LOCUS       sequence-271274-       10132 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Second-generation lentiviral packaging plasmid lacking reverse 
            transcriptase activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10132)
  AUTHORS   Zhang XY, La Russa VF, Reiser J
  TITLE     Transduction of bone-marrow-derived mesenchymal stem cells by using 
            lentivirus vectors pseudotyped with modified RD114 envelope 
            glycoproteins.
  JOURNAL   J Virol. 2004 Feb . 78(3):1219-29.
  PUBMED    14722277
REFERENCE   2  (bases 1 to 10132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2004 Feb . 78(3):1219-29."
COMMENT     Sequence Label: pCD/NL-BH*deltavpu/RT-
FEATURES             Location/Qualifiers
     source          1..10132
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             684..2186
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /translation="MGARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGNNSQVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             1979..4990
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKKKKSVTVLEVGDAYFSVPLDEDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFKKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGLTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYPGIKVRQLCKLLRGTKALTEVIPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMRGAHT
                     NDVKQLTEAVQKITTESIVIWGKTPKFKLPIQKETWETWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIVGAETFYVDGAANRETKLGKAGYVTNKGRQKVVPLTNTTNQKTELQ
                     AIYLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVNQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    4675..4792
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     rep_origin      6190..6325
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    6493..6726
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6911..6955
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             7249..7275
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     primer_bind     complement(7654..7671)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7660..7884
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7972..7989)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8143..8731)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8223..8242)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8902..9693,9694..9762))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9525..9544
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9763..9867)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9942..9961)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatctctag aagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
      661 cgaggggcgg cgactggtga gagatgggtg cgagagcgtc ggtattaagc gggggagaat
      721 tagataaatg ggaaaaaatt cggttaaggc cagggggaaa gaaacaatat aaactaaaac
      781 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc cttttagaga
      841 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
      901 aagaacttag atcattatat aatacaatag cagtcctcta ttgtgtgcat caaaggatag
      961 atgtaaaaga caccaaggaa gccttagata agatagagga agagcaaaac aaaagtaaga
     1021 aaaaggcaca gcaagcagca gctgacacag gaaacaacag ccaggtcagc caaaattacc
     1081 ctatagtgca gaacctccag gggcaaatgg tacatcaggc catatcacct agaactttaa
     1141 atgcatgggt aaaagtagta gaagagaagg ctttcagccc agaagtaata cccatgtttt
     1201 cagcattatc agaaggagcc accccacaag atttaaatac catgctaaac acagtggggg
     1261 gacatcaagc agccatgcaa atgttaaaag agaccatcaa tgaggaagct gcagaatggg
     1321 atagattgca tccagtgcat gcagggccta ttgcaccagg ccagatgaga gaaccaaggg
     1381 gaagtgacat agcaggaact actagtaccc ttcaggaaca aataggatgg atgacacata
     1441 atccacctat cccagtagga gaaatctata aaagatggat aatcctggga ttaaataaaa
     1501 tagtaagaat gtatagccct accagcattc tggacataag acaaggacca aaggaaccct
     1561 ttagagacta tgtagaccga ttctataaaa ctctaagagc cgagcaagct tcacaagagg
     1621 taaaaaattg gatgacagaa accttgttgg tccaaaatgc gaacccagat tgtaagacta
     1681 ttttaaaagc attgggacca ggagcgacac tagaagaaat gatgacagca tgtcagggag
     1741 tggggggacc cggccataaa gcaagagttt tggctgaagc aatgagccaa gtaacaaatc
     1801 cagctaccat aatgatacag aaaggcaatt ttaggaacca aagaaagact gttaagtgtt
     1861 tcaattgtgg caaagaaggg cacatagcca aaaattgcag ggcccctagg aaaaagggct
     1921 gttggaaatg tggaaaggaa ggacaccaaa tgaaagattg tactgagaga caggctaatt
     1981 ttttagggaa gatctggcct tcccacaagg gaaggccagg gaattttctt cagagcagac
     2041 cagagccaac agccccacca gaagagagct tcaggtttgg ggaagagaca acaactccct
     2101 ctcagaagca ggagccgata gacaaggaac tgtatccttt agcttccctc agatcactct
     2161 ttggcagcga cccctcgtca caataaagat aggggggcaa ttaaaggaag ctctattaga
