LOCUS       sequence-27133-e         742 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Rehwinkel J, Behm-Ansmant I, Gatfield D, Izaurralde E
  TITLE     A crucial role for GW182 and the DCP1:DCP2 decapping complex in 
            miRNA-mediated gene silencing.
  JOURNAL   RNA. 2005 Nov . 11(11):1640-7.
  PUBMED    16177138
REFERENCE   2  (bases 1 to 742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2005 
            Nov . 11(11):1640-7."
COMMENT     Sequence Label: pAc5.1C-FLuc-V5His6
FEATURES             Location/Qualifiers
     source          1..742
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             110..151
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             161..178
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(201..218)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    251..385
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(275..294)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     329..348
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gagtccgaaa ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga tcctcataaa
       61 ggccaagaag ggcggaaagt ccaaattggc tcgaggtcac ccattcgaag gtaagcctat
      121 ccctaaccct ctcctcggtc tcgattctac gcgtaccggt catcatcacc atcaccattg
      181 agtttaaacc cgctgatcag cctcgactgt gccttctaag gcctgagctc gctgatcagc
      241 ctcgatcgag gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
      301 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
      361 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
      421 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc
      481 tgattatgat cagtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt
      541 gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg
      601 taggacaggt gccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg
      661 cgacgatgat cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct
      721 tcgtcactgg tcccgccacc aa
//
