LOCUS       sequence-27130-e         710 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Rehwinkel J, Behm-Ansmant I, Gatfield D, Izaurralde E
  TITLE     A crucial role for GW182 and the DCP1:DCP2 decapping complex in 
            miRNA-mediated gene silencing.
  JOURNAL   RNA. 2005 Nov . 11(11):1640-7.
  PUBMED    16177138
REFERENCE   2  (bases 1 to 710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2005 
            Nov . 11(11):1640-7."
COMMENT     Sequence Label: pAc5.1C-RLuc-V5His6
FEATURES             Location/Qualifiers
     source          1..710
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..49
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(72..89)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(99..140)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 tcgatcgagg ctgatcagcg agctcaggcc ttagaaggca cagtcgaggc tgatcagcgg
       61 gtttaaactc aatggtgatg gtgatgatga ccggtacgcg tagaatcgag accgaggaga
      121 gggttaggga taggcttacc ttcgaatggg tgacctcgag cttgttcagt tttgagaact
      181 cgctcaacga acgatttgat atattttccc atttcatcag gtgcatcttc ttgcgaaaaa
      241 tgaagacctt ttactttgac aaattcagta ttaggaaact tcttggcacc ttcaacaata
      301 gcattggaaa agaatcctgg gtccgattca ataaacattt ttggtaaatc atcacttgca
      361 cgtagataag cattataatt cctaacaatt tgtacaacgt caggtttacc accttttact
      421 aacgggattt cacgaggcca tgataatgtt ggacgacgaa cttcaccttt ctctttgaat
      481 ggttcaagat atgctgcaaa ttcttctggt tctaactttc tcatgatttt tgatggcaac
      541 atggtttcca cgaagaagtt attctccaaa accatttttt ctccttcttc agatttgatc
      601 aacgcaatat cttcttcaat atcaggccat tcatcccatg attcaatcac atctactaca
      661 ctttcagcgt gaactattgc tttgatctta tcttgatgct catagctata
//
