cggctttcgg gtcgtgctca tgtaccgctt cgaggaggag ctattcttgc
     1681 gcagcttgca agactataag attcaatctg ccctgctggt gcccacacta tttagcttct
     1741 tcgctaagag cactctcatc gacaagtacg acctaagcaa cttgcacgag atcgccagcg
     1801 gcggggcgcc gctcagcaag gaggtaggtg aggccgtggc caaacgcttc cacctaccag
     1861 gcatccgcca gggctacggc ctgacagaaa caaccagcgc cattctgatc acccccgaag
     1921 gggacgacaa gcctggcgca gtaggcaagg tggtgccctt cttcgaggct aaggtggtgg
     1981 acttggacac cggtaaaaca ctgggcgtga accagcgagg cgaactgtgc gtcagaggac
     2041 ctatgattat gtcaggctat gtgaacaacc ccgaagcaac aaacgccctg atcgataagg
     2101 acggatggct gcacagcggg gatattgctt actgggatga ggacgaacat ttctttatcg
     2161 tcgaccgact gaagagtctg attaagtaca aaggatatca ggtggcacca gccgagctgg
     2221 aatcaatcct gctgcagcac cccaatattt tcgatgctgg agtggcagga ctgccagacg
     2281 atgacgcagg agagctgcct gccgctgtgg tcgtgctgga acatggcaag actatgaccg
     2341 agaaagaaat cgtcgactat gtggcatctc aggtgaccac agccaagaaa ctgagaggcg
     2401 gagtcgtgtt tgtcgatgag gtgcctaagg ggctgaccgg caaactggac gctaggaaga
     2461 tcagagaaat cctgatcaag gcaaagaaag gaggcaagat cgccgtccgg gctaagcgag
     2521 gcaaacccat tcctaaccca ctgctgggac tggatagcac atccggatct ggggcacccg
     2581 tgaaacagac tctgaatttc gatctgctga agctggctgg cgacgtggag tcaaaccctg
     2641 gacctactat gagcgaactg attaaggaga atatgcacat gaagctgtac atggagggca
     2701 ccgtgaacaa ccaccacttc aagtgcacat ccgagggcga aggcaagccc tacgagggca
     2761 cccagaccat gaagatcaag gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc
     2821 tggctaccag cttcatgtac ggcagcaaag ccttcatcaa ccacacccag ggcatccccg
     2881 acttctttaa gcagtccttc cctgagggct tcacatggga gagaatcacc acatacgaag
     2941 acgggggcgt gctgaccgct acccaggaca ccagcttcca gaacggctgc atcatctaca
     3001 acgtcaagat caacggggtg aacttcccat ccaacggccc tgtgatgcag aagaaaacac
     3061 gcggctggga ggccaacacc gagatgctgt accccgctga cggcggcctg agaggccaca
     3121 gccagatggc cctgaagctc gtgggcgggg gctacctgca ctgctccttc aagaccacat
     3181 acagatccaa gaaacccgct aagaacctca agatgcccgg cttccacttc gtggaccaca
     3241 gactggaaag aatcaaggag gccgacaaag agacctacgt cgagcagcac gagatggctg
     3301 tggccaagta ctgcgacctc cctagcaaac tggggcacag atgatgcggc cgcactcgag
     3361 atatctagac ccagctttct tgtacaaagt ggtgatcgcg tgcatgcgac gtcatagctc
     3421 tctccctata gtgagtcgta ttataagcta ggcactggcc gtcgttttac aacgtcgtga
     3481 ctgggaaaac tgctagtcga gaatcaacct ctggattaca aaatttgtga aagattgact
     3541 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     3601 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     3661 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     3721 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     3781 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     3841 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
     3901 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     3961 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     4021 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     4081 gcctccccgc ctgggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     4141 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaaaga agacaagatc
     4201 tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     4261 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     4321 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     4381 tgtggaaaat ctctagcacc caggaggtag aggttgcagt gagccaagat cgcgccactg
     4441 cattccagcc tgggcaagaa aacaagactg tctaaaataa taataataag ttaagggtat
     4501 taaatatatt tatacatgga ggtcataaaa atatatatat ttgggctggg cgcagtggct
     4561 cacacctgcg cccggccctt tgggaggccg aggcaggtgg atcacctgag tttgggagtt
     4621 ccagaccagc ctgaccaaca tggagaaacc ccttctctgt gtatttttag tagattttat
     4681 tttatgtgta ttttattcac aggtatttct ggaaaactga aactgttttt cctctactct
     4741 gataccacaa gaatcatcag cacagaggaa gacttctgtg atcaaatgtg gtgggagagg
     4801 gaggttttca ccagcacatg agcagtcagt tctgccgcag actcggcggg tgtccttcgg
     4861 ttcagttcca acaccgcctg cctggagaga ggtcagacca cagggtgagg gctcagtccc
     4921 caagacataa acacccaaga cataaacacc caacaggtcc accccgcctg ctgcccaggc
     4981 agagccgatt caccaagacg ggaattagga tagagaaaga gtaagtcaca cagagccggc
     5041 tgtgcgggag aacggagttc tattatgact caaatcagtc tccccaagca ttcggggatc
     5101 agagttttta aggataactt agtgtgtagg gggccagtga gttggagatg aaagcgtagg
     5161 gagtcgaagg tgtccttttg cgccgagtca gttcctgggt gggggccaca agatcggatg
     5221 agccagttta tcaatccggg ggtgccagct gatccatgga gtgcagggtc tgcaaaatat
     5281 ctcaagcact gattgatctt aggttttaca atagtgatgt taccccagga acaatttggg
     5341 gaaggtcaga atcttgtagc ctgtagctgc atgactccta aaccataatt tcttttttgt
     5401 tttttttttt ttatttttga gacagggtct cactctgtca cctaggctgg agtgcagtgg
     5461 tgcaatcaca gctcactgca gcctcaacgt cgtaagctca agcgatcctc ccacctcagc
     5521 ctgcctggta gctgagacta caagcgacgc cccagttaat ttttgtattt ttggtagagg
     5581 cagcgttttg ccgtgtggcc ctggctggtc tcgaactcct gggctcaagt gatccagcct
     5641 cagcctccca aagtgctggg acaaccgggg ccagtcactg cacctggccc taaaccataa
     5701 tttctaatct tttggctaat ttgttagtcc tacaaaggca gtctagtccc caggcaaaaa
     5761 gggggtttgt ttcgggaaag ggctgttact gtctttgttt caaactataa actaagttcc
     5821 tcctaaactt agttcggcct acacccagga atgaacaagg agagcttgga ggttagaagc
     5881 acgatggaat tggttaggtc agatctcttt cactgtctga gttataattt tgcaatggtg
     5941 gttcaaagac tgcccgcttc tgacaccagt cgctgcatta atgaatcggc caacgcgcgg
