LOCUS       sequence-271270-        9487 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter vector encoding Gaussia luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9487)
  AUTHORS   Ou W, Marino MP, Suzuki A, Joshi B, Husain SR, Maisner A, Galanis E,
            Puri RK, Reiser J
  TITLE     Specific targeting of human interleukin (IL)-13 receptor 
            alpha2-positive cells with lentiviral vectors displaying IL-13.
  JOURNAL   Hum Gene Ther Methods. 2012 Apr;23(2):137-47. Epub 2012 May 21.
  PUBMED    22612657
REFERENCE   2  (bases 1 to 9487)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther Methods."; date: "2012-04"; volume: "23(2)"; pages: "137-47. 
            Epub 2012 May 21"
COMMENT     Sequence Label: pNL(CMV)GLuc/CMV/WPREdU3
FEATURES             Location/Qualifiers
     source          1..9487
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     634..650
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     634..650
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        673..691
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     673..690
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    716..740
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1554..1578)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(1609..1628)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1610..1628)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1639..1656)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1639..1655)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1648..1670)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    1687..2275
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1740..1760)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2158..2169)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2350..2583
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(4223..4240)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4394..4982)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4474..4493)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5153..5944,5945..6013))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5776..5795
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6014..6118)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6186..6204
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6242..6264)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6364..6383
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     LTR             7536..7716
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      8343..8478
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    8756..8989
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9174..9218
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct
      601 agcctaggcc gcgggacgga taacaatttc acacaggaaa cagctatgac cattaggcct
      661 ttgcaaaaag ctatttaggt gacactatag aaggtacgcc tgcaggtacc ggatcacaag
      721 tttgtacaaa aaagcaggct ggcgccggaa ccaattcagt cgactggatc cggtaccatc
      781 atggccctgc ccgtgacagc tctgctgctg cctctggccc tgctgctgca tgccgccaga
      841 cccgggatcc aaatgaagcc caccgagaac aacgaagact tcaacatcgt ggccgtggcc
      901 agcaacttcg cgaccacgga tctcgatgct gaccgcggga agttgcccgg caagaagctg
      961 ccgctggagg tgctcaaaga gatggaagcc aatgcccgga aagctggctg caccaggggc
     1021 tgtctgatct gcctgtccca catcaagtgc acgcccaaga tgaagaagtt catcccagga
     1081 cgctgccaca cctacgaagg cgacaaagag tccgcacagg gcggcatagg cgaggcgatc
     1141 gtcgacatcc ccgagatccc cggcttcaag gacctggaac ccatggaaca gtttatcgcc
     1201 caggtggacc tgtgcgtgga ctgcaccacc ggctgtctga agggcctggc caacgtgcag
     1261 tgcagcgacc tgctgaagaa gtggctgccc cagagatgcg ccaccttcgc cagcaagatc
     1321 cagggccagg tggacaagat caagggcgct ggcggcgacg cttgtagacc tgccgctggc
     1381 ggagccgtgc acaccagagg cctggacttc gcctgcgaca tctacatctg ggcccctctg
     1441 gccggaacat gtggcgtgct gctgctgagc ctggtcatca ccctgtactg caaccaccgg
     1501 aaccggcgga gagtgtgcaa gtgcccctga gcggccgcac tcgagatatc tagacccagc
     1561 tttcttgtac aaagtggtga tcgcgtgcat gcgacgtcat agctctctcc ctatagtgag
     1621 tcgtattata agctaggcac tggccgtcgt tttacaacgt cgtgactggg aaaactgcta
     1681 gtcgagaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     1741 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     1801 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     1861 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     1921 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     1981 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     2041 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
     2101 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     2161 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     2221 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctggg
     2281 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     2341 aggggggact ggaagggcta attcactccc aaagaagaca agatctgctt tttgcctgta
     2401 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     2461 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     2521 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     2581 gcacccagga ggtagaggtt gcagtgagcc aagatcgcgc cactgcattc cagcctgggc
     2641 aagaaaacaa gactgtctaa aataataata ataagttaag ggtattaaat atatttatac
     2701 atggaggtca taaaaatata tatatttggg ctgggcgcag tggctcacac ctgcgcccgg
     2761 ccctttggga ggccgaggca ggtggatcac ctgagtttgg gagttccaga ccagcctgac
     2821 caacatggag aaaccccttc tctgtgtatt tttagtagat tttattttat gtgtatttta
     2881 ttcacaggta tttctggaaa actgaaactg tttttcctct actctgatac cacaagaatc
     2941 atcagcacag aggaagactt ctgtgatcaa atgtggtggg agagggaggt tttcaccagc
     3001 acatgagcag tcagttctgc cgcagactcg gcgggtgtcc ttcggttcag ttccaacacc
     3061 gcctgcctgg agagaggtca gaccacaggg tgagggctca gtccccaaga cataaacacc
     3121 caagacataa acacccaaca ggtccacccc gcctgctgcc caggcagagc cgattcacca
     3181 agacgggaat taggatagag aaagagtaag tcacacagag ccggctgtgc gggagaacgg
     3241 agttctatta tgactcaaat cagtctcccc aagcattcgg ggatcagagt ttttaaggat
     3301 aacttagtgt gtagggggcc agtgagttgg agatgaaagc gtagggagtc gaaggtgtcc
     3361 ttttgcgccg agtcagttcc tgggtggggg ccacaagatc ggatgagcca gtttatcaat
     3421 ccgggggtgc cagctgatcc atggagtgca gggtctgcaa aatatctcaa gcactgattg
     3481 atcttaggtt ttacaatagt gatgttaccc caggaacaat ttggggaagg tcagaatctt
     3541 gtagcctgta gctgcatgac tcctaaacca taatttcttt tttgtttttt tttttttatt
     3601 tttgagacag ggtctcactc tgtcacctag gctggagtgc agtggtgcaa tcacagctca
     3661 ctgcagcctc aacgtcgtaa gctcaagcga tcctcccacc tcagcctgcc tggtagctga
