LOCUS       sequence-27128-e         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Tritschler F, Eulalio A, Truffault V, Hartmann MD, Helms S, Schmidt 
            S, Coles M, Izaurralde E, Weichenrieder O
  TITLE     A divergent Sm fold in EDC3 proteins mediates DCP1 binding and 
            P-body targeting.
  JOURNAL   Mol Cell Biol. 2007 Dec . 27(24):8600-11.
  PUBMED    17923697
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2007 Dec . 27(24):8600-11."
COMMENT     Sequence Label: pAc5.1B-EGFP
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             79..120
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             130..147
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(170..187)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    220..354
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(244..263)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     298..317
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tacaagccaa gcttgaattc tgcagatatc cagcacagnt ggcggccgct tcgagntcta
       61 gagggcccgc ggttcgaagg taagcctatc cctaaccctc tcctcggtct cgattctacg
      121 cgtaccggtc atcatcacca tcaccattga gtttaaaccc gctgatcagc ctcgactgtg
      181 ccttctaagg cctgagctcg ctgatcagcc tcgatcgagg atccagacat gataagatac
      241 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
      301 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
      361 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc
      421 aagtaaaacc tctacaaatg tggtatggct gattatgatc agtcgaccga tgcccttgag
      481 agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact
      541 tatgactgtc ttctttatca tgcaactcgt angacaggtg ccggcagcgc tctgggtcat
      601 tttcggcgag gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt
      661 cggaatcttg cacgccctcg ctcaagcctt cgtcactggt cccgccacca aacgtttcgg
      721 cgagaagcag gcaatatcgc cggcatg
//