     6001 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     6061 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     6121 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     6181 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     6241 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     6301 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     6361 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     6421 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     6481 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     6541 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     6601 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     6661 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     6721 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     6781 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     6841 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     6901 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     6961 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7021 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     7081 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     7141 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     7201 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     7261 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     7321 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     7381 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     7441 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     7501 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     7561 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     7621 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     7681 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     7741 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     7801 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     7861 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     7921 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     7981 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
     8041 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     8101 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     8161 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     8221 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca
     8281 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagggga
     8341 ggcagagatt gcagtaagct gagatcgcag cactgcactc cagcctgggc gacagagtaa
     8401 gactctgtct caaaaataaa ataaataaat caatcagata ttccaatctt ttcctttatt
     8461 tatttattta ttttctattt tggaaacaca gtccttcctt attccagaat tacacatata
     8521 ttctattttt ctttatatgc tccagttttt tttagacctt cacctgaaat gtgtgtatcg
     8581 agacctagaa aaacatggag caatcacaag tagcaataca gcagctaaca atgctgcttg
     8641 tgcctggcta gaagcacaag aggaggaaga ggtgggtttt ccagtcacac ctcaggtacc
     8701 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     8761 gggactggaa gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta
     8821 ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag gggtcagata
     8881 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
     8941 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg gaatggatga
     9001 ccctgagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
     9061 ccgagagctg catccggagt atctagatgg agttccgcgt tacataactt acggtaaatg
     9121 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     9181 ccatagtaac gccaataggg actttccatt gacgtcaatg ggagtttgtt ttggcaccaa
     9241 aatcaacggg actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt
     9301 aggcgtgtac tctagatgct acatataagc agctgctttt tgcctgtact gggtctctct
     9361 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     9421 ctcaataaag cttgccttga gtgctcaaag tagtgtgtgc ccgtctgttg tgtgactctg
     9481 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     9541 gaacagggac ttgaaagcga aagtaaagcc agaggagatc tctcgacgca ggactcggct
     9601 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     9661 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcggtatta agcgggggag
     9721 aattagataa atgggaaaaa attcggttaa ggccaggggg aaagaaacaa tataaactaa
     9781 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggccttttag
     9841 agacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     9901 cagaagaact tagatcatta tataatacaa tagcagtcct ctattgtgtg catcaaagga
     9961 tagatgtaaa agacaccaag gaagccttag ataagataga ggaagagcaa aacaaaagta
    10021 agaaaaaggc acagcaagca gcagctgaca caggaaacaa cagccaggtc agccaaaatt
    10081 accctatagt gcagaacctc caggggcaaa tggtacatca ggccatatca cctagaactt
    10141 taaatatgac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
    10201 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
    10261 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaatgc ataaaagaaa
    10321 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
    10381 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
    10441 ggacagcaga gatccacaat tggagaagtg aattatataa atataaagta gtaaaaattg
    10501 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
    10561 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
    10621 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
    10681 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
    10741 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
    10801 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
    10861 ctagttggag taataaatct ctggaacaga tttggaataa catgacctgg atggagtggg
    10921 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
    10981 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
    11041 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
    11101 tggtaggttt aagaatagtt tttgctgtac tttctgtagt gaatagagtt aggcagggat
    11161 attcaccatt atcgtttcag acccacctcc caatcccgag gggacccgac aggcccgaag
    11221 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
    11281 ctagttaact agatctagag tc
//