     3721 gactacaagc gacgccccag ttaatttttg tatttttggt agaggcagcg ttttgccgtg
     3781 tggccctggc tggtctcgaa ctcctgggct caagtgatcc agcctcagcc tcccaaagtg
     3841 ctgggacaac cggggccagt cactgcacct ggccctaaac cataatttct aatcttttgg
     3901 ctaatttgtt agtcctacaa aggcagtcta gtccccaggc aaaaaggggg tttgtttcgg
     3961 gaaagggctg ttactgtctt tgtttcaaac tataaactaa gttcctccta aacttagttc
     4021 ggcctacacc caggaatgaa caaggagagc ttggaggtta gaagcacgat ggaattggtt
     4081 aggtcagatc tctttcactg tctgagttat aattttgcaa tggtggttca aagactgccc
     4141 gcttctgaca ccagtcgctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     4201 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     4261 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     4321 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     4381 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     4441 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     4501 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     4561 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     4621 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     4681 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     4741 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     4801 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     4861 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     4921 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4981 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     5041 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     5101 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     5161 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     5221 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     5281 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     5341 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     5401 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     5461 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     5521 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     5581 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     5641 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     5701 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     5761 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     5821 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     5881 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     5941 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     6001 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     6061 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     6121 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     6181 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     6241 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     6301 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     6361 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     6421 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt
     6481 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca ggggaggcag agattgcagt
     6541 aagctgagat cgcagcactg cactccagcc tgggcgacag agtaagactc tgtctcaaaa
     6601 ataaaataaa taaatcaatc agatattcca atcttttcct ttatttattt atttattttc
     6661 tattttggaa acacagtcct tccttattcc agaattacac atatattcta tttttcttta
     6721 tatgctccag ttttttttag accttcacct gaaatgtgtg tatcgagacc tagaaaaaca
     6781 tggagcaatc acaagtagca atacagcagc taacaatgct gcttgtgcct ggctagaagc
     6841 acaagaggag gaagaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac
     6901 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     6961 aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
     7021 cttccctgat tggcagaact acacaccagg gccaggggtc agatatccac tgacctttgg
     7081 atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga
     7141 gaacaccagc ttgttacacc ctgtgagcct gcatggaatg gatgaccctg agagagaagt
     7201 gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc
     7261 ggagtatcta gatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     7321 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     7381 tagggacttt ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     7441 ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg gcggtaggcg tgtactctag
     7501 atgctacata taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct
     7561 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     7621 cttgagtgct caaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     7681 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     7741 agcgaaagta aagccagagg agatctctcg acgcaggact cggcttgctg aagcgcgcac
     7801 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     7861 gaaggagaga gatgggtgcg agagcgtcgg tattaagcgg gggagaatta gataaatggg
     7921 aaaaaattcg gttaaggcca gggggaaaga aacaatataa actaaaacat atagtatggg
     7981 caagcaggga gctagaacga ttcgcagtta atcctggcct tttagagaca tcagaaggct
     8041 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     8101 cattatataa tacaatagca gtcctctatt gtgtgcatca aaggatagat gtaaaagaca
     8161 ccaaggaagc cttagataag atagaggaag agcaaaacaa aagtaagaaa aaggcacagc
     8221 aagcagcagc tgacacagga aacaacagcc aggtcagcca aaattaccct atagtgcaga
     8281 acctccaggg gcaaatggta catcaggcca tatcacctag aactttaaat atgactccgc
     8341 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     8401 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     8461 gaggcttttt tggaggccta ggcttttgca aatgcataaa agaaaagggg ggattggggg
     8521 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     8581 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
     8641 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     8701 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     8761 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     8821 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     8881 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     8941 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     9001 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     9061 aatctctgga acagatttgg aataacatga cctggatgga gtgggacaga gaaattaaca
     9121 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     9181 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     9241 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     9301 tagtttttgc tgtactttct gtagtgaata gagttaggca gggatattca ccattatcgt
     9361 ttcagaccca cctcccaatc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     9421 gtggagagag agacagagac agatccattc gattagtgaa cggatctagt taactagatc
     9481 tagagtc
//
