LOCUS       sequence-271288-        8522 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CROP-seq vector optimized for sgRNA expression and CRISPRi activity.
            This vector expresses a eGFP-NT2 sgRNA with modified U6 promotor and
            sgRNA constant region. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8522)
  AUTHORS   Replogle JM, Norman TM, Xu A, Hussmann JA, Chen J, Cogan JZ, Meer 
            EJ, Terry JM, Riordan DP, Srinivas N, Fiddes IT, Arthur JG, Alvarado
            LJ, Pfeiffer KA, Mikkelsen TS, Weissman JS, Adamson B
  TITLE     Combinatorial single-cell CRISPR screens by direct guide RNA capture
            and targeted sequencing.
  JOURNAL   Nat Biotechnol. 2020 Aug;38(8):954-961. doi: 
            10.1038/s41587-020-0470-y. Epub 2020 Mar 30.
  PUBMED    32231336
REFERENCE   2  (bases 1 to 8522)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0470-y"; journalName: "Nat Biotechnol"; date: 
            "2020-08"; volume: "38"; issue: "8"; pages: "954-961"
COMMENT     Sequence Label: pBA950
FEATURES             Location/Qualifiers
     source          1..8522
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    88..109
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(124..129,130..147,148..154)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    162..178
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     167..189
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     186..202
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     186..202
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     221..241
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        223..241
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        269..495
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             496..676
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    723..848
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1341..1574
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1759..1803
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1984..2101
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2240..3418
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2471..3409
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3366..3386
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3431..4132
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    4148..4736
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4201..4221)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4619..4630)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(5092..5125)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     5129..5149
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     LTR             5377..5557
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    5635..5756
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5672..5691)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5726..5745
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5775..5910
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5930..5949)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5931..5949)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5959..5976)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5959..5975)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5968..5990)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6117..6572
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6204..6223)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6414..6435
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6598..6702
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6703..6771,6772..7563)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6921..6940)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7734..8322
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8223..8242
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8476..8493
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
       61 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
      121 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
      181 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
      241 ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc
      301 aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc
      361 cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg
      421 acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta
      481 gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc
      541 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
      601 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
      661 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
      721 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
      781 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
      841 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
      901 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
      961 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     1021 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     1081 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     1141 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc
     1201 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     1261 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     1321 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     1381 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     1441 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     1501 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     1561 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     1621 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     1681 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     1741 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     1801 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     1861 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     1921 aggcagggat attcaccatt atcgtttcag aatcttgaga caaatggcag tattcatcca
     1981 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     2041 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     2101 tcgggtttat tacagggaca gcagagatcc actttggcgc cggctcgagg gggcccgggt
     2161 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
     2221 cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     2281 agtccccgag aagttggggg gaggggtcgg caattgatcc ggtgcctaga gaaggtggcg
     2341 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     2401 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     2461 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg
     2521 cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct
     2581 tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg
     2641 tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct
     2701 tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc
     2761 tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg
     2821 tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc
     2881 ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg
     2941 ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag
     3001 gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc
     3061 agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca
     3121 aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc
     3181 gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg
     3241 ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc
     3301 agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt
     3361 cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgacg
     3421 tacggccacc atgagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg
     3481 caccgtggac aaccatcact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg
     3541 cacccagacc atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat
     3601 cctggctact agcttcctct acggcagcaa gaccttcatc aaccacaccc agggcatccc
     3661 cgacttcttc aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga
     3721 agacgggggc gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta
     3781 caacgtcaag atcagagggg tgaacttcac atccaacggc cctgtgatgc agaagaaaac
     3841 actcggctgg gaggccttca ccgagaccct gtaccccgct gacggcggcc tggaaggcag
     3901 aaacgacatg gccctgaagc tcgtgggcgg gagccatctg atcgcaaaca tcaagaccac
     3961 atatagatcc aagaaacccg ctaagaacct caagatgcct ggcgtctact atgtggacta
     4021 cagactggaa agaatcaagg aggccaacaa cgagacctac gtcgagcagc acgaggtggc
     4081 agtggccaga tactgcgacc tccctagcaa actggggcac aagcttaatt aaacgcgtta
     4141 agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     4201 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     4261 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     4321 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     4381 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     4441 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     4501 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     4561 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     4621 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     4681 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg
     4741 actttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     4801 gggggactgg aagggctaat tcactcccaa cgaagacaag atttcaccat tatcgtttca
     4861 gacccacctc ccaaccccga ggggacccag atctagagat ccgacgcgcc atctctaggc
     4921 ccgcgccggc cccctcgcac ggacttgtgg gagaagctcg gctactcccc tgccccggtt
     4981 aatttgcata taatatttcc tagtaactat agaggcttaa tgtgcgataa aagacagata
     5041 atctgttctt tttaatacta gctacatttt acatgatagg cttggatttc tataacttcg
     5101 tatagcatac attatacgaa gttataaaca gcacaaaagg aaactcaccc taactgtaaa
     5161 gtaattgtgt gttttgagac tataagtatc ccttggagaa ccaccttgtt ggaccaggat
     5221 gggcaccacc cgtttaagag ctaagctgga aacagcatag caagtttaaa taaggctagt
     5281 ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt ttttctcgag aagcttggcg
     5341 taactagatc ttgagacact gctttttgct tgtactgggt ctctctggtt agaccagatc
     5401 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     5461 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     5521 ctcagaccct tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat
     5581 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     5641 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     5701 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     5761 gtctggctct agctatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     5821 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     5881 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
     5941 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     6001 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     6061 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     6121 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     6181 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     6241 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     6301 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     6361 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     6421 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     6481 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     6541 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     6601 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     6661 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     6721 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     6781 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     6841 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     6901 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     6961 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     7021 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     7081 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     7141 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     7201 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     7261 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     7321 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     7381 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     7441 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     7501 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     7561 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     7621 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     7681 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     7741 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     7801 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     7861 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     7921 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     7981 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     8041 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     8101 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     8161 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     8221 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     8281 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     8341 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     8401 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     8461 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     8521 cc
//