     2221 tacaggagca gatgatacag tattagaaga aatgaatttg ccaggaagat ggaaaccaaa
     2281 aatgataggg ggaattggag gttttatcaa agtaagacag tatgatcaga tactcataga
     2341 aatctgcgga cataaagcta taggtacagt attagtagga cctacacctg tcaacataat
     2401 tggaagaaat ctgttgactc agattggctg cactttaaat tttcccatta gtcctattga
     2461 gactgtacca gtaaaattaa agcccgggat ggatggccca aaagttaaac aatggccatt
     2521 gacagaagaa aaaataaaag cattagtaga aatttgtaca gaaatggaaa aggaagggaa
     2581 aatttcaaaa attgggcctg agaatccata caatactcca gtatttgcca taaagaaaaa
     2641 agacagtact aaatggagaa aattagtaga tttcagagaa cttaataaga gaactcaaga
     2701 cttctgggaa gttcaattag gaataccaca tcccgcaggg ttaaaaaaga aaaaatcagt
     2761 aacagtactg gaagtgggtg atgcatattt ttcagttccc ttagatgaag acttcaggaa
     2821 gtatactgca tttaccatac ctagtataaa caatgagaca ccagggatta gatatcagta
     2881 caatgtgctt ccacagggat ggaaaggatc accagcaata ttccaaagta gcatgacaaa
     2941 aatcttagag ccttttaaaa aacaaaatcc agacatagtt atctatcaat acatggatga
     3001 tttgtatgta ggatctgact tagaaatagg gcagcataga acaaaaatag aggagctgag
     3061 acaacatctg ttgaggtggg gacttaccac accagacaaa aaacatcaga aagaacctcc
     3121 attcctttgg atgggttatg aactccatcc tgataaatgg acagtacagc ctatagtgct
     3181 gccagaaaaa gacagctgga ctgtcaatga catacagaag ttagtgggga aattgaattg
     3241 ggcaagtcag atttacccag ggattaaagt aaggcaatta tgtaaactcc ttagaggaac
     3301 caaagcacta acagaagtaa taccactaac agaagaagca gagctagaac tggcagaaaa
     3361 cagagagatt ctaaaagaac cagtacatgg agtgtattat gacccatcaa aagacttaat
     3421 agcagaaata cagaagcagg ggcaaggcca atggacatat caaatttatc aagagccatt
     3481 taaaaatctg aaaacaggaa aatatgcaag aatgaggggt gcccacacta atgatgtaaa
     3541 acaattaaca gaggcagtgc aaaaaataac cacagaaagc atagtaatat ggggaaagac
     3601 tcctaaattt aaactaccca tacaaaagga aacatgggaa acatggtgga cagagtattg
     3661 gcaagccacc tggattcctg agtgggagtt tgttaatacc cctcctttag tgaaattatg
     3721 gtaccagtta gagaaagaac ccatagtagg agcagaaacc ttctatgtag atggggcagc
     3781 taacagggag actaaattag gaaaagcagg atatgttact aacaaaggaa gacaaaaggt
     3841 tgtcccccta actaacacaa caaatcagaa aactgagtta caagcaattt atctagcttt
     3901 gcaggattca ggattagaag taaacatagt aacagactca caatatgcat taggaatcat
     3961 tcaagcacaa ccagataaaa gtgaatcaga gttagtcaat caaataatag agcagttaat
     4021 taaaaaggaa aaggtctatc tggcatgggt gccggcacac aaaggaattg gaggaaatga
     4081 acaagtagat aaattggtca gtgctggaat caggaaagta ctatttttag atggaataga
     4141 taaggcccaa gaagaacatg agaaatatca cagtaattgg agagcaatgg ctagtgattt
     4201 taacctacca cctgtagtag caaaagaaat agtagccagc tgtgataaat gtcagctaaa
     4261 aggggaagcc atgcatggac aagtagactg tagcccagga atatggcagc tagattgtac
     4321 acatttagaa ggaaaagtta tcttggtagc agttcatgta gccagtggat atatagaagc
     4381 agaagtaatt ccagcagaga cagggcaaga aacagcatac ttcctcttaa aattagcagg
     4441 aagatggcca gtaaaaacag tacatacaga caatggcagc aatttcacca gtactacagt
     4501 taaggccgcc tgttggtggg cggggatcaa gcaggaattt ggcattccct acaatcccca
     4561 aagtcaagga gtaatagaat ctatgaataa agaattaaag aaaattatag gacaggtaag
     4621 agatcaggct gaacatctta agacagcagt acaaatggca gtattcatcc acaattttaa
     4681 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     4741 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
     4801 ttacagggac agcagagatc cagtttggaa aggaccagca aagctcctct ggaaaggtga
     4861 aggggcagta gtaatacaag ataatagtga cataaaagta gtgccaagaa gaaaagcaaa
     4921 gatcatcagg gattatggaa aacagatggc aggtgatgat tgtgtggcaa gtagacagga
     4981 tgaggattaa cacatggaaa agattagtaa aacaccatat gtatatttca aggaaagcta
     5041 aggactggtt ttatagacat cactatgaaa gtactaatcc aaaaataagt tcagaagtac
     5101 acatcccact aggggatgct aaattagtaa taacaacata ttggggtctg catacaggag
     5161 aaagagactg gcatttgggt cagggagtct ccatagaatg gaggaaaaag agatatagca
     5221 cacaagtaga ccctgaccta gcagaccaac taattcatct gcactatttt gattgttttt
     5281 cagaatctgc tataagaaat accatattag gacgtatagt tagtcctagg tgtgaatatc
     5341 aagcaggaca taacaaggta ggatctctac agtacttggc actagcagca ttaataaaac
     5401 caaaacagat aaagccacct ttgcctagtg ttaggaaact gacagaggac agatggaaca
     5461 agccccagaa gaccaagggc cacagaggga gccatacaat gaatggacac tagagctttt
     5521 agaggaactt aagagtgaag ctgttagaca ttttcctagg atatggctcc ataacttagg
     5581 acaacatatc tatgaaactt acggggatac ttgggcagga gtggaagcca taataagaat
     5641 tctgcaacaa ctgctgttta tccatttcag aattgggtgt cgacatagca gaataggcgt
     5701 tactcgacag aggagagcaa gaaatggagc cagtagatcc tagactagag ccctggaagc
     5761 atccaggaag tcagcctaaa actgcttgta ccaattgcta ttgtaaaaag tgttgctttc
     5821 attgccaagt ttgtttcatg acaaaagcct taggcatctc ctatggcagg aagaagcgga
     5881 gacagcgacg aagagctcat cagaacagtc agactcatca agcttctcta tcaaagcagt
     5941 aagtagtaca tgtaatttaa ttgatagact aatagaaaga gcagaagaca gtggcaatga
     6001 gagtgaagga gaagtatcag cacttgtgga gatgggggtg gaaatggggc accatgctcc
     6061 ttgggatatt gatgatctgt agtgctacag aaaaattgtg ggtcacagtc tattatgggg
     6121 tacctgtgtg gaaggaagca accaccactc tattttgtgc atcagatgct aaagcatatg
     6181 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     6241 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     6301 tagtgaggag gcttttttgg aggcctaggc ttttgcaaat gcatagatct tcagacctgg
     6361 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     6421 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     6481 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     6541 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     6601 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     6661 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     6721 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     6781 tgctagttgg agtaataaat ctctggaaca gatttggaat aacatgacct ggatggagtg
     6841 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     6901 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     6961 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     7021 cttggtaggt ttaagaatag tttttgctgt actttctgta gtgaatagag ttaggcaggg
     7081 atattcacca ttatcgtttc agacccacct cccaatcccg aggggacccg acaggcccga
     7141 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     7201 atccttggca cttatctggg acgatctgcg gagcctgtgc ctcttcagct accaccgctt
     7261 gagagactta ctcttgattg taacgaggat tgtggaactt ctgggacgca gggggtggga
     7321 agccctcaaa tattggtgga atctcctaca gtattggagt caggaactaa agaatagtgc
     7381 tgttagcttg ctcaatgcca cagccatagc agtagctgag gggacagata gggttataga
     7441 agtagtacaa ggagcttgta gagctattcg ccacatacct agaagaataa gacagggctt
     7501 ggaaaggatt ttgctataag atgggtggca agtggtcaaa aagtagtgtg attggatggc
     7561 ctactgtaag ggaaagaatg agacgagctg agccagcagc agatagggtg ggagcagcat
     7621 ctcgagtcta gagcgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     7681 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     7741 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     7801 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     7861 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgc attaatgaat
     7921 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     7981 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     8041 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     8101 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     8161 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     8221 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     8281 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     8341 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     8401 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     8461 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     8521 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     8581 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     8641 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     8701 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     8761 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     8821 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     8881 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     8941 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     9001 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     9061 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     9121 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     9181 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     9241 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     9301 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     9361 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     9421 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     9481 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     9541 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     9601 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     9661 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     9721 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     9781 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     9841 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
     9901 cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc
     9961 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
    10021 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
    10081 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg tt
//
